Diversity of rhizosphere microbial communities and environmental variables in wild and cultivated Scutellaria baicalensis Georgi and their effect on flavonoid

Abstract Purpose Scutellaria baicalensis Georgi in Yaozhou District in Shaanxi Province is an important Dao-di Herb. However, little is known about the differences between wild and cultivated S. baicalensis rhizosphere microbial communities, and how the differences and environmental variables affect...

Full description

Saved in:
Bibliographic Details
Main Authors: Puyang Feng, Tao Qin, Weifeng Chen, Hong Yan, Yuxin Lu, Jianqiang Liang, Yani Bai
Format: Article
Language:English
Published: BMC 2025-08-01
Series:Annals of Microbiology
Subjects:
Online Access:https://doi.org/10.1186/s13213-025-01811-z
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849238216586035200
author Puyang Feng
Tao Qin
Weifeng Chen
Hong Yan
Yuxin Lu
Jianqiang Liang
Yani Bai
author_facet Puyang Feng
Tao Qin
Weifeng Chen
Hong Yan
Yuxin Lu
Jianqiang Liang
Yani Bai
author_sort Puyang Feng
collection DOAJ
description Abstract Purpose Scutellaria baicalensis Georgi in Yaozhou District in Shaanxi Province is an important Dao-di Herb. However, little is known about the differences between wild and cultivated S. baicalensis rhizosphere microbial communities, and how the differences and environmental variables affect the accumulation of flavonoid active ingredients. Methods We analyzed the interactions between rhizosphere microbial communities- environmental variables, environmental variables- flavonoid active ingredients of wild and cultivated S. baicalensis by high performance liquid chromatography, high-throughput sequencing and multianalytical approach. Results Wild S. baicalensis contained a significantly higher content of scutellarin and baicalin compared to cultivated. Bacterial genera Sphingomonas, RB41 and fungal genera Mortierella, Cordyceps, Phialophora were higher in wild habitat than those of cultivated. The diversity of wild S. baicalensis rhizosphere fungal communities was higher, while the diversity and richness of cultivated rhizosphere bacterial communities were higher. The difference of rhizosphere microbial communities in wild and cultivated habitats may lead to differences in soil nitrogen, phosphorus and potassium nutrients. The KEGG pathways of rhizosphere bacterial communities were mainly enriched in global and overview maps. The relative abundance and species of functional species in the saprotroph in wild S. baicalensis were higher than those in cultivated. Soil SOM, TP, TK and altitude were significantly positively correlated with flavonoid contents (P < 0.05). Conclusions Increasing the application of organic matter, TN: TP and TN: TK ratio was suggested in the future cultivation, meanwhile pH value of soil should be adjusted. Besides, it is speculated that Sphingomona has potential application value in the cultivated S. baicalensis.
format Article
id doaj-art-45c4e20c9b8848fb9cad4ac829131670
institution Kabale University
issn 1869-2044
language English
publishDate 2025-08-01
publisher BMC
record_format Article
series Annals of Microbiology
spelling doaj-art-45c4e20c9b8848fb9cad4ac8291316702025-08-20T04:01:42ZengBMCAnnals of Microbiology1869-20442025-08-0175111310.1186/s13213-025-01811-zDiversity of rhizosphere microbial communities and environmental variables in wild and cultivated Scutellaria baicalensis Georgi and their effect on flavonoidPuyang Feng0Tao Qin1Weifeng Chen2Hong Yan3Yuxin Lu4Jianqiang Liang5Yani Bai6Bio-Agriculture Institute of ShaanxiBio-Agriculture Institute of ShaanxiBio-Agriculture Institute of ShaanxiBio-Agriculture Institute of ShaanxiBio-Agriculture Institute of ShaanxiBio-Agriculture Institute of ShaanxiBio-Agriculture Institute of ShaanxiAbstract Purpose Scutellaria baicalensis Georgi in Yaozhou District in Shaanxi Province is an important Dao-di Herb. However, little is known about the differences between wild and cultivated S. baicalensis rhizosphere microbial communities, and how the differences and environmental variables affect the accumulation of flavonoid active ingredients. Methods We analyzed the interactions between rhizosphere microbial communities- environmental variables, environmental