Effects of Different Doses of Quercetin on Apoptotic Markers and Liver Enzymes in Liver Injury Induced by Interferon Beta-1b (Betaferon) in Male Rats in Comparison with Silymarin
The first disease-modifying drug for the treatment of multiple sclerosis (MS) was interferon beta 1b (IFN).this drug demonstrated efficacy in relapse MS, But There are many difficulties in treating multiple sclerosis, including the well-known side effect of betaferon(hepatotoxicity or liver injury)...
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College of Pharmacy University of Baghdad
2025-06-01
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| Series: | Iraqi Journal of Pharmaceutical Sciences |
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| author | Noor Kamel Obead Inam Sameh Arif Huda jaber waheed |
| author_facet | Noor Kamel Obead Inam Sameh Arif Huda jaber waheed |
| author_sort | Noor Kamel Obead |
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The first disease-modifying drug for the treatment of multiple sclerosis (MS) was interferon beta 1b (IFN).this drug demonstrated efficacy in relapse MS, But There are many difficulties in treating multiple sclerosis, including the well-known side effect of betaferon(hepatotoxicity or liver injury), which present as brief, mild elevations in serum aminotransferase (ALT,AST) levels appear 3 to 12 months after starting treatment. A naturally occurring antioxidant is quercetin which act as anti-oxidant, anti-inflammatory agent , it also have antiviral, anticancer and cardiovascular properties.The study aimed to investigate the apoptotic potential of betaferon on liver cells and to evaluate the hepatoprotective effect of the antioxidant quercetin , by downregulation of apoptotic biomarkers caspase 3 and caspase 9.The study was employed on 36 male rats for 17 days. rats were randomly divided into six groups (6 rats in each group) given daily doses given by Intraperitoneal route (i.p)as follow:Control group; (rats were administered distilled water),betaferon group or induction group ; (rats were administered Betaferon in a dose of 250 µ/kg for 16 days) ,treatment groups betaferon+ quercetin which divided into three subgroups according to the dose of quercetin (25,50,100mg/kg) ; (Rats were pretreated by a dose of 25,50,100mg/kg of quercetin suspension for 6 days then continue administering the same dose of quercetin plus betaferon 250µ/kg on day 7 for 10 days) .and the last group was Silymarin group; (rats were treated with 100 mg/kg of Silymarin suspension for 16 days).In the current study liver function enzymes such as alanine aminotransferase and aspartate aminotransferase also apoptotic markers which include caspase3 and caspase9 were measured.Betaferon significantly increased liver apoptotic markers caspase3 and caspase 9 also cause elevation in the liver enzymes(ALT,AST)compared to the control group. On the other side, quercetin significantly reduce these biomarkers(caspase 3,caspase 9)also restoring liver enzymes to its normal level. Quercetin showed antioxidant,anti_inflammatory and anti_apoptotic effects on the liver tissue of rats that were pretreated with querctin before receiving the betaferon,suggesting it has a beneficial effects on minimizing liver injury or hepatotoxicity caused by betaferon.
