Buyang Huanwu decoction promotes angiogenesis and improves hemorheological parameters after cervical spinal cord injury
Objective: To explore the effects of Buyang Huanwu decoction (BYHWD) on vascular neogenesis and hemorheological parameters following cervical spinal cord injury (SCI). Methods: An acute cervical SCI model was established using 84 female Sprague–Dawley rats. Functional recovery of the rats was evalua...
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Elsevier
2024-10-01
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| Series: | Journal of Traditional Chinese Medical Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095754824000644 |
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| author | Luchun Xu Yongdong Yang Guozheng Jiang Yushan Gao Jiawei Song Yukun Ma Jiaojiao Fan Guanlong Wang Xing Yu Xiangsheng Tang |
| author_facet | Luchun Xu Yongdong Yang Guozheng Jiang Yushan Gao Jiawei Song Yukun Ma Jiaojiao Fan Guanlong Wang Xing Yu Xiangsheng Tang |
| author_sort | Luchun Xu |
| collection | DOAJ |
| description | Objective: To explore the effects of Buyang Huanwu decoction (BYHWD) on vascular neogenesis and hemorheological parameters following cervical spinal cord injury (SCI). Methods: An acute cervical SCI model was established using 84 female Sprague–Dawley rats. Functional recovery of the rats was evaluated using the forelimb locomotor scale score, forelimb grip strength test, and Basso-Beattie-Bresnahan score. The animals were subsequently euthanized at days 7 and 28 postoperatively. The gross morphology, neuronal survival, and myelin sheath in the injured area were evaluated using hematoxylin and eosin (HE), Nissl, and luxol fast blue (LFB) staining, respectively. Immunofluorescence staining was used to observe CD31 expression 7 days post-injury. Furthermore, the expression of CD31, neuronal nuclear protein (NeuN), and myelin basic protein (MBP) were evaluated 28 days post-injury. Additionally, vascular endothelial growth factor A (VEGFA) and VEGF receptor-2 (VEGFR-2) expression was evaluated using western blotting. Whole-blood viscosity, plasma viscosity, and red blood cell aggregation were measured using a hemorheometer. Results: From postoperative days 3–28, motor function in the BYHWD group began to recover considerably compared to the SCI group. BYHWD effectively restored spinal cord histopathology. In addition, the number of NeuN-positive cells, and fluorescence intensity of CD31at 7 and 28 days and MBP significantly increased in the BYHWD group compared with the SCI group (all P < .05). Moreover, this decoction significantly upregulated the expression of VEGFA and VEGFR-2 (all P < .05). BYHWD improved the hemorheology results (i.e., except erythrocyte aggregation index in the low-dose group), revealing statistically significant differences compared with the SCI group (all P < .05). Conclusion: BYHWD effectively promoted angiogenesis, improved hemorheological parameters, and protected neurons and myelin sheaths, ultimately promoting the recovery of neurological function after cervical SCI in rats. These findings suggest that BYHWD promotes vascular neogenesis through the VEGFA/VEGFR-2 pathway. |
| format | Article |
| id | doaj-art-45f8543235ec4112acf1bce944dd681c |
| institution | OA Journals |
| issn | 2095-7548 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional Chinese Medical Sciences |
| spelling | doaj-art-45f8543235ec4112acf1bce944dd681c2025-08-20T01:54:15ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482024-10-0111445646510.1016/j.jtcms.2024.08.002Buyang Huanwu decoction promotes angiogenesis and improves hemorheological parameters after cervical spinal cord injuryLuchun Xu0Yongdong Yang1Guozheng Jiang2Yushan Gao3Jiawei Song4Yukun Ma5Jiaojiao Fan6Guanlong Wang7Xing Yu8Xiangsheng Tang9Department of Orthopedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, ChinaDepartment of Orthopedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, ChinaDepartment of Orthopedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, ChinaDepartment of Orthopedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, ChinaDepartment of Orthopedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, ChinaDepartment of Orthopedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, ChinaDepartment of Orthopedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, ChinaDepartment of Orthopedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, China; Corresponding authors.Department of Orthopedics, China-Japan Friendship Hospital, Beijing 100029, China; Corresponding authors.Objective: To explore the effects of Buyang Huanwu decoction (BYHWD) on vascular neogenesis and hemorheological parameters following cervical spinal cord injury (SCI). Methods: An acute cervical SCI model was established using 84 female Sprague–Dawley rats. Functional recovery of the rats was evaluated using the forelimb locomotor scale score, forelimb grip strength test, and Basso-Beattie-Bresnahan score. The animals were subsequently euthanized at days 7 and 28 postoperatively. The gross morphology, neuronal survival, and myelin sheath in the injured area were evaluated using hematoxylin and eosin (HE), Nissl, and luxol fast blue (LFB) staining, respectively. Immunofluorescence staining was used to observe CD31 expression 7 days post-injury. Furthermore, the expression of CD31, neuronal nuclear protein (NeuN), and myelin basic protein (MBP) were evaluated 28 days post-injury. Additionally, vascular endothelial growth factor A (VEGFA) and VEGF receptor-2 (VEGFR-2) expression was evaluated using western blotting. Whole-blood viscosity, plasma viscosity, and red blood cell aggregation were measured using a hemorheometer. Results: From postoperative days 3–28, motor function in the BYHWD group began to recover considerably compared to the SCI group. BYHWD effectively restored spinal cord histopathology. In addition, the number of NeuN-positive cells, and fluorescence intensity of CD31at 7 and 28 days and MBP significantly increased in the BYHWD group compared with the SCI group (all P < .05). Moreover, this decoction significantly upregulated the expression of VEGFA and VEGFR-2 (all P < .05). BYHWD improved the hemorheology results (i.e., except erythrocyte aggregation index in the low-dose group), revealing statistically significant differences compared with the SCI group (all P < .05). Conclusion: BYHWD effectively promoted angiogenesis, improved hemorheological parameters, and protected neurons and myelin sheaths, ultimately promoting the recovery of neurological function after cervical SCI in rats. These findings suggest that BYHWD promotes vascular neogenesis through the VEGFA/VEGFR-2 pathway.http://www.sciencedirect.com/science/article/pii/S2095754824000644Cervical spinal cord injuryBuyang Huanwu decoctionAngiogenesisHemorheologyNeuroprotection |
| spellingShingle | Luchun Xu Yongdong Yang Guozheng Jiang Yushan Gao Jiawei Song Yukun Ma Jiaojiao Fan Guanlong Wang Xing Yu Xiangsheng Tang Buyang Huanwu decoction promotes angiogenesis and improves hemorheological parameters after cervical spinal cord injury Journal of Traditional Chinese Medical Sciences Cervical spinal cord injury Buyang Huanwu decoction Angiogenesis Hemorheology Neuroprotection |
| title | Buyang Huanwu decoction promotes angiogenesis and improves hemorheological parameters after cervical spinal cord injury |
| title_full | Buyang Huanwu decoction promotes angiogenesis and improves hemorheological parameters after cervical spinal cord injury |
| title_fullStr | Buyang Huanwu decoction promotes angiogenesis and improves hemorheological parameters after cervical spinal cord injury |
| title_full_unstemmed | Buyang Huanwu decoction promotes angiogenesis and improves hemorheological parameters after cervical spinal cord injury |
| title_short | Buyang Huanwu decoction promotes angiogenesis and improves hemorheological parameters after cervical spinal cord injury |
| title_sort | buyang huanwu decoction promotes angiogenesis and improves hemorheological parameters after cervical spinal cord injury |
| topic | Cervical spinal cord injury Buyang Huanwu decoction Angiogenesis Hemorheology Neuroprotection |
| url | http://www.sciencedirect.com/science/article/pii/S2095754824000644 |
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