Blood immune profiling of Ethiopian patients with breast cancer highlights different forms of immune escape
Breast cancer (BC) is a leading cause of death worldwide, particularly also among African woman. In order to better stratify patients for the most effective (immuno-) therapy, an in depth characterization of the immune status of BC patients is required. In this study, a cohort of 65 Ethiopian patien...
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Taylor & Francis Group
2024-12-01
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| Series: | OncoImmunology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/2162402X.2024.2436227 |
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| author | Meron Yohannes Chiara Massa Zelalem Desalegn Kathrin Stückrath Anja Mueller Endale Anberber Yonas Bekuretsion Mathewos Assefa Pablo Santos Adamu Addissie Marcus Bauer Claudia Wickenhauser Lesley Taylor Martina Vetter Eva Johanna Kantelhardt Tamrat Abebe Barbara Seliger |
| author_facet | Meron Yohannes Chiara Massa Zelalem Desalegn Kathrin Stückrath Anja Mueller Endale Anberber Yonas Bekuretsion Mathewos Assefa Pablo Santos Adamu Addissie Marcus Bauer Claudia Wickenhauser Lesley Taylor Martina Vetter Eva Johanna Kantelhardt Tamrat Abebe Barbara Seliger |
| author_sort | Meron Yohannes |
| collection | DOAJ |
| description | Breast cancer (BC) is a leading cause of death worldwide, particularly also among African woman. In order to better stratify patients for the most effective (immuno-) therapy, an in depth characterization of the immune status of BC patients is required. In this study, a cohort of 65 Ethiopian patients with primary BC underwent immune profiling by multicolor flow cytometry on peripheral blood samples collected prior to surgery and to any other therapy. Comparison with peripheral blood samples from healthy donors highlighted a general activation of the immune system, accompanied by the presence of exhausted CD4+ T cells and senescent CD8+ T cells with an inverted CD4/CD8 ratio in approximately 50% of BC cases. Enhanced frequencies of γδ T cells, myeloid-derived suppressor cells and regulatory T cells were also found. Correlation with clinical parameters demonstrated a progressive reduction in T cell frequencies with increasing histopathological grading of the tumor. Differences in CD8+ T cells and B cells were also noted among luminal and non-luminal BC subtypes. In conclusion, Ethiopian BC patients showed several alterations in the composition and activation status of the blood immune cell repertoire, which were phenotypically associated with immune suppression. The role of these immunological changes in the clinical outcome of patients with BC will have to be determined in follow-up studies and confirmed in additional patients’ cohorts. |
| format | Article |
| id | doaj-art-45dbd50e44934629a48b0b7f0fa9761a |
| institution | DOAJ |
| issn | 2162-402X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | OncoImmunology |
| spelling | doaj-art-45dbd50e44934629a48b0b7f0fa9761a2025-08-20T02:50:37ZengTaylor & Francis GroupOncoImmunology2162-402X2024-12-0113110.1080/2162402X.2024.2436227Blood immune profiling of Ethiopian patients with breast cancer highlights different forms of immune escapeMeron Yohannes0Chiara Massa1Zelalem Desalegn2Kathrin Stückrath3Anja Mueller4Endale Anberber5Yonas Bekuretsion6Mathewos Assefa7Pablo Santos8Adamu Addissie9Marcus Bauer10Claudia Wickenhauser11Lesley Taylor12Martina Vetter13Eva Johanna Kantelhardt14Tamrat Abebe15Barbara Seliger16Department of Microbiology, Immunology & Parasitology, School of Medicine, Addis Ababa University, Addis Ababa, EthiopiaInstitute of Translational Immunology, Brandenburg Medical School “Theodor Fontane”, Brandenburg an der Havel, GermanyDepartment of Microbiology, Immunology & Parasitology, School of Medicine, Addis Ababa University, Addis Ababa, EthiopiaUniversity Clinic and Polyclinic for Gynecology, Martin Luther University Halle-Wittenberg, Halle (Saale), GermanyInstitute of Medical Immunology, Medical Faculty, Martin Luther University Halle-Wittenberg, Halle (Saale), GermanyDepartment of Surgery, School of Medicine, Addis Ababa University, Addis Ababa, EthiopiaDepartment of Pathology, School of Medicine, Addis Ababa University, Addis