Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries
Specific binders comprised of nucleic acids, that is, RNA/DNA aptamers, are attractive functional biopolymers owing to their potential broad application in medicine, food hygiene, environmental analysis, and biological research. Despite the large number of reports on selection of natural DNA/RNA apt...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.1155/2012/156482 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849406892162416640 |
|---|---|
| author | Yuuya Kasahara Masayasu Kuwahara |
| author_facet | Yuuya Kasahara Masayasu Kuwahara |
| author_sort | Yuuya Kasahara |
| collection | DOAJ |
| description | Specific binders comprised of nucleic acids, that is, RNA/DNA aptamers, are attractive functional biopolymers owing to their potential broad application in medicine, food hygiene, environmental analysis, and biological research. Despite the large number of reports on selection of natural DNA/RNA aptamers, there are not many examples of direct screening of chemically modified nucleic acid aptamers. This is because of (i) the inferior efficiency and accuracy of polymerase reactions involving transcription/reverse-transcription of modified nucleotides compared with those of natural nucleotides, (ii) technical difficulties and additional time and effort required when using modified nucleic acid libraries, and (iii) ambiguous efficacies of chemical modifications in binding properties until recently; in contrast, the effects of chemical modifications on biostability are well studied using various nucleotide analogs. Although reports on the direct screening of a modified nucleic acid library remain in the minority, chemical modifications would be essential when further functional expansion of nucleic acid aptamers, in particular for medical and biological uses, is considered. This paper focuses on enzymatic production of chemically modified nucleic acids and their application to random screenings. In addition, recent advances and possible future research are also described. |
| format | Article |
| id | doaj-art-46003fd82c584d0c934489dd09ebe4ff |
| institution | Kabale University |
| issn | 2090-0201 2090-021X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-46003fd82c584d0c934489dd09ebe4ff2025-08-20T03:36:14ZengWileyJournal of Nucleic Acids2090-02012090-021X2012-01-01201210.1155/2012/156482156482Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid LibrariesYuuya Kasahara0Masayasu Kuwahara1Graduate School of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu 376-8515, JapanGraduate School of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu 376-8515, JapanSpecific binders comprised of nucleic acids, that is, RNA/DNA aptamers, are attractive functional biopolymers owing to their potential broad application in medicine, food hygiene, environmental analysis, and biological research. Despite the large number of reports on selection of natural DNA/RNA aptamers, there are not many examples of direct screening of chemically modified nucleic acid aptamers. This is because of (i) the inferior efficiency and accuracy of polymerase reactions involving transcription/reverse-transcription of modified nucleotides compared with those of natural nucleotides, (ii) technical difficulties and additional time and effort required when using modified nucleic acid libraries, and (iii) ambiguous efficacies of chemical modifications in binding properties until recently; in contrast, the effects of chemical modifications on biostability are well studied using various nucleotide analogs. Although reports on the direct screening of a modified nucleic acid library remain in the minority, chemical modifications would be essential when further functional expansion of nucleic acid aptamers, in particular for medical and biological uses, is considered. This paper focuses on enzymatic production of chemically modified nucleic acids and their application to random screenings. In addition, recent advances and possible future research are also described.http://dx.doi.org/10.1155/2012/156482 |
| spellingShingle | Yuuya Kasahara Masayasu Kuwahara Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries Journal of Nucleic Acids |
| title | Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries |
| title_full | Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries |
| title_fullStr | Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries |
| title_full_unstemmed | Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries |
| title_short | Artificial Specific Binders Directly Recovered from Chemically Modified Nucleic Acid Libraries |
| title_sort | artificial specific binders directly recovered from chemically modified nucleic acid libraries |
| url | http://dx.doi.org/10.1155/2012/156482 |
| work_keys_str_mv | AT yuuyakasahara artificialspecificbindersdirectlyrecoveredfromchemicallymodifiednucleicacidlibraries AT masayasukuwahara artificialspecificbindersdirectlyrecoveredfromchemicallymodifiednucleicacidlibraries |