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| format | Article |
| id | doaj-art-45e04dcf06f8451f8b83a4e6ef5adf46 |
| institution | DOAJ |
| issn | 1683-3597 2521-3512 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | College of Pharmacy University of Baghdad |
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| series | Iraqi Journal of Pharmaceutical Sciences |
| spelling | doaj-art-45e04dcf06f8451f8b83a4e6ef5adf462025-08-20T03:24:02ZengCollege of Pharmacy University of BaghdadIraqi Journal of Pharmaceutical Sciences1683-35972521-35122025-06-0134210.31351/vol34iss2pp108-115Effects of Different Doses of Quercetin on Apoptotic Markers and Liver Enzymes in Liver Injury Induced by Interferon Beta-1b (Betaferon) in Male Rats in Comparison with SilymarinNoor Kamel Obead0Inam Sameh Arif1Huda jaber waheed2Department of Pharmacology and Toxicology , College of Pharmacy, Mustansiriyah University, Baghdad, IraqDepartment of Pharmacology and Toxicology , College of Pharmacy, Mustansiriyah University, Baghdad, IraqDepartment of Pharmacology and Toxicology , College of Pharmacy, Mustansiriyah University, Baghdad, Iraq The first disease-modifying drug for the treatment of multiple sclerosis (MS) was interferon beta 1b (IFN).this drug demonstrated efficacy in relapse MS, But There are many difficulties in treating multiple sclerosis, including the well-known side effect of betaferon(hepatotoxicity or liver injury), which present as brief, mild elevations in serum aminotransferase (ALT,AST) levels appear 3 to 12 months after starting treatment. A naturally occurring antioxidant is quercetin which act as anti-oxidant, anti-inflammatory agent , it also have antiviral, anticancer and cardiovascular properties.The study aimed to investigate the apoptotic potential of betaferon on liver cells and to evaluate the hepatoprotective effect of the antioxidant quercetin , by downregulation of apoptotic biomarkers caspase 3 and caspase 9.The study was employed on 36 male rats for 17 days. rats were randomly divided into six groups (6 rats in each group) given daily doses given by Intraperitoneal route (i.p)as follow:Control group; (rats were administered distilled water),betaferon group or induction group ; (rats were administered Betaferon in a dose of 250 µ/kg for 16 days) ,treatment groups betaferon+ quercetin which divided into three subgroups according to the dose of quercetin (25,50,100mg/kg) ; (Rats were pretreated by a dose of 25,50,100mg/kg of quercetin suspension for 6 days then continue administering the same dose of quercetin plus betaferon 250µ/kg on day 7 for 10 days) .and the last group was Silymarin group; (rats were treated with 100 mg/kg of Silymarin suspension for 16 days).In the current study liver function enzymes such as alanine aminotransferase and aspartate aminotransferase also apoptotic markers which include caspase3 and caspase9 were measured.Betaferon significantly increased liver apoptotic markers caspase3 and caspase 9 also cause elevation in the liver enzymes(ALT,AST)compared to the control group. On the other side, quercetin significantly reduce these biomarkers(caspase 3,caspase 9)also restoring liver enzymes to its normal level. Quercetin showed antioxidant,anti_inflammatory and anti_apoptotic effects on the liver tissue of rats that were pretreated with querctin before receiving the betaferon,suggesting it has a beneficial effects on minimizing liver injury or hepatotoxicity caused by betaferon. https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/3099Drug induced liver injury, Interferon beta 1b(betaferon), Liver injury, Multiple sclerosis, Quercetin. |
| spellingShingle | Noor Kamel Obead Inam Sameh Arif Huda jaber waheed Effects of Different Doses of Quercetin on Apoptotic Markers and Liver Enzymes in Liver Injury Induced by Interferon Beta-1b (Betaferon) in Male Rats in Comparison with Silymarin Iraqi Journal of Pharmaceutical Sciences Drug induced liver injury, Interferon beta 1b(betaferon), Liver injury, Multiple sclerosis, Quercetin. |
| title | Effects of Different Doses of Quercetin on Apoptotic Markers and Liver Enzymes in Liver Injury Induced by Interferon Beta-1b (Betaferon) in Male Rats in Comparison with Silymarin |
| title_full | Effects of Different Doses of Quercetin on Apoptotic Markers and Liver Enzymes in Liver Injury Induced by Interferon Beta-1b (Betaferon) in Male Rats in Comparison with Silymarin |
| title_fullStr | Effects of Different Doses of Quercetin on Apoptotic Markers and Liver Enzymes in Liver Injury Induced by Interferon Beta-1b (Betaferon) in Male Rats in Comparison with Silymarin |
| title_full_unstemmed | Effects of Different Doses of Quercetin on Apoptotic Markers and Liver Enzymes in Liver Injury Induced by Interferon Beta-1b (Betaferon) in Male Rats in Comparison with Silymarin |
| title_short | Effects of Different Doses of Quercetin on Apoptotic Markers and Liver Enzymes in Liver Injury Induced by Interferon Beta-1b (Betaferon) in Male Rats in Comparison with Silymarin |
| title_sort | effects of different doses of quercetin on apoptotic markers and liver enzymes in liver injury induced by interferon beta 1b betaferon in male rats in comparison with silymarin |
| topic | Drug induced liver injury, Interferon beta 1b(betaferon), Liver injury, Multiple sclerosis, Quercetin. |
| url | https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/3099 |
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