Sequential testing with Xpert MTB/RIF assay for diagnosis of tuberculous meningitis in Maharaj Nakorn Chiang Mai University Hospital

Abstract Early diagnosis and appropriate treatment are essential for reducing morbidity and mortality in tuberculous meningitis (TBM). This study aimed to evaluate the diagnostic performance of the Xpert MTB/RIF assay for the diagnosis of TBM in patients with subacute lymphocytic meningitis. This cr...

Full description

Saved in:
Bibliographic Details
Main Authors: Poramed Winichakoon, Kanokwan Watcharasaksilp, Sunisa Butphet, Kanlaya Wongworapat, Chansom Pantip, Phadungkiat Khamnoi, Khuanchai Supparatpinyo, Parichat Salee
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-025-87739-5
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832571648588382208
author Poramed Winichakoon
Kanokwan Watcharasaksilp
Sunisa Butphet
Kanlaya Wongworapat
Chansom Pantip
Phadungkiat Khamnoi
Khuanchai Supparatpinyo
Parichat Salee
author_facet Poramed Winichakoon
Kanokwan Watcharasaksilp
Sunisa Butphet
Kanlaya Wongworapat
Chansom Pantip
Phadungkiat Khamnoi
Khuanchai Supparatpinyo
Parichat Salee
author_sort Poramed Winichakoon
collection DOAJ
description Abstract Early diagnosis and appropriate treatment are essential for reducing morbidity and mortality in tuberculous meningitis (TBM). This study aimed to evaluate the diagnostic performance of the Xpert MTB/RIF assay for the diagnosis of TBM in patients with subacute lymphocytic meningitis. This cross-sectional study included 65 cerebrospinal fluid (CSF) specimens from patients at Maharaj Nakorn Chiang Mai University Hospital, Thailand, between January 2015 and March 2016. Mycobacteria growth indicator tube (MGIT) culture was used as the reference standard. Sensitivity, specificity, and agreement between Xpert MTB/RIF and MGIT culture were calculated. Sequential testing using a TBM score, followed by Xpert MTB/RIF was also analyzed. Xpert MTB/RIF demonstrated 83.33% sensitivity (95% CI 57.19–98.22) and 96.23% specificity (95% CI 87.02–99.54). Agreement between Xpert MTB/RIF and MGIT culture was 93.85% (p < 0.001), with a kappa score of 0.80 (95% CI 0.60–0.99). Sequential testing with a TBM score cut-off of 6, followed by Xpert MTB/RIF improved specificity from 96.23 to 97.15%. The Xpert MTB/RIF assay is a rapid and valuable tool for detecting Mycobacterium tuberculosis in centrifuged CSF specimens. A diagnostic algorithm incorporating the TBM score enhances performance, balancing sensitivity and specificity, and could improve patient outcomes in resource-limited settings.
format Article
id doaj-art-45b152e8d4ee4c468e0a45ee47b4a52a
institution Kabale University
issn 2045-2322
language English
publishDate 2025-01-01
publisher Nature Portfolio
record_format Article
series Scientific Reports
spelling doaj-art-45b152e8d4ee4c468e0a45ee47b4a52a2025-02-02T12:24:03ZengNature PortfolioScientific Reports2045-23222025-01-011511910.1038/s41598-025-87739-5Sequential testing with Xpert MTB/RIF assay for diagnosis of tuberculous meningitis in Maharaj Nakorn Chiang Mai University HospitalPoramed Winichakoon0Kanokwan Watcharasaksilp1Sunisa Butphet2Kanlaya Wongworapat3Chansom Pantip4Phadungkiat Khamnoi5Khuanchai Supparatpinyo6Parichat Salee7Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Faculty of Medicine, Chiang Mai UniversityDivision of Neurology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai UniversityResearch Institute for Health Sciences, Chiang Mai UniversityResearch Institute for Health Sciences, Chiang Mai UniversityResearch Institute for Health Sciences, Chiang Mai UniversityMicrobiology Section, Diagnostic Laboratory, Maharaj Nakorn Chiang Mai University HospitalDivision of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Faculty of Medicine, Chiang Mai UniversityDivision of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Faculty of Medicine, Chiang Mai