The changes of immune function after neuroblastoma chemotherapy and the prediction of potential immune targets
Abstract Background High-dose chemotherapy for neuroblastoma is often intolerable for children, and its effectiveness is difficult to determine. Immunotherapy has become a popular research focus as a potential treatment. Therefore, identifying effective immune targets and drug synergistic chemothera...
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BMC
2025-08-01
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| Series: | BMC Medical Genomics |
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| Online Access: | https://doi.org/10.1186/s12920-025-02185-6 |
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| author | Yunfei Ma Yan Su Jing Huang Wen Zhao Cheng Huang Jing Qin Shengjie You Yan Hu Xin Ni |
| author_facet | Yunfei Ma Yan Su Jing Huang Wen Zhao Cheng Huang Jing Qin Shengjie You Yan Hu Xin Ni |
| author_sort | Yunfei Ma |
| collection | DOAJ |
| description | Abstract Background High-dose chemotherapy for neuroblastoma is often intolerable for children, and its effectiveness is difficult to determine. Immunotherapy has become a popular research focus as a potential treatment. Therefore, identifying effective immune targets and drug synergistic chemotherapy against neuroblastoma is crucial. Methods We conducted proteomics exploratory analysis of urine from 12 neuroblastoma before and after chemotherapy. The immune-related differential proteins before and after chemotherapy were obtained through differential expression analysis and dynamic expression model analysis. The ESTIMATE and ssGSEA analyses used indirectly infer the characteristics of the immune microenvironment. Gene ontology and the Kyoto Encyclopedia of Genes and Genomes enrichment analysis were conducted to reveal the biological functions of the candidate genes. Results Immune analysis indicated that the immunosuppressive state in neuroblastoma patients caused by chemotherapy was closely related to CD8 T cells. Chemotherapy-associated differentially expressed proteins were significantly enriched in the mTOR signaling pathway. Neuroblastoma chemotherapy can significantly inhibit the expression of SEMA7A protein, and it is closely related to CD8T cell infiltration. Tetrachlorodibenzodioxin acting on SEMA7A was predicted using CTD databases. Conclusions This study combines proteomics and bioinformatics to predict and explore potential immune targets for synergistic chemotherapy against neuroblastoma, providing a new direction for clinical treatment that warrant further validation. |
| format | Article |
| id | doaj-art-460253a2b31d4794be1e3de770dae2ce |
| institution | Kabale University |
| issn | 1755-8794 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medical Genomics |
| spelling | doaj-art-460253a2b31d4794be1e3de770dae2ce2025-08-24T11:56:20ZengBMCBMC Medical Genomics1755-87942025-08-0118111310.1186/s12920-025-02185-6The changes of immune function after neuroblastoma chemotherapy and the prediction of potential immune targetsYunfei Ma0Yan Su1Jing Huang2Wen Zhao3Cheng Huang4Jing Qin5Shengjie You6Yan Hu7Xin Ni8Department of Traditional Chinese Medicine, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthDepartment of Medical Oncology, Pediatric Oncology Center, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthPediatric Deparment of Xiyuan Hospital, China Academy of Chinese Medical SciencesDepartment of Medical Oncology, Pediatric Oncology Center, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthDepartment of Medical Oncology, Pediatric Oncology Center, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthDepartment of Medical Oncology, Pediatric Oncology Center, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthDepartment of Traditional Chinese Medicine, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthDepartment of Traditional Chinese Medicine, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthDepartment of National Center for Pediatric Cancer Surveillance, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s HealthAbstract Background High-dose chemotherapy for neuroblastoma is often intolerable for children, and its effectiveness is difficult to determine. Immunotherapy has become a popular research focus as a potential treatment. Therefore, identifying effective immune targets and drug synergistic chemotherapy against neuroblastoma is crucial. Methods We conducted proteomics exploratory analysis of urine from 12 neuroblastoma before and after chemotherapy. The immune-related differential proteins before and after chemotherapy were obtained through differential expression analysis and dynamic expression model analysis. The ESTIMATE and ssGSEA analyses used indirectly infer the characteristics of the immune microenvironment. Gene ontology and the Kyoto Encyclopedia of Genes and Genomes enrichment analysis were conducted to reveal the biological functions of the candidate genes. Results Immune analysis indicated that the immunosuppressive state in neuroblastoma patients caused by chemotherapy was closely related to CD8 T cells. Chemotherapy-associated differentially expressed proteins were significantly enriched in the mTOR signaling pathway. Neuroblastoma chemotherapy can significantly inhibit the expression of SEMA7A protein, and it is closely related to CD8T cell infiltration. Tetrachlorodibenzodioxin acting on SEMA7A was predicted using CTD databases. Conclusions This study combines proteomics and bioinformatics to predict and explore potential immune targets for synergistic chemotherapy against neuroblastoma, providing a new direction for clinical treatment that warrant further validation.https://doi.org/10.1186/s12920-025-02185-6NeuroblastomaChemotherapyProteomicsImmune functionBioinformatics |
| spellingShingle | Yunfei Ma Yan Su Jing Huang Wen Zhao Cheng Huang Jing Qin Shengjie You Yan Hu Xin Ni The changes of immune function after neuroblastoma chemotherapy and the prediction of potential immune targets BMC Medical Genomics Neuroblastoma Chemotherapy Proteomics Immune function Bioinformatics |
| title | The changes of immune function after neuroblastoma chemotherapy and the prediction of potential immune targets |
| title_full | The changes of immune function after neuroblastoma chemotherapy and the prediction of potential immune targets |
| title_fullStr | The changes of immune function after neuroblastoma chemotherapy and the prediction of potential immune targets |
| title_full_unstemmed | The changes of immune function after neuroblastoma chemotherapy and the prediction of potential immune targets |
| title_short | The changes of immune function after neuroblastoma chemotherapy and the prediction of potential immune targets |
| title_sort | changes of immune function after neuroblastoma chemotherapy and the prediction of potential immune targets |
| topic | Neuroblastoma Chemotherapy Proteomics Immune function Bioinformatics |
| url | https://doi.org/10.1186/s12920-025-02185-6 |
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