The immunologic characteristic of the stages of chronic hepatitis C and an assessment of immune system factors as prognostic criteria of a current disease
In the present study factors of immune system in peripheral blood (PB) of patients with chronic hepatitis C (CHС) with the different degree of fibrosis were determined. Patients with CHC have been divided into groups, according to the stages of disease: weak fibrosis F1 (n=32), moderate fibrosis F2...
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Journal Infectology
2014-09-01
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| Series: | Журнал инфектологии |
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| Online Access: | https://journal.niidi.ru/jofin/article/view/326 |
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| author | V. Yu. Nikitin I. A. Sukhina V. N. Tsigan D. A. Gusev |
| author_facet | V. Yu. Nikitin I. A. Sukhina V. N. Tsigan D. A. Gusev |
| author_sort | V. Yu. Nikitin |
| collection | DOAJ |
| description | In the present study factors of immune system in peripheral blood (PB) of patients with chronic hepatitis C (CHС) with the different degree of fibrosis were determined. Patients with CHC have been divided into groups, according to the stages of disease: weak fibrosis F1 (n=32), moderate fibrosis F2 (n=22), heavy fibrosis F3 (n=19) and cirrhosis of liver F4 (n=13). The following subpopulations of T-lymphocytes have been investigated: CD3+CD4+, CD3+CD8+, CD3+CD16+56+, CD3+CD25+, CD3+HLA-DR+, CD3+CD4+CD25+, CD3+CD8+CD25+, CD3+CD4+HLA-DR+, CD3+CD8+HLA-DR+, CD3+CD95+. Besides, the subpopulations of В-cells (CD19+, CD3CD25+, CD19+CD95+), Ig A, IgM, IgG, highly-, medial-, low-molecular circulating immune complexes (CIC), cytokines Th1 (IFN-γ),Th2 (IL-4, IL-10) types, as well as proinflammatory cytokines TNF-α, IL-1β were defined. This research showed that the patient with the high degree of fibrosis have the increased quantity NKT (CD3+CD16+56+) and total count of activated T-cells HLA-DR (CD3+HLA-DR+) and the tendency for growing percentages of activated cytotoxic T-cells (CD8+CD25+ and CD8+HLA-DR+), which reflects the evolution of autoimmune reactions as the disease continues.These results have shown that in the higher stages of fibrosis the patients with CHC increased activity of T-cells immunity and constant antigen-specific or non-specific stimulation of T-lymphocytes may play a significant role in pathogenesis of CHC.With the increase of the fibrosis degree in patient with CHС, levels of IgA, IgG, medium-, low-molecular CIC, cytokines IFN-γ, IL-10 were increasing and the quantity of activated B-cells (CD3–CD25+) were decreasing. The research shows the domination of the B-cells response while the function of B-lymphocytes are injured and also are observed Th1/Th2 disbalance at all stages of disease. Besides, the direct correlation between CD95-antigen (Fas/APO-1) and activated CD3+CD4+CD25+, CD3+CD8+CD25+, CD3CD25+ subsets of lymphocytes was found. The established changes of the factors of the immune system can be used as the prognostic criteria of CHC. |
| format | Article |
| id | doaj-art-458be080211e4cf2bd7c3a9f4186aacc |
| institution | DOAJ |
| issn | 2072-6732 |
| language | Russian |
| publishDate | 2014-09-01 |
| publisher | Journal Infectology |
| record_format | Article |
| series | Журнал инфектологии |