UniversityAbstract Early diagnosis and appropriate treatment are essential for reducing morbidity and mortality in tuberculous meningitis (TBM). This study aimed to evaluate the diagnostic performance of the Xpert MTB/RIF assay for the diagnosis of TBM in patients with subacute lymphocytic meningitis. This cross-sectional study included 65 cerebrospinal fluid (CSF) specimens from patients at Maharaj Nakorn Chiang Mai University Hospital, Thailand, between January 2015 and March 2016. Mycobacteria growth indicator tube (MGIT) culture was used as the reference standard. Sensitivity, specificity, and agreement between Xpert MTB/RIF and MGIT culture were calculated. Sequential testing using a TBM score, followed by Xpert MTB/RIF was also analyzed. Xpert MTB/RIF demonstrated 83.33% sensitivity (95% CI 57.19–98.22) and 96.23% specificity (95% CI 87.02–99.54). Agreement between Xpert MTB/RIF and MGIT culture was 93.85% (p < 0.001), with a kappa score of 0.80 (95% CI 0.60–0.99). Sequential testing with a TBM score cut-off of 6, followed by Xpert MTB/RIF improved specificity from 96.23 to 97.15%. The Xpert MTB/RIF assay is a rapid and valuable tool for detecting Mycobacterium tuberculosis in centrifuged CSF specimens. A diagnostic algorithm incorporating the TBM score enhances performance, balancing sensitivity and specificity, and could improve patient outcomes in resource-limited settings.https://doi.org/10.1038/s41598-025-87739-5MeningitisTuberculosisXpert MTB/RIF
spellingShingle Poramed Winichakoon
Kanokwan Watcharasaksilp
Sunisa Butphet
Kanlaya Wongworapat
Chansom Pantip
Phadungkiat Khamnoi
Khuanchai Supparatpinyo
Parichat Salee
Sequential testing with Xpert MTB/RIF assay for diagnosis of tuberculous meningitis in Maharaj Nakorn Chiang Mai University Hospital
Scientific Reports
Meningitis
Tuberculosis
Xpert MTB/RIF
title Sequential testing with Xpert MTB/RIF assay for diagnosis of tuberculous meningitis in Maharaj Nakorn Chiang Mai University Hospital
title_full Sequential testing with Xpert MTB/RIF assay for diagnosis of tuberculous meningitis in Maharaj Nakorn Chiang Mai University Hospital
title_fullStr Sequential testing with Xpert MTB/RIF assay for diagnosis of tuberculous meningitis in Maharaj Nakorn Chiang Mai University Hospital
title_full_unstemmed Sequential testing with Xpert MTB/RIF assay for diagnosis of tuberculous meningitis in Maharaj Nakorn Chiang Mai University Hospital
title_short Sequential testing with Xpert MTB/RIF assay for diagnosis of tuberculous meningitis in Maharaj Nakorn Chiang Mai University Hospital
title_sort sequential testing with xpert mtb rif assay for diagnosis of tuberculous meningitis in maharaj nakorn chiang mai university hospital
topic Meningitis
Tuberculosis
Xpert MTB/RIF
url https://doi.org/10.1038/s41598-025-87739-5
work_keys_str_mv AT poramedwinichakoon sequentialtestingwithxpertmtbrifassayfordiagnosisoftuberculousmeningitisinmaharajnakornchiangmaiuniversityhospital
AT kanokwanwatcharasaksilp sequentialtestingwithxpertmtbrifassayfordiagnosisoftuberculousmeningitisinmaharajnakornchiangmaiuniversityhospital
AT sunisabutphet sequentialtestingwithxpertmtbrifassayfordiagnosisoftuberculousmeningitisinmaharajnakornchiangmaiuniversityhospital
AT kanlayawongworapat sequentialtestingwithxpertmtbrifassayfordiagnosisoftuberculousmeningitisinmaharajnakornchiangmaiuniversityhospital
AT chansompantip sequentialtestingwithxpertmtbrifassayfordiagnosisoftuberculousmeningitisinmaharajnakornchiangmaiuniversityhospital
AT phadungkiatkhamnoi sequentialtestingwithxpertmtbrifassayfordiagnosisoftuberculousmeningitisinmaharajnakornchiangmaiuniversityhospital
AT khuanchaisupparatpinyo sequentialtestingwithxpertmtbrifassayfordiagnosisoftuberculousmeningitisinmaharajnakornchiangmaiuniversityhospital
AT parichatsalee sequentialtestingwithxpertmtbrifassayfordiagnosisoftuberculousmeningitisinmaharajnakornchiangmaiuniversityhospital