variables- flavonoid active ingredients of wild and cultivated S. baicalensis by high performance liquid chromatography, high-throughput sequencing and multianalytical approach. Results Wild S. baicalensis contained a significantly higher content of scutellarin and baicalin compared to cultivated. Bacterial genera Sphingomonas, RB41 and fungal genera Mortierella, Cordyceps, Phialophora were higher in wild habitat than those of cultivated. The diversity of wild S. baicalensis rhizosphere fungal communities was higher, while the diversity and richness of cultivated rhizosphere bacterial communities were higher. The difference of rhizosphere microbial communities in wild and cultivated habitats may lead to differences in soil nitrogen, phosphorus and potassium nutrients. The KEGG pathways of rhizosphere bacterial communities were mainly enriched in global and overview maps. The relative abundance and species of functional species in the saprotroph in wild S. baicalensis were higher than those in cultivated. Soil SOM, TP, TK and altitude were significantly positively correlated with flavonoid contents (P < 0.05). Conclusions Increasing the application of organic matter, TN: TP and TN: TK ratio was suggested in the future cultivation, meanwhile pH value of soil should be adjusted. Besides, it is speculated that Sphingomona has potential application value in the cultivated S. baicalensis.https://doi.org/10.1186/s13213-025-01811-zRhizosphere microbial communitiesMedicinal plantsEnvironmental variablesAccumulation in flavonoid active ingredients
spellingShingle Puyang Feng
Tao Qin
Weifeng Chen
Hong Yan
Yuxin Lu
Jianqiang Liang
Yani Bai
Diversity of rhizosphere microbial communities and environmental variables in wild and cultivated Scutellaria baicalensis Georgi and their effect on flavonoid
Annals of Microbiology
Rhizosphere microbial communities
Medicinal plants
Environmental variables
Accumulation in flavonoid active ingredients
title Diversity of rhizosphere microbial communities and environmental variables in wild and cultivated Scutellaria baicalensis Georgi and their effect on flavonoid
title_full Diversity of rhizosphere microbial communities and environmental variables in wild and cultivated Scutellaria baicalensis Georgi and their effect on flavonoid
title_fullStr Diversity of rhizosphere microbial communities and environmental variables in wild and cultivated Scutellaria baicalensis Georgi and their effect on flavonoid
title_full_unstemmed Diversity of rhizosphere microbial communities and environmental variables in wild and cultivated Scutellaria baicalensis Georgi and their effect on flavonoid
title_short Diversity of rhizosphere microbial communities and environmental variables in wild and cultivated Scutellaria baicalensis Georgi and their effect on flavonoid
title_sort diversity of rhizosphere microbial communities and environmental variables in wild and cultivated scutellaria baicalensis georgi and their effect on flavonoid
topic Rhizosphere microbial communities
Medicinal plants
Environmental variables
Accumulation in flavonoid active ingredients
url https://doi.org/10.1186/s13213-025-01811-z
work_keys_str_mv AT puyangfeng diversityofrhizospheremicrobialcommunitiesandenvironmentalvariablesinwildandcultivatedscutellariabaicalensisgeorgiandtheireffectonflavonoid
AT taoqin diversityofrhizospheremicrobialcommunitiesandenvironmentalvariablesinwildandcultivatedscutellariabaicalensisgeorgiandtheireffectonflavonoid
AT weifengchen diversityofrhizospheremicrobialcommunitiesandenvironmentalvariablesinwildandcultivatedscutellariabaicalensisgeorgiandtheireffectonflavonoid
AT hongyan diversityofrhizospheremicrobialcommunitiesandenvironmentalvariablesinwildandcultivatedscutellariabaicalensisgeorgiandtheireffectonflavonoid
AT yuxinlu diversityofrhizospheremicrobialcommunitiesandenvironmentalvariablesinwildandcultivatedscutellariabaicalensisgeorgiandtheireffectonflavonoid
AT jianqiangliang diversityofrhizospheremicrobialcommunitiesandenvironmentalvariablesinwildandcultivatedscutellariabaicalensisgeorgiandtheireffectonflavonoid
AT yanibai diversityofrhizospheremicrobialcommunitiesandenvironmentalvariablesinwildandcultivatedscutellariabaicalensisgeorgiandtheireffectonflavonoid