Linoleic acid addition prevents Staphylococcus aureus biofilm formation on PMMA bone cement
Acrylic bone cement is widely used in vertebroplasty to treat osteoporosis-induced vertebral compression fractures. However, infection after vertebroplasty is problematic and previous work has suggested loading the bone cement with an antibiotic for prophylaxis. Linoleic acid (LA) has been investiga...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-12-01
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| Series: | Biofilm |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207525000590 |
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| author | Linglu Hong Karin Hjort Dan I. Andersson Cecilia Persson |
| author_facet | Linglu Hong Karin Hjort Dan I. Andersson Cecilia Persson |
| author_sort | Linglu Hong |
| collection | DOAJ |
| description | Acrylic bone cement is widely used in vertebroplasty to treat osteoporosis-induced vertebral compression fractures. However, infection after vertebroplasty is problematic and previous work has suggested loading the bone cement with an antibiotic for prophylaxis. Linoleic acid (LA) has been investigated as a promising additive to improve the mechanical properties of bone cement for vertebroplasty, but LA could potentially also have an antibacterial effect. In this study, we evaluated the antibacterial properties of LA-loaded bone cement by comparing its antibiofilm properties with that of original bone cement through quantification of bacterial growth using viable cell count and scanning electron microscopy. The released monomer (MMA) concentration and the monomer minimum inhibitory concentration were determined to clarify the monomer's potential role in inhibiting bacterial growth. The LA release profile was measured, and a checkerboard assay was done to determine any synergistic effects of LA and the commonly used antibiotic gentamicin. Results show that LA-loaded bone cement could significantly inhibit Staphylococcus aureus biofilm formation, including gentamicin-resistant strains, but with limited effect on Escherichia coli. Furthermore, the released MMA did not have a significant influence on bacterial growth. The checkerboard assay results show that the LA and gentamicin combination could broaden the antibacterial spectrum and increase gentamicin efficacy. In conclusion, LA merits further investigation as an antibacterial agent in bone cement, alone or in combination with antibiotics. |
| format | Article |
| id | doaj-art-45fabe3797ca4b05a2279aed0205dc2b |
| institution | Kabale University |
| issn | 2590-2075 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj-art-45fabe3797ca4b05a2279aed0205dc2b2025-08-20T04:02:32ZengElsevierBiofilm2590-20752025-12-011010031110.1016/j.bioflm.2025.100311Linoleic acid addition prevents Staphylococcus aureus biofilm formation on PMMA bone cementLinglu Hong0Karin Hjort1Dan I. Andersson2Cecilia Persson3Division of Biomedical Engineering, Department of Materials Science and Engineering, Uppsala University, Uppsala, Sweden; Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, SwedenDepartment of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, SwedenDepartment of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, SwedenDivision of Biomedical Engineering, Department of Materials Science and Engineering, Uppsala University, Uppsala, Sweden; Corresponding author.Acrylic bone cement is widely used in vertebroplasty to treat osteoporosis-induced vertebral compression fractures. However, infection after vertebroplasty is problematic and previous work has suggested loading the bone cement with an antibiotic for prophylaxis. Linoleic acid (LA) has been investigated as a promising additive to improve the mechanical properties of bone cement for vertebroplasty, but LA could potentially also have an antibacterial effect. In this study, we evaluated the antibacterial properties of LA-loaded bone cement by comparing its antibiofilm properties with that of original bone cement through quantification of bacterial growth using viable cell count and scanning electron microscopy. The released monomer (MMA) concentration and the monomer minimum inhibitory concentration were determined to clarify the monomer's potential role in inhibiting bacterial growth. The LA release profile was measured, and a checkerboard assay was done to determine any synergistic effects of LA and the commonly used antibiotic gentamicin. Results show that LA-loaded bone cement could significantly inhibit Staphylococcus aureus biofilm formation, including gentamicin-resistant strains, but with limited effect on Escherichia coli. Furthermore, the released MMA did not have a significant influence on bacterial growth. The checkerboard assay results show that the LA and gentamicin combination could broaden the antibacterial spectrum and increase gentamicin efficacy. In conclusion, LA merits further investigation as an antibacterial agent in bone cement, alone or in combination with antibiotics.http://www.sciencedirect.com/science/article/pii/S2590207525000590PMMA bone cementlinoleic acidAntibiofilmMethyl methacrylate monomerAntibiotics |
| spellingShingle | Linglu Hong Karin Hjort Dan I. Andersson Cecilia Persson Linoleic acid addition prevents Staphylococcus aureus biofilm formation on PMMA bone cement Biofilm PMMA bone cement linoleic acid Antibiofilm Methyl methacrylate monomer Antibiotics |
| title | Linoleic acid addition prevents Staphylococcus aureus biofilm formation on PMMA bone cement |
| title_full | Linoleic acid addition prevents Staphylococcus aureus biofilm formation on PMMA bone cement |
| title_fullStr | Linoleic acid addition prevents Staphylococcus aureus biofilm formation on PMMA bone cement |
| title_full_unstemmed | Linoleic acid addition prevents Staphylococcus aureus biofilm formation on PMMA bone cement |
| title_short | Linoleic acid addition prevents Staphylococcus aureus biofilm formation on PMMA bone cement |
| title_sort | linoleic acid addition prevents staphylococcus aureus biofilm formation on pmma bone cement |
| topic | PMMA bone cement linoleic acid Antibiofilm Methyl methacrylate monomer Antibiotics |
| url | http://www.sciencedirect.com/science/article/pii/S2590207525000590 |
| work_keys_str_mv | AT lingluhong linoleicacidadditionpreventsstaphylococcusaureusbiofilmformationonpmmabonecement AT karinhjort linoleicacidadditionpreventsstaphylococcusaureusbiofilmformationonpmmabonecement AT daniandersson linoleicacidadditionpreventsstaphylococcusaureusbiofilmformationonpmmabonecement AT ceciliapersson linoleicacidadditionpreventsstaphylococcusaureusbiofilmformationonpmmabonecement |