Transcriptome analysis reveals key regulatory networks and genes involved in the acquisition of cold stress memory in pepper seedlings
Abstract Temperature is an important limiting factor in the counter-seasonal cultivation of pepper. Currently, there are no studies on transcriptomic analysis of ‘cold stress memory’ in pepper. In this study, in order to understand the mechanism of ‘cold stress memory’ in pepper (Capsicum annuum L.)...
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| Language: | English |
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BMC
2024-10-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-05660-x |
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| author | Jian Li Ping Yang Hongbo Fu Juan Li Yanzhuang Wang Keyan Zhu Jihua Yu Jie Li |
| author_facet | Jian Li Ping Yang Hongbo Fu Juan Li Yanzhuang Wang Keyan Zhu Jihua Yu Jie Li |
| author_sort | Jian Li |
| collection | DOAJ |
| description | Abstract Temperature is an important limiting factor in the counter-seasonal cultivation of pepper. Currently, there are no studies on transcriptomic analysis of ‘cold stress memory’ in pepper. In this study, in order to understand the mechanism of ‘cold stress memory’ in pepper (Capsicum annuum L.), seedlings were subjected to the following treatments: normal temperature treatment (P0), the first cold treatment for 3 days (P3), the recovery temperature treatment for 3 days (R3), and another cold treatment for 3 days (RP3). The results showed that P3 plants wilted the most, RP3 the second and R3 the least. Leaf reactive oxygen species (ROS) and electrolyte leakage were the most in P3, the second in RP3 and the least in R3. In addition, RP3 had the highest accumulation of zeaxanthin, violaxanthin and β-cryptoxanthin, followed by P3, and R3 had the least. These results suggest that pepper seedlings are characterized by ‘cold stress memory’. Transcriptomics was used to analyze the key genes and transcription factors involved in the biosynthesis of zeaxanthin, violaxanthin and β-cryptoxanthin during the formation of ‘cold stress memory’. This study provides candidate genes and transcription factors for an in-depth study of the cold tolerance mechanism in pepper. |
| format | Article |
| id | doaj-art-45f2c6134da846c184a69557121cc6cf |
| institution | OA Journals |
| issn | 1471-2229 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-45f2c6134da846c184a69557121cc6cf2025-08-20T02:17:41ZengBMCBMC Plant Biology1471-22292024-10-0124111310.1186/s12870-024-05660-xTranscriptome analysis reveals key regulatory networks and genes involved in the acquisition of cold stress memory in pepper seedlingsJian Li0Ping Yang1Hongbo Fu2Juan Li3Yanzhuang Wang4Keyan Zhu5Jihua Yu6Jie Li7College of Horticulture, Gansu Agriculture UniversityCollege of Biological and Agricultural Sciences, Honghe UniversityCollege of Biological and Agricultural Sciences, Honghe UniversityCollege of Biological and Agricultural Sciences, Honghe UniversityCollege of Horticulture and Forestry, Tarim UniversityCollege of Horticulture, Gansu Agriculture UniversityCollege of Horticulture, Gansu Agriculture UniversityCollege of Biological and Agricultural Sciences, Honghe UniversityAbstract Temperature is an important limiting factor in the counter-seasonal cultivation of pepper. Currently, there are no studies on transcriptomic analysis of ‘cold stress memory’ in pepper. In this study, in order to understand the mechanism of ‘cold stress memory’ in pepper (Capsicum annuum L.), seedlings were subjected to the following treatments: normal temperature treatment (P0), the first cold treatment for 3 days (P3), the recovery temperature treatment for 3 days (R3), and another cold treatment for 3 days (RP3). The results showed that P3 plants wilted the most, RP3 the second and R3 the least. Leaf reactive oxygen species (ROS) and electrolyte leakage were the most in P3, the second in RP3 and the least in R3. In addition, RP3 had the highest accumulation of zeaxanthin, violaxanthin and β-cryptoxanthin, followed by P3, and R3 had the least. These results suggest that pepper seedlings are characterized by ‘cold stress memory’. Transcriptomics was used to analyze the key genes and transcription factors involved in the biosynthesis of zeaxanthin, violaxanthin and β-cryptoxanthin during the formation of ‘cold stress memory’. This study provides candidate genes and transcription factors for an in-depth study of the cold tolerance mechanism in pepper.https://doi.org/10.1186/s12870-024-05660-xCold stress memoryPepperRNA-seqKEGG enrichment |
| spellingShingle | Jian Li Ping Yang Hongbo Fu Juan Li Yanzhuang Wang Keyan Zhu Jihua Yu Jie Li Transcriptome analysis reveals key regulatory networks and genes involved in the acquisition of cold stress memory in pepper seedlings BMC Plant Biology Cold stress memory Pepper RNA-seq KEGG enrichment |
| title | Transcriptome analysis reveals key regulatory networks and genes involved in the acquisition of cold stress memory in pepper seedlings |
| title_full | Transcriptome analysis reveals key regulatory networks and genes involved in the acquisition of cold stress memory in pepper seedlings |
| title_fullStr | Transcriptome analysis reveals key regulatory networks and genes involved in the acquisition of cold stress memory in pepper seedlings |
| title_full_unstemmed | Transcriptome analysis reveals key regulatory networks and genes involved in the acquisition of cold stress memory in pepper seedlings |
| title_short | Transcriptome analysis reveals key regulatory networks and genes involved in the acquisition of cold stress memory in pepper seedlings |
| title_sort | transcriptome analysis reveals key regulatory networks and genes involved in the acquisition of cold stress memory in pepper seedlings |
| topic | Cold stress memory Pepper RNA-seq KEGG enrichment |
| url | https://doi.org/10.1186/s12870-024-05660-x |
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