Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States

Abstract We measured concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants, influenza A and B viruses, respiratory syncytial virus, human metapneumovirus, enterovirus D68, human parainfluenza types 1, 2, 3, 4a, and 4b in aggregate, norovirus genotype II, rot...

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Main Authors: Alexandria B. Boehm, Marlene K. Wolfe, Amanda L. Bidwell, Alessandro Zulli, Vikram Chan-Herur, Bradley J. White, Bridgette Shelden, Dorothea Duong
Format: Article
Language:English
Published: Nature Portfolio 2024-10-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-024-03969-8
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author Alexandria B. Boehm
Marlene K. Wolfe
Amanda L. Bidwell
Alessandro Zulli
Vikram Chan-Herur
Bradley J. White
Bridgette Shelden
Dorothea Duong
author_facet Alexandria B. Boehm
Marlene K. Wolfe
Amanda L. Bidwell
Alessandro Zulli
Vikram Chan-Herur
Bradley J. White
Bridgette Shelden
Dorothea Duong
author_sort Alexandria B. Boehm
collection DOAJ
description Abstract We measured concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants, influenza A and B viruses, respiratory syncytial virus, human metapneumovirus, enterovirus D68, human parainfluenza types 1, 2, 3, 4a, and 4b in aggregate, norovirus genotype II, rotavirus, Candida auris, hepatitis A virus, human adenovirus, mpox virus, H5 influenza A virus, and pepper mild mottle virus nucleic acids in wastewater solids prospectively at 191 wastewater treatment plants in 40 states across the United States plus Washington DC. Measurements were made two to seven times per week from 1 January 2022 to 30 June 2024, depending on wastewater treatment plant staff availability. Measurements were made using droplet digital (reverse-transcription–) polymerase chain reaction (ddRT-PCR) following best practices for making environmental molecular biology measurements. These data can be used to better understand disease occurrence in communities contributing to the wastewater.
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spelling doaj-art-461b0931a3bd46e4bcb948d8777285a22025-08-20T02:05:46ZengNature PortfolioScientific Data2052-44632024-10-0111111410.1038/s41597-024-03969-8Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United StatesAlexandria B. Boehm0Marlene K. Wolfe1Amanda L. Bidwell2Alessandro Zulli3Vikram Chan-Herur4Bradley J. White5Bridgette Shelden6Dorothea Duong7Department of Civil & Environmental Engineering, School of Engineering and Doerr School of Sustainability, Stanford UniversityGangarosa Department of Environmental Health, Rollins School of Public Health, Emory UniversityDepartment of Civil & Environmental Engineering, School of Engineering and Doerr School of Sustainability, Stanford UniversityDepartment of Civil & Environmental Engineering, School of Engineering and Doerr School of Sustainability, Stanford UniversityVerily Life SciencesVerily Life SciencesVerily Life SciencesVerily Life SciencesAbstract We measured concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants, influenza A and B viruses, respiratory syncytial virus, human metapneumovirus, enterovirus D68, human parainfluenza types 1, 2, 3, 4a, and 4b in aggregate, norovirus genotype II, rotavirus, Candida auris, hepatitis A virus, human adenovirus, mpox virus, H5 influenza A virus, and pepper mild mottle virus nucleic acids in wastewater solids prospectively at 191 wastewater treatment plants in 40 states across the United States plus Washington DC. Measurements were made two to seven times per week from 1 January 2022 to 30 June 2024, depending on wastewater treatment plant staff availability. Measurements were made using droplet digital (reverse-transcription–) polymerase chain reaction (ddRT-PCR) following best practices for making environmental molecular biology measurements. These data can be used to better understand disease occurrence in communities contributing to the wastewater.https://doi.org/10.1038/s41597-024-03969-8
spellingShingle Alexandria B. Boehm
Marlene K. Wolfe
Amanda L. Bidwell
Alessandro Zulli
Vikram Chan-Herur
Bradley J. White
Bridgette Shelden
Dorothea Duong
Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States
Scientific Data
title Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States
title_full Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States
title_fullStr Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States
title_full_unstemmed Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States
title_short Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States
title_sort human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the united states
url https://doi.org/10.1038/s41597-024-03969-8
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