From Waste to Wealth: Efficient α-Amylase Production Using Agro-Waste Materials: A Study on Brevibacillus Isolates
Soil microbiota serves as a rich source of different microbial enzymes including α-amylase. In this study we aimed to isolate, optimize amylase-producing bacterial strains, and evaluate the effectiveness of agro-waste in replacing conventional starch-based fermentation substrates. Soil samples were...
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ResearchersLinks, Ltd
2025-06-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://researcherslinks.com/current-issues/From-Waste-to-Wealth-Efficient-Amylase-Production/44/1/11293/html |
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| author | Fabiha Shahid Syeda Soran Alam Aima Arooj Hafsa Batool |
| author_facet | Fabiha Shahid Syeda Soran Alam Aima Arooj Hafsa Batool |
| author_sort | Fabiha Shahid |
| collection | DOAJ |
| description | Soil microbiota serves as a rich source of different microbial enzymes including α-amylase. In this study we aimed to isolate, optimize amylase-producing bacterial strains, and evaluate the effectiveness of agro-waste in replacing conventional starch-based fermentation substrates. Soil samples were collected from different areas of Pakistan. Bacteria that produce α-amylase were isolated and characterized. Initial screening in vitro was performed by starch hydrolysis assay. This process resulted in the identification of two promising bacterial isolates, AU1 and AU2, which were further characterized through biochemical testing and 16S rRNA sequencing. They were identified as Brevibacillus reuszeri and Brevibacillus brevis, respectively. Optimization of the fermentation conditions revealed optimal pH 7, temperature of 38 °C, incubation period of 72 h, and yeast extract as an organic nitrogen source. Additionally, using different agro-waste, general waste, and several sources of starch including corn comb, wheat bran, rice bran, potato peel, rice starch, potato starch, and corn starch, Brevibacillus reuszeri, and Brevibacillus brevis were tested for the production of α-amylase. The maximum amylase production was observed by Brevibacillus reuszeri with wheat bran (381 μmol/ min.) and Brevibacillus brevis with potato peel (395 μmol/ min.). Our research provides significant insights into the optimization of the fermentation processes for α-amylase production, highlighting the economic and sustainable potential of utilizing agro-waste materials in enzyme production industries. |
| format | Article |
| id | doaj-art-45e8dff90d0a468e8bb17c4eb0ea39ac |
| institution | Kabale University |
| issn | 2537-0286 2537-0294 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | ResearchersLinks, Ltd |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj-art-45e8dff90d0a468e8bb17c4eb0ea39ac2025-08-20T03:45:02ZengResearchersLinks, LtdNovel Research in Microbiology Journal2537-02862537-02942025-06-0193166177https://dx.doi.org/10.17582/journal.NRMJ/2025/9.3.166.177From Waste to Wealth: Efficient α-Amylase Production Using Agro-Waste Materials: A Study on Brevibacillus IsolatesFabiha Shahid0Syeda Soran Alam1Aima Arooj 2Hafsa Batool3Institute of Industrial Biotechnology Department, Government College University Lahore, 54000, Pakistan.Institute of Industrial Biotechnology Department, Government College University Lahore, 54000, Pakistan.Institute of Industrial Biotechnology Department, Government College University Lahore, 54000, Pakistan.Institute of Industrial Biotechnology Department, Government College University Lahore, 54000, Pakistan.Soil microbiota serves as a rich source of different microbial enzymes including α-amylase. In this study we aimed to isolate, optimize amylase-producing bacterial strains, and evaluate the effectiveness of agro-waste in replacing conventional starch-based fermentation substrates. Soil samples were collected from different areas of Pakistan. Bacteria that produce α-amylase were isolated and characterized. Initial screening in vitro was performed by starch hydrolysis assay. This process resulted in the identification of two promising bacterial isolates, AU1 and AU2, which were further characterized through biochemical testing and 16S rRNA sequencing. They were identified as Brevibacillus reuszeri and Brevibacillus brevis, respectively. Optimization of the fermentation conditions revealed optimal pH 7, temperature of 38 °C, incubation period of 72 h, and yeast extract as an organic nitrogen source. Additionally, using different agro-waste, general waste, and several sources of starch including corn comb, wheat bran, rice bran, potato peel, rice starch, potato starch, and corn starch, Brevibacillus reuszeri, and Brevibacillus brevis were tested for the production of α-amylase. The maximum amylase production was observed by Brevibacillus reuszeri with wheat bran (381 μmol/ min.) and Brevibacillus brevis with potato peel (395 μmol/ min.). Our research provides significant insights into the optimization of the fermentation processes for α-amylase production, highlighting the economic and sustainable potential of utilizing agro-waste materials in enzyme production industries.https://researcherslinks.com/current-issues/From-Waste-to-Wealth-Efficient-Amylase-Production/44/1/11293/htmlagro-wasteamylasestarch hydrolysis assayeconomic substratesoptimizationyeast extract |
| spellingShingle | Fabiha Shahid Syeda Soran Alam Aima Arooj Hafsa Batool From Waste to Wealth: Efficient α-Amylase Production Using Agro-Waste Materials: A Study on Brevibacillus Isolates Novel Research in Microbiology Journal agro-waste amylase starch hydrolysis assay economic substrates optimization yeast extract |
| title | From Waste to Wealth: Efficient α-Amylase Production Using Agro-Waste Materials: A Study on Brevibacillus Isolates |
| title_full | From Waste to Wealth: Efficient α-Amylase Production Using Agro-Waste Materials: A Study on Brevibacillus Isolates |
| title_fullStr | From Waste to Wealth: Efficient α-Amylase Production Using Agro-Waste Materials: A Study on Brevibacillus Isolates |
| title_full_unstemmed | From Waste to Wealth: Efficient α-Amylase Production Using Agro-Waste Materials: A Study on Brevibacillus Isolates |
| title_short | From Waste to Wealth: Efficient α-Amylase Production Using Agro-Waste Materials: A Study on Brevibacillus Isolates |
| title_sort | from waste to wealth efficient α amylase production using agro waste materials a study on brevibacillus isolates |
| topic | agro-waste amylase starch hydrolysis assay economic substrates optimization yeast extract |
| url | https://researcherslinks.com/current-issues/From-Waste-to-Wealth-Efficient-Amylase-Production/44/1/11293/html |
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