Leptin Administration Downregulates the Increased Expression Levels of Genes Related to Oxidative Stress and Inflammation in the Skeletal Muscle of ob/ob Mice
Obese leptin-deficient ob/ob mice exhibit a low-grade chronic inflammation together with a low muscle mass. Our aim was to analyze the changes in muscle expression levels of genes related to oxidative stress and inflammatory responses in leptin deficiency and to identify the effect of in vivo leptin...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2010/784343 |
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| Summary: | Obese leptin-deficient ob/ob mice exhibit a
low-grade chronic inflammation together with a low muscle mass.
Our aim was to analyze the changes in muscle expression levels of
genes related to oxidative stress and inflammatory responses in
leptin deficiency and to identify the effect of in
vivo leptin administration. Ob/ob mice
were divided in three groups as follows: control
ob/ob, leptin-treated ob/ob
(1 mg/kg/d) and leptin pair-fed ob/ob
mice. Gastrocnemius weight was lower in control
ob/ob than in wild type mice (P<.01) exhibiting an increase after leptin treatment
compared to control and pair-fed (P<.01) ob/ob animals. Thiobarbituric acid
reactive substances, markers of oxidative stress, were higher in
serum (P<.01) and gastrocnemius (P=.05) of control ob/ob than in wild type
mice and were significantly decreased (P<.01) by leptin treatment. Leptin deficiency altered the
expression of 1,546 genes, while leptin treatment modified the
regulation of 1,127 genes with 86 of them being involved in
oxidative stress, immune defense and inflammatory response. Leptin
administration decreased the high expression of Crybb1,
Hspb3, Hspb7, Mt4, Cat, Rbm9, Serpinc1 and
Serpinb1a observed in control
ob/ob mice, indicating that it improves
inflammation and muscle loss. |
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| ISSN: | 0962-9351 1466-1861 |