| spelling | doaj-art-458be080211e4cf2bd7c3a9f4186aacc2025-08-20T02:55:13ZrusJournal InfectologyЖурнал инфектологии2072-67322014-09-0111304010.22625/2072-6732-2009-1-1-30-40340The immunologic characteristic of the stages of chronic hepatitis C and an assessment of immune system factors as prognostic criteria of a current diseaseV. Yu. Nikitin0I. A. Sukhina1V. N. Tsigan2D. A. Gusev3Military Medical Academy by S.M. KirovMilitary Medical Academy by S.M. KirovMilitary Medical Academy by S.M. KirovMilitary Medical Academy by S.M. KirovIn the present study factors of immune system in peripheral blood (PB) of patients with chronic hepatitis C (CHС) with the different degree of fibrosis were determined. Patients with CHC have been divided into groups, according to the stages of disease: weak fibrosis F1 (n=32), moderate fibrosis F2 (n=22), heavy fibrosis F3 (n=19) and cirrhosis of liver F4 (n=13). The following subpopulations of T-lymphocytes have been investigated: CD3+CD4+, CD3+CD8+, CD3+CD16+56+, CD3+CD25+, CD3+HLA-DR+, CD3+CD4+CD25+, CD3+CD8+CD25+, CD3+CD4+HLA-DR+, CD3+CD8+HLA-DR+, CD3+CD95+. Besides, the subpopulations of В-cells (CD19+, CD3CD25+, CD19+CD95+), Ig A, IgM, IgG, highly-, medial-, low-molecular circulating immune complexes (CIC), cytokines Th1 (IFN-γ),Th2 (IL-4, IL-10) types, as well as proinflammatory cytokines TNF-α, IL-1β were defined. This research showed that the patient with the high degree of fibrosis have the increased quantity NKT (CD3+CD16+56+) and total count of activated T-cells HLA-DR (CD3+HLA-DR+) and the tendency for growing percentages of activated cytotoxic T-cells (CD8+CD25+ and CD8+HLA-DR+), which reflects the evolution of autoimmune reactions as the disease continues.These results have shown that in the higher stages of fibrosis the patients with CHC increased activity of T-cells immunity and constant antigen-specific or non-specific stimulation of T-lymphocytes may play a significant role in pathogenesis of CHC.With the increase of the fibrosis degree in patient with CHС, levels of IgA, IgG, medium-, low-molecular CIC, cytokines IFN-γ, IL-10 were increasing and the quantity of activated B-cells (CD3–CD25+) were decreasing. The research shows the domination of the B-cells response while the function of B-lymphocytes are injured and also are observed Th1/Th2 disbalance at all stages of disease. Besides, the direct correlation between CD95-antigen (Fas/APO-1) and activated CD3+CD4+CD25+, CD3+CD8+CD25+, CD3CD25+ subsets of lymphocytes was found. The established changes of the factors of the immune system can be used as the prognostic criteria of CHC.https://journal.niidi.ru/jofin/article/view/326factors of immune systemchronic hepatitis cfibrosiscirrhosis |
| spellingShingle | V. Yu. Nikitin I. A. Sukhina V. N. Tsigan D. A. Gusev The immunologic characteristic of the stages of chronic hepatitis C and an assessment of immune system factors as prognostic criteria of a current disease Журнал инфектологии factors of immune system chronic hepatitis c fibrosis cirrhosis |
| title | The immunologic characteristic of the stages of chronic hepatitis C and an assessment of immune system factors as prognostic criteria of a current disease |
| title_full | The immunologic characteristic of the stages of chronic hepatitis C and an assessment of immune system factors as prognostic criteria of a current disease |
| title_fullStr | The immunologic characteristic of the stages of chronic hepatitis C and an assessment of immune system factors as prognostic criteria of a current disease |
| title_full_unstemmed | The immunologic characteristic of the stages of chronic hepatitis C and an assessment of immune system factors as prognostic criteria of a current disease |
| title_short | The immunologic characteristic of the stages of chronic hepatitis C and an assessment of immune system factors as prognostic criteria of a current disease |
| title_sort | immunologic characteristic of the stages of chronic hepatitis c and an assessment of immune system factors as prognostic criteria of a current disease |
| topic | factors of immune system chronic hepatitis c fibrosis cirrhosis |
| url | https://journal.niidi.ru/jofin/article/view/326 |
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