PET Imaging of CD206 Macrophages in Bleomycin-Induced Lung Injury Mouse Model
<b>Background/Objectives</b>: The identification of inflammatory mediators and the involvement of CD206 macrophages in anti-inflammatory responses, along with the synthesis of fibrotic mediators, are crucial for the diagnosis and treatment of Idiopathic Pulmonary Fibrosis (IPF). <b>...
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2025-02-01
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| Online Access: | https://www.mdpi.com/1999-4923/17/2/253 |
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| author | Volkan Tekin Yujun Zhang Clayton Yates Jesse Jaynes Henry Lopez Charles Garvin Benjamin M. Larimer Suzanne E. Lapi |
| author_facet | Volkan Tekin Yujun Zhang Clayton Yates Jesse Jaynes Henry Lopez Charles Garvin Benjamin M. Larimer Suzanne E. Lapi |
| author_sort | Volkan Tekin |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: The identification of inflammatory mediators and the involvement of CD206 macrophages in anti-inflammatory responses, along with the synthesis of fibrotic mediators, are crucial for the diagnosis and treatment of Idiopathic Pulmonary Fibrosis (IPF). <b>Methods</b>: In this study, the assessment of <sup>68</sup>Ga-labeled linear and cyclic forms of the RP832c peptide, which demonstrate a specific affinity for CD206 macrophages, was performed to evaluate efficacy for CD206 imaging through PET/CT, biodistribution studies, and CD206 staining in a bleomycin-induced lung injury mouse model (BLM). This model serves as a representative framework for inflammation and fibrosis. <b>Results</b>: The findings reveal significant peak PET/CT signals (SUV means), ID/gram values, and CD206 staining scores in lung tissues at one week post bleomycin instillation, likely due to the heightened expression of CD206 in the bleomycin-induced lung injury model. In contrast, the healthy mice exhibited no detectable CD206 staining, lower PET signals, and reduced radiopharmaceutical accumulation in lung tissues at the same timepoint. <b>Conclusions</b>: These findings suggest that both linear and cyclic [<sup>68</sup>Ga]Ga-RP832c may function as promising PET imaging agents for CD206 macrophages, and thereby a strategy to non-invasively explore the role of macrophages during fibrogenesis. |
| format | Article |
| id | doaj-art-45da3272d7644bb08de8c729e23dd524 |
| institution | DOAJ |
| issn | 1999-4923 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj-art-45da3272d7644bb08de8c729e23dd5242025-08-20T03:12:19ZengMDPI AGPharmaceutics1999-49232025-02-0117225310.3390/pharmaceutics17020253PET Imaging of CD206 Macrophages in Bleomycin-Induced Lung Injury Mouse ModelVolkan Tekin0Yujun Zhang1Clayton Yates2Jesse Jaynes3Henry Lopez4Charles Garvin5Benjamin M. Larimer6Suzanne E. Lapi7Department of Radiology, University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Radiology, University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Pathology, Johns Hopkins School of Medicine, Baltimore, MD 21287-0013, USADepartment of Biology and Center for Cancer Research, Tuskegee University, Tuskegee, AL 36088, USAMuriGenics, Vallejo, CA 94592, USARiptide Bioscience, Vallejo, CA 94592, USADepartment of Radiology, University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Radiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA<b>Background/Objectives</b>: The identification of inflammatory mediators and the involvement of CD206 macrophages in anti-inflammatory responses, along with the synthesis of fibrotic mediators, are crucial for the diagnosis and treatment of Idiopathic Pulmonary Fibrosis (IPF). <b>Methods</b>: In this study, the assessment of <sup>68</sup>Ga-labeled linear and cyclic forms of the RP832c peptide, which demonstrate a specific affinity for CD206 macrophages, was performed to evaluate efficacy for CD206 imaging through PET/CT, biodistribution studies, and CD206 staining in a bleomycin-induced lung injury mouse model (BLM). This model serves as a representative framework for inflammation and fibrosis. <b>Results</b>: The findings reveal significant peak PET/CT signals (SUV means), ID/gram values, and CD206 staining scores in lung tissues at one week post bleomycin instillation, likely due to the heightened expression of CD206 in the bleomycin-induced lung injury model. In contrast, the healthy mice exhibited no detectable CD206 staining, lower PET signals, and reduced radiopharmaceutical accumulation in lung tissues at the same timepoint. <b>Conclusions</b>: These findings suggest that both linear and cyclic [<sup>68</sup>Ga]Ga-RP832c may function as promising PET imaging agents for CD206 macrophages, and thereby a strategy to non-invasively explore the role of macrophages during fibrogenesis.https://www.mdpi.com/1999-4923/17/2/253idiopathic pulmonary fibrosisbleomycinmacrophagesCD206PET imaging |
| spellingShingle | Volkan Tekin Yujun Zhang Clayton Yates Jesse Jaynes Henry Lopez Charles Garvin Benjamin M. Larimer Suzanne E. Lapi PET Imaging of CD206 Macrophages in Bleomycin-Induced Lung Injury Mouse Model Pharmaceutics idiopathic pulmonary fibrosis bleomycin macrophages CD206 PET imaging |
| title | PET Imaging of CD206 Macrophages in Bleomycin-Induced Lung Injury Mouse Model |
| title_full | PET Imaging of CD206 Macrophages in Bleomycin-Induced Lung Injury Mouse Model |
| title_fullStr | PET Imaging of CD206 Macrophages in Bleomycin-Induced Lung Injury Mouse Model |
| title_full_unstemmed | PET Imaging of CD206 Macrophages in Bleomycin-Induced Lung Injury Mouse Model |
| title_short | PET Imaging of CD206 Macrophages in Bleomycin-Induced Lung Injury Mouse Model |
| title_sort | pet imaging of cd206 macrophages in bleomycin induced lung injury mouse model |
| topic | idiopathic pulmonary fibrosis bleomycin macrophages CD206 PET imaging |
| url | https://www.mdpi.com/1999-4923/17/2/253 |
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