miRNA-451a and miRNA-125a Expression Levels in Ankylosing Spondylitis: Impact on Disease Diagnosis, Prognosis, and Outcomes
Background. Early diagnosis of ankylosing spondylitis (AS) is yet not elucidated, with a potential diagnostic glance of microRNAs (miRNAs). Aim. Study the expression profile of miRNA-451a and miRNA-125a in AS patients and their impact on disease activity and prognosis. Methods. A cross-sectional stu...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/2180913 |
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| author | Dina Salem Fotoh Rasha Ibrahim Noreldin Mohamed Soliman Rizk Maha Mohamed Elsabaawy Heba Ahmed Esaily |
| author_facet | Dina Salem Fotoh Rasha Ibrahim Noreldin Mohamed Soliman Rizk Maha Mohamed Elsabaawy Heba Ahmed Esaily |
| author_sort | Dina Salem Fotoh |
| collection | DOAJ |
| description | Background. Early diagnosis of ankylosing spondylitis (AS) is yet not elucidated, with a potential diagnostic glance of microRNAs (miRNAs). Aim. Study the expression profile of miRNA-451a and miRNA-125a in AS patients and their impact on disease activity and prognosis. Methods. A cross-sectional study included 55 AS patients diagnosed according to modified New York criteria in 1984 with 55 matched healthy controls. History, clinical examination, and disease activity assessment with Bath ankylosing spondylitis disease activity index (BASDAI) were done. Full laboratory and radiological assessment along with expression profile of m iRNA-451a and miRNA-125a were tabulated and analyzed. Results. Higher expression levels of miRNA-125a and lower of miRNA-451a in AS patients compared to controls. Furthermore, miRNA-125a expression was high in active AS patients compared to inactive patients and controls (7.0±3.4 vs. 4.1±2.1 vs. 2.6±0.6, p<0.001, respectively). miRNA-451a was significantly lower in active AS patients compared to inactive patients and controls (2.2±1.1 vs. 4.1±2.3 vs. 7.1±4.5, respectively). Both miRNAs (miRNA-125a and miRNA-451a) had evident accuracy for AS diagnosis with areas under the curve (AUC) of 0.788 and 0.802, respectively. miRNA-125a had potential impact on AS activity with AUC of 0.777. Plasma levels of both miRNAs were able to distinguish AS patients with structural damage with AUCs 0.775 and 0.692, respectively. Conclusions. Both miRNA-451a and miRNA-125a were found to be of great value as sensitive noninvasive diagnostic, prognostic, and disease burden biomarker of AS patients in Egypt with suggested further studies for future therapeutic implications. |
| format | Article |
| id | doaj-art-4606d30d971947d590a97209f39aeb84 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-4606d30d971947d590a97209f39aeb842025-02-03T01:28:07ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/21809132180913miRNA-451a and miRNA-125a Expression Levels in Ankylosing Spondylitis: Impact on Disease Diagnosis, Prognosis, and OutcomesDina Salem Fotoh0Rasha Ibrahim Noreldin1Mohamed Soliman Rizk2Maha Mohamed Elsabaawy3Heba Ahmed Esaily4Physical Medicine, Rheumatology and Rehabilitation Department, Faculty of Medicine, Menoufia University, EgyptClinical Pathology Department, Faculty of Medicine, Menoufia University, EgyptMedical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, EgyptHepatology and Gastroenterology Department, National Liver Institute, Menoufia University, EgyptPhysical Medicine, Rheumatology and Rehabilitation Department, Faculty of Medicine, Menoufia University, EgyptBackground. Early diagnosis of ankylosing spondylitis (AS) is yet not elucidated, with a potential diagnostic glance of microRNAs (miRNAs). Aim. Study the expression profile of miRNA-451a and miRNA-125a in AS patients and their impact on disease activity and prognosis. Methods. A cross-sectional study included 55 AS patients diagnosed according to modified New York criteria in 1984 with 55 matched healthy controls. History, clinical examination, and disease activity assessment with Bath ankylosing spondylitis disease activity index (BASDAI) were done. Full laboratory and radiological assessment along with expression profile of m iRNA-451a and miRNA-125a were tabulated and analyzed. Results. Higher expression levels of miRNA-125a and lower of miRNA-451a in AS patients compared to controls. Furthermore, miRNA-125a expression was high in active AS patients compared to inactive patients and controls (7.0±3.4 vs. 4.1±2.1 vs. 2.6±0.6, p<0.001, respectively). miRNA-451a was significantly lower in active AS patients compared to inactive patients and controls (2.2±1.1 vs. 4.1±2.3 vs. 7.1±4.5, respectively). Both miRNAs (miRNA-125a and miRNA-451a) had evident accuracy for AS diagnosis with areas under the curve (AUC) of 0.788 and 0.802, respectively. miRNA-125a had potential impact on AS activity with AUC of 0.777. Plasma levels of both miRNAs were able to distinguish AS patients with structural damage with AUCs 0.775 and 0.692, respectively. Conclusions. Both miRNA-451a and miRNA-125a were found to be of great value as sensitive noninvasive diagnostic, prognostic, and disease burden biomarker of AS patients in Egypt with suggested further studies for future therapeutic implications.http://dx.doi.org/10.1155/2020/2180913 |
| spellingShingle | Dina Salem Fotoh Rasha Ibrahim Noreldin Mohamed Soliman Rizk Maha Mohamed Elsabaawy Heba Ahmed Esaily miRNA-451a and miRNA-125a Expression Levels in Ankylosing Spondylitis: Impact on Disease Diagnosis, Prognosis, and Outcomes Journal of Immunology Research |
| title | miRNA-451a and miRNA-125a Expression Levels in Ankylosing Spondylitis: Impact on Disease Diagnosis, Prognosis, and Outcomes |
| title_full | miRNA-451a and miRNA-125a Expression Levels in Ankylosing Spondylitis: Impact on Disease Diagnosis, Prognosis, and Outcomes |
| title_fullStr | miRNA-451a and miRNA-125a Expression Levels in Ankylosing Spondylitis: Impact on Disease Diagnosis, Prognosis, and Outcomes |
| title_full_unstemmed | miRNA-451a and miRNA-125a Expression Levels in Ankylosing Spondylitis: Impact on Disease Diagnosis, Prognosis, and Outcomes |
| title_short | miRNA-451a and miRNA-125a Expression Levels in Ankylosing Spondylitis: Impact on Disease Diagnosis, Prognosis, and Outcomes |
| title_sort | mirna 451a and mirna 125a expression levels in ankylosing spondylitis impact on disease diagnosis prognosis and outcomes |
| url | http://dx.doi.org/10.1155/2020/2180913 |
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