Development of a novel sugar-free functional beverage containing pistachio green hull extract

This study aimed to develop a novel phenolic-rich functional beverage utilizing pistachio green hull extract (PGHE) and to evaluate its physicochemical and sensory properties. An acute oral toxicity evaluation demonstrated that LD50 of extract is greater than 5000 mg/kg body weight of Wistar rats. T...

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Main Authors: Maedeh Tavakoli, Mohsen Barzegar, Mohammad Ali Sahari, Narges Hosseinmardi
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Applied Food Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772502225002343
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author Maedeh Tavakoli
Mohsen Barzegar
Mohammad Ali Sahari
Narges Hosseinmardi
author_facet Maedeh Tavakoli
Mohsen Barzegar
Mohammad Ali Sahari
Narges Hosseinmardi
author_sort Maedeh Tavakoli
collection DOAJ
description This study aimed to develop a novel phenolic-rich functional beverage utilizing pistachio green hull extract (PGHE) and to evaluate its physicochemical and sensory properties. An acute oral toxicity evaluation demonstrated that LD50 of extract is greater than 5000 mg/kg body weight of Wistar rats. The optimized formulation of the functional beverage was determined using response surface methodology (RSM). Different concentrations of PGHE, stevia, mannitol, and vanilla, along with constant concentrations of Arabic gum (0.5 % w/v) and ascorbic acid (0.04 % w/v), were used to prepare pistachio green hull based beverages. Total phenolic content (TPC) and IC50 were considered as dependent variables. The optimized formulation, based on maximum TPC and antioxidant activity, was identified, and sensory results indicated that functional beverage 1 (FB1, containing 8.0 % PGHE, 0.18 % stevia, 2.0 % mannitol, and 0.39 % w/v vanilla) is the best formulation. The FB1 showed L*= 14.83 ± 0.19, a*= 1.53 ± 0.05, b*= 12.45 ± 0.10, pH= 4.63 ± 0.01, and °Brix= 11.95 ± 0.07. To enhance the marketability of the optimized functional beverage, the concentration of PGHE and the flavor type were changed. Sensory evaluation of prepared formulations showed that functional beverage A (FB-A, containing 2.0 % w/v PGHE and 2.0 %v/v saffron extract) is more acceptable. The stability assessment of the optimized formulation, FB1, during two months of storage (4 °C) showed that TPC and IC50 remained unchanged. Overall, FB1 formulation has the potential to serve as a beneficial nutraceutical beverage, harnessing the health-promoting effects of all its ingredients, particularly PGHE.
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spelling doaj-art-45b6288a276d41ff878ca0add89b9c592025-08-20T03:20:16ZengElsevierApplied Food Research2772-50222025-06-015110092610.1016/j.afres.2025.100926Development of a novel sugar-free functional beverage containing pistachio green hull extractMaedeh Tavakoli0Mohsen Barzegar1Mohammad Ali Sahari2Narges Hosseinmardi3Department of Food Science and Technology, Tarbiat Modares University, Tehran, IranDepartment of Food Science and Technology, Tarbiat Modares University, Tehran, Iran; Corresponding author.Department of Food Science and Technology, Tarbiat Modares University, Tehran, IranDepartment of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, IranThis study aimed to develop a novel phenolic-rich functional beverage utilizing pistachio green hull extract (PGHE) and to evaluate its physicochemical and sensory properties. An acute oral toxicity evaluation demonstrated that LD50 of extract is greater than 5000 mg/kg body weight of Wistar rats. The optimized formulation of the functional beverage was determined using response surface methodology (RSM). Different concentrations of PGHE, stevia, mannitol, and vanilla, along with constant concentrations of Arabic gum (0.5 % w/v) and ascorbic acid (0.04 % w/v), were used to prepare pistachio green hull based beverages. Total phenolic content (TPC) and IC50 were considered as dependent variables. The optimized formulation, based on maximum TPC and antioxidant activity, was identified, and sensory results indicated that functional beverage 1 (FB1, containing 8.0 % PGHE, 0.18 % stevia, 2.0 % mannitol, and 0.39 % w/v vanilla) is the best formulation. The FB1 showed L*= 14.83 ± 0.19, a*= 1.53 ± 0.05, b*= 12.45 ± 0.10, pH= 4.63 ± 0.01, and °Brix= 11.95 ± 0.07. To enhance the marketability of the optimized functional beverage, the concentration of PGHE and the flavor type were changed. Sensory evaluation of prepared formulations showed that functional beverage A (FB-A, containing 2.0 % w/v PGHE and 2.0 %v/v saffron extract) is more acceptable. The stability assessment of the optimized formulation, FB1, during two months of storage (4 °C) showed that TPC and IC50 remained unchanged. Overall, FB1 formulation has the potential to serve as a beneficial nutraceutical beverage, harnessing the health-promoting effects of all its ingredients, particularly PGHE.http://www.sciencedirect.com/science/article/pii/S2772502225002343AntioxidantPhenolic compoundsPlant-based beverageRSMSteviaSensory evaluation
spellingShingle Maedeh Tavakoli
Mohsen Barzegar
Mohammad Ali Sahari
Narges Hosseinmardi
Development of a novel sugar-free functional beverage containing pistachio green hull extract
Applied Food Research
Antioxidant
Phenolic compounds
Plant-based beverage
RSM
Stevia
Sensory evaluation
title Development of a novel sugar-free functional beverage containing pistachio green hull extract
title_full Development of a novel sugar-free functional beverage containing pistachio green hull extract
title_fullStr Development of a novel sugar-free functional beverage containing pistachio green hull extract
title_full_unstemmed Development of a novel sugar-free functional beverage containing pistachio green hull extract
title_short Development of a novel sugar-free functional beverage containing pistachio green hull extract
title_sort development of a novel sugar free functional beverage containing pistachio green hull extract
topic Antioxidant
Phenolic compounds
Plant-based beverage
RSM
Stevia
Sensory evaluation
url http://www.sciencedirect.com/science/article/pii/S2772502225002343
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