Ababa, EthiopiaDepartment of Oncology, School of Medicine, Addis Ababa University, Addis Ababa, EthiopiaGlobal and Planetary Health Working Group, Institute of Medical Epidemiology, Biometrics and Informatics, Martin Luther University of Halle-Wittenberg, Halle (Saale), GermanyGlobal and Planetary Health Working Group, Institute of Medical Epidemiology, Biometrics and Informatics, Martin Luther University of Halle-Wittenberg, Halle (Saale), GermanyGlobal and Planetary Health Working Group, Institute of Medical Epidemiology, Biometrics and Informatics, Martin Luther University of Halle-Wittenberg, Halle (Saale), GermanyInstitute of Pathology, Martin Luther University Halle-Wittenberg, Halle (Saale), GermanyCity of Hope National Medical Center, Duarte, CA, USAGlobal and Planetary Health Working Group, Institute of Medical Epidemiology, Biometrics and Informatics, Martin Luther University of Halle-Wittenberg, Halle (Saale), GermanyGlobal and Planetary Health Working Group, Institute of Medical Epidemiology, Biometrics and Informatics, Martin Luther University of Halle-Wittenberg, Halle (Saale), GermanyDepartment of Microbiology, Immunology & Parasitology, School of Medicine, Addis Ababa University, Addis Ababa, EthiopiaInstitute of Translational Immunology, Brandenburg Medical School “Theodor Fontane”, Brandenburg an der Havel, GermanyBreast cancer (BC) is a leading cause of death worldwide, particularly also among African woman. In order to better stratify patients for the most effective (immuno-) therapy, an in depth characterization of the immune status of BC patients is required. In this study, a cohort of 65 Ethiopian patients with primary BC underwent immune profiling by multicolor flow cytometry on peripheral blood samples collected prior to surgery and to any other therapy. Comparison with peripheral blood samples from healthy donors highlighted a general activation of the immune system, accompanied by the presence of exhausted CD4+ T cells and senescent CD8+ T cells with an inverted CD4/CD8 ratio in approximately 50% of BC cases. Enhanced frequencies of γδ T cells, myeloid-derived suppressor cells and regulatory T cells were also found. Correlation with clinical parameters demonstrated a progressive reduction in T cell frequencies with increasing histopathological grading of the tumor. Differences in CD8+ T cells and B cells were also noted among luminal and non-luminal BC subtypes. In conclusion, Ethiopian BC patients showed several alterations in the composition and activation status of the blood immune cell repertoire, which were phenotypically associated with immune suppression. The role of these immunological changes in the clinical outcome of patients with BC will have to be determined in follow-up studies and confirmed in additional patients’ cohorts.https://www.tandfonline.com/doi/10.1080/2162402X.2024.2436227Biomarkerbloodbreast cancerimmune escape |
| spellingShingle | Meron Yohannes Chiara Massa Zelalem Desalegn Kathrin Stückrath Anja Mueller Endale Anberber Yonas Bekuretsion Mathewos Assefa Pablo Santos Adamu Addissie Marcus Bauer Claudia Wickenhauser Lesley Taylor Martina Vetter Eva Johanna Kantelhardt Tamrat Abebe Barbara Seliger Blood immune profiling of Ethiopian patients with breast cancer highlights different forms of immune escape OncoImmunology Biomarker blood breast cancer immune escape |
| title | Blood immune profiling of Ethiopian patients with breast cancer highlights different forms of immune escape |
| title_full | Blood immune profiling of Ethiopian patients with breast cancer highlights different forms of immune escape |
| title_fullStr | Blood immune profiling of Ethiopian patients with breast cancer highlights different forms of immune escape |
| title_full_unstemmed | Blood immune profiling of Ethiopian patients with breast cancer highlights different forms of immune escape |
| title_short | Blood immune profiling of Ethiopian patients with breast cancer highlights different forms of immune escape |
| title_sort | blood immune profiling of ethiopian patients with breast cancer highlights different forms of immune escape |
| topic | Biomarker blood breast cancer immune escape |
| url | https://www.tandfonline.com/doi/10.1080/2162402X.2024.2436227 |
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