MicroRNA-497-5p Is Downregulated in Hepatocellular Carcinoma and Associated with Tumorigenesis and Poor Prognosis in Patients
Background. MicroRNAs (miRNAs) have been demonstrated to exhibit important regulatory roles in multiple malignancies, including hepatocellular carcinoma (HCC). hsa-miR-497-5p was reported to involve in cancer progression and poor prognosis in many kinds of tumors. However, the expression and its cli...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2021/6670390 |
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| author | Lin-Lin Tian Bin Qian Xiao-Hui Jiang Yu-Shan Liu Tong Chen Cheng-You Jia Ya-Li Zhou Ji-Bin Liu Yu-Shui Ma Da Fu Sen-Tai Ding |
| author_facet | Lin-Lin Tian Bin Qian Xiao-Hui Jiang Yu-Shan Liu Tong Chen Cheng-You Jia Ya-Li Zhou Ji-Bin Liu Yu-Shui Ma Da Fu Sen-Tai Ding |
| author_sort | Lin-Lin Tian |
| collection | DOAJ |
| description | Background. MicroRNAs (miRNAs) have been demonstrated to exhibit important regulatory roles in multiple malignancies, including hepatocellular carcinoma (HCC). hsa-miR-497-5p was reported to involve in cancer progression and poor prognosis in many kinds of tumors. However, the expression and its clinical significance of hsa-miR-497-5p in HCC remain unclear. Methods. In the present study, we investigated the expression of hsa-miR-497-5p in HCC and analyzed the correction of clinical features with prognosis. The expression levels of hsa-miR-497-5p and potential target genes were analyzed in HCC and adjacent noncancerous tissues using The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) datasets. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to analyze hsa-miR-497-5p levels in 328 HCC tissues and 30 paired adjacent noncancer tissues. Overall survival (OS) and progression-free survival (PFS) of patients with HCC were assessed using the Kaplan-Meier method and the log-rank test. Results. The hsa-miR-497-5p expression levels were decreased, and its target genes ACTG1, CSNK1D, PPP1CC, and BIRC5 were upregulated in HCC tissues compared with normal tissues. Lower levels of hsa-miR-497-5p expression and higher levels of the four target genes were significantly associated with higher tumor diameter. Moreover, patients with lower hsa-miR-497-5p expression and higher target genes levels had shorter OS. Conclusion. The expression levels of hsa-miR-497-5p may play an important regulatory role in HCC and are closely correlated with HCC progression and poor prognosis in patients. The hsa-miR-497-5p may be a specific therapeutic target for the treatment of HCC. |
| format | Article |
| id | doaj-art-459aa2d1992f44ebbb9e539b1e3b57c9 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-459aa2d1992f44ebbb9e539b1e3b57c92025-08-20T03:36:11ZengWileyInternational Journal of Genomics2314-436X2314-43782021-01-01202110.1155/2021/66703906670390MicroRNA-497-5p Is Downregulated in Hepatocellular Carcinoma and Associated with Tumorigenesis and Poor Prognosis in PatientsLin-Lin Tian0Bin Qian1Xiao-Hui Jiang2Yu-Shan Liu3Tong Chen4Cheng-You Jia5Ya-Li Zhou6Ji-Bin Liu7Yu-Shui Ma8Da Fu9Sen-Tai Ding10Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, ChinaDepartment of General Surgery, Shanghai Eighth People’s Hospital, Shanghai 200235, ChinaDepartment of Gastrointestinal Surgery, Affiliated Tumor Hospital of Nantong University, Nantong 226631, ChinaDepartment of Pathology, Nantong Tumor Hospital, Nantong 226631, ChinaDepartment of Pediatric Surgery, Shanghai Children’s Hospital, Shanghai Jiao Tong University, ChinaCentral Laboratory for Medical Research, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, ChinaDepartment of Microbiology, Faculty of Basic Medical Sciences, Guilin Medical University, Guilin 541001, ChinaDepartment of Gastrointestinal Surgery, Affiliated Tumor Hospital of Nantong University, Nantong 226631, ChinaCentral Laboratory for Medical Research, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, ChinaCentral Laboratory for Medical Research, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, ChinaDepartment of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, ChinaBackground. MicroRNAs (miRNAs) have been demonstrated to exhibit important regulatory roles in multiple malignancies, including hepatocellular carcinoma (HCC). hsa-miR-497-5p was reported to involve in cancer progression and poor prognosis in many kinds of tumors. However, the expression and its clinical significance of hsa-miR-497-5p in HCC remain unclear. Methods. In the present study, we investigated the expression of hsa-miR-497-5p in HCC and analyzed the correction of clinical features with prognosis. The expression levels of hsa-miR-497-5p and potential target genes were analyzed in HCC and adjacent noncancerous tissues using The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) datasets. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to analyze hsa-miR-497-5p levels in 328 HCC tissues and 30 paired adjacent noncancer tissues. Overall survival (OS) and progression-free survival (PFS) of patients with HCC were assessed using the Kaplan-Meier method and the log-rank test. Results. The hsa-miR-497-5p expression levels were decreased, and its target genes ACTG1, CSNK1D, PPP1CC, and BIRC5 were upregulated in HCC tissues compared with normal tissues. Lower levels of hsa-miR-497-5p expression and higher levels of the four target genes were significantly associated with higher tumor diameter. Moreover, patients with lower hsa-miR-497-5p expression and higher target genes levels had shorter OS. Conclusion. The expression levels of hsa-miR-497-5p may play an important regulatory role in HCC and are closely correlated with HCC progression and poor prognosis in patients. The hsa-miR-497-5p may be a specific therapeutic target for the treatment of HCC.http://dx.doi.org/10.1155/2021/6670390 |
| spellingShingle | Lin-Lin Tian Bin Qian Xiao-Hui Jiang Yu-Shan Liu Tong Chen Cheng-You Jia Ya-Li Zhou Ji-Bin Liu Yu-Shui Ma Da Fu Sen-Tai Ding MicroRNA-497-5p Is Downregulated in Hepatocellular Carcinoma and Associated with Tumorigenesis and Poor Prognosis in Patients International Journal of Genomics |
| title | MicroRNA-497-5p Is Downregulated in Hepatocellular Carcinoma and Associated with Tumorigenesis and Poor Prognosis in Patients |
| title_full | MicroRNA-497-5p Is Downregulated in Hepatocellular Carcinoma and Associated with Tumorigenesis and Poor Prognosis in Patients |
| title_fullStr | MicroRNA-497-5p Is Downregulated in Hepatocellular Carcinoma and Associated with Tumorigenesis and Poor Prognosis in Patients |
| title_full_unstemmed | MicroRNA-497-5p Is Downregulated in Hepatocellular Carcinoma and Associated with Tumorigenesis and Poor Prognosis in Patients |
| title_short | MicroRNA-497-5p Is Downregulated in Hepatocellular Carcinoma and Associated with Tumorigenesis and Poor Prognosis in Patients |
| title_sort | microrna 497 5p is downregulated in hepatocellular carcinoma and associated with tumorigenesis and poor prognosis in patients |
| url | http://dx.doi.org/10.1155/2021/6670390 |
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