Heterogeneity of virulence factors among <i>Porphyromonas gingivalis</i> clinical isolates from patients with chronic generalized periodontitis
The development of chronic generalized periodontitis occurs due to a combination of a several causes, among which the leading role is assigned to periodontal pathogens, which include P. gingivalis. Among P. gingivalis virulence factors, the polysaccharide capsule, fimbria proteins, cysteine protease...
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Sankt-Peterburg : NIIÈM imeni Pastera
2024-12-01
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| Series: | Инфекция и иммунитет |
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| Online Access: | https://iimmun.ru/iimm/article/viewFile/17783/2093 |
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| author | I. V. Koroleva Ekaterina S. Mikhailova K. A. Privalova L. A. Ermolaeva S. A. Tumanova A. N. Suvorov |
| author_facet | I. V. Koroleva Ekaterina S. Mikhailova K. A. Privalova L. A. Ermolaeva S. A. Tumanova A. N. Suvorov |
| author_sort | I. V. Koroleva |
| collection | DOAJ |
| description | The development of chronic generalized periodontitis occurs due to a combination of a several causes, among which the leading role is assigned to periodontal pathogens, which include P. gingivalis. Among P. gingivalis virulence factors, the polysaccharide capsule, fimbria proteins, cysteine proteases, and hemagglutinins are of special importance. The study was aimed to investigate the prevalence of specific virulence genes and identify a virulent genotype among P. gingivalis isolates found in patients with severe chronic generalized periodontitis (CGP). 41 patients (27 women and 14 men, average age 43.9±1.5 years) were examined, of which main and control group consisted of 22 patients with severe CGP and 19 patients without inflammatory periodontal diseases, respectively. The PCR data allow to consider type II fimbria (FimA II), arginine-dependent type A protease (RghA) and lysine-dependent protease (Kgh) as specific markers for the detection of more virulent P. gingivalis strains. It was found that in St. Petersburg, the following P. gingivalis genotypes predominate among patients with severe CGP: fimA II:kg:rghA, fimA II:kgh and fimA II:rghA. In addition, it has been demonstrated that virulent genotypes are detected to a small extent in P. gingivalis isolates from healthy control group patients. The identification of P. gingivalis strains with a more prominent pathogenic potential and the detection of their virulent genotypes is of great practical importance, in the future allowing to develop advanced effective methods for disease prevention to be used in a personalized medicine strategy. The results obtained are also of high importance due to the recorded variability in the circulation of P. gingivalis strain genotypes in various worldwide regions. |
| format | Article |
| id | doaj-art-45e7201207e146f1adeaa4b65faabebc |
| institution | DOAJ |
| issn | 2220-7619 2313-7398 |
| language | Russian |
| publishDate | 2024-12-01 |
| publisher | Sankt-Peterburg : NIIÈM imeni Pastera |
| record_format | Article |
| series | Инфекция и иммунитет |
| spelling | doaj-art-45e7201207e146f1adeaa4b65faabebc2025-08-20T02:59:53ZrusSankt-Peterburg : NIIÈM imeni PasteraИнфекция и иммунитет2220-76192313-73982024-12-011461079108610.15789/2220-7619-HOV-177831478Heterogeneity of virulence factors among <i>Porphyromonas gingivalis</i> clinical isolates from patients with chronic generalized periodontitisI. V. Koroleva0Ekaterina S. Mikhailova1K. A. Privalova2L. A. Ermolaeva3S. A. Tumanova4A. N. Suvorov5St. Petersburg State UniversitySt. Petersburg State UniversityPavlov First St. Petersburg State Medical UniversitySt. Petersburg State UniversitySt. Petersburg State UniversitySt. Petersburg State UniversityThe development of chronic generalized periodontitis occurs due to a combination of a several causes, among which the leading role is assigned to periodontal pathogens, which include P. gingivalis. Among P. gingivalis virulence factors, the polysaccharide capsule, fimbria proteins, cysteine proteases, and hemagglutinins are of special importance. The study was aimed to investigate the prevalence of specific virulence genes and identify a virulent genotype among P. gingivalis isolates found in patients with severe chronic generalized periodontitis (CGP). 41 patients (27 women and 14 men, average age 43.9±1.5 years) were examined, of which main and control group consisted of 22 patients with severe CGP and 19 patients without inflammatory periodontal diseases, respectively. The PCR data allow to consider type II fimbria (FimA II), arginine-dependent type A protease (RghA) and lysine-dependent protease (Kgh) as specific markers for the detection of more virulent P. gingivalis strains. It was found that in St. Petersburg, the following P. gingivalis genotypes predominate among patients with severe CGP: fimA II:kg:rghA, fimA II:kgh and fimA II:rghA. In addition, it has been demonstrated that virulent genotypes are detected to a small extent in P. gingivalis isolates from healthy control group patients. The identification of P. gingivalis strains with a more prominent pathogenic potential and the detection of their virulent genotypes is of great practical importance, in the future allowing to develop advanced effective methods for disease prevention to be used in a personalized medicine strategy. The results obtained are also of high importance due to the recorded variability in the circulation of P. gingivalis strain genotypes in various worldwide regions.https://iimmun.ru/iimm/article/viewFile/17783/2093chronic generalized periodontitisheterogeneityvirulent genotypep. gingivalisfimbriaecysteine proteases |
| spellingShingle | I. V. Koroleva Ekaterina S. Mikhailova K. A. Privalova L. A. Ermolaeva S. A. Tumanova A. N. Suvorov Heterogeneity of virulence factors among <i>Porphyromonas gingivalis</i> clinical isolates from patients with chronic generalized periodontitis Инфекция и иммунитет chronic generalized periodontitis heterogeneity virulent genotype p. gingivalis fimbriae cysteine proteases |
| title | Heterogeneity of virulence factors among <i>Porphyromonas gingivalis</i> clinical isolates from patients with chronic generalized periodontitis |
| title_full | Heterogeneity of virulence factors among <i>Porphyromonas gingivalis</i> clinical isolates from patients with chronic generalized periodontitis |
| title_fullStr | Heterogeneity of virulence factors among <i>Porphyromonas gingivalis</i> clinical isolates from patients with chronic generalized periodontitis |
| title_full_unstemmed | Heterogeneity of virulence factors among <i>Porphyromonas gingivalis</i> clinical isolates from patients with chronic generalized periodontitis |
| title_short | Heterogeneity of virulence factors among <i>Porphyromonas gingivalis</i> clinical isolates from patients with chronic generalized periodontitis |
| title_sort | heterogeneity of virulence factors among i porphyromonas gingivalis i clinical isolates from patients with chronic generalized periodontitis |
| topic | chronic generalized periodontitis heterogeneity virulent genotype p. gingivalis fimbriae cysteine proteases |
| url | https://iimmun.ru/iimm/article/viewFile/17783/2093 |
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