Fine Mapping of <i>BrTCP1</i> as a Key Regulator of Branching in Flowering Chinese Cabbage (<i>Brassica rapa</i> subsp. <i>chinensis</i>)

Fine Mapping of <i>BrTCP1</i> as a Key Regulator of Branching in Flowering Chinese Cabbage (<i>Brassica rapa</i> subsp. <i>chinensis</i>)

Branching is a critical agronomic trait in flowering Chinese cabbage (<i>Brassica rapa</i> subsp. <i>chinensis</i>), influencing plant architecture and yield. In this study, there was a highly significant difference between CX010 (single primary rosette branches) and BCT18 (m...

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Bibliographic Details
Main Authors: Chuanhong Liu, Xinghua Qi, Shuo Fu, Chao Zheng, Chao Wu, Xiaoyu Li, Yun Zhang, Xueling Ye
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Horticulturae
Subjects:
flowering Chinese cabbage
primary rosette branches
QTL mapping
<i>BrTCP1</i>
heterologous complementation
Online Access:https://www.mdpi.com/2311-7524/11/7/824
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Summary:Branching is a critical agronomic trait in flowering Chinese cabbage (<i>Brassica rapa</i> subsp. <i>chinensis</i>), influencing plant architecture and yield. In this study, there was a highly significant difference between CX010 (single primary rosette branches) and BCT18 (multiple primary rosette branches). Phenotypic analysis revealed significant differences in primary rosette branch numbers, with BCT18 showing up to 15 branches and CX010 displaying only one main stem branch. Genetic analysis indicated that branching was controlled by quantitative trait loci (QTL) with a normal distribution of branch numbers. Using bulked segregant analysis coupled with sequencing (BSA-seq), we identified a candidate interval of approximately 2.96 Mb on chromosome A07 linked to branching. Fine mapping narrowed this to a 172 kb region containing 29 genes, with <i>BraA07g032600.3C</i> (<i>BrTCP1</i>) as the most likely candidate. cDNA cloning of the <i>BrTCP1</i> gene revealed several variations in BCT18 compared to CX010, including a 6 bp insertion, 10 SNPs, and two single-nucleotide deletions. Expression analysis indicated that <i>BrTCP1</i> was highly expressed in the rosette stems of CX010 compared to BCT18, consistent with its role as a branching suppressor. The heterologous mutants in <i>Arabidopsis</i> confirmed the conserved role of <i>BrTCP1</i> in branch inhibition. These findings reveal that <i>BrTCP1</i> might be a key regulator of branching in flowering Chinese cabbage, providing insights into the molecular mechanisms underlying this trait and offering a framework for genetic improvement in <i>Brassica</i> crops.
ISSN:2311-7524

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