The Protective Effect of Naringenin-Oxime on Cisplatin-Induced Toxicity in Rats
The aim of this study is to examine the protective effect of naringenin-oxime (NOX) on cisplatin-induced major organ toxicity and DNA damage in rats. Thirty-five male Wistar albino rats were equally split into five groups as follows: control (i.p., 0.1 ml of saline), Cis administration (i.p., 7 mg/k...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2017/9478958 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849407102504665088 |
|---|---|
| author | Ismail Koyuncu Abdurrahim Kocyigit Ataman Gonel Erkan Arslan Mustafa Durgun |
| author_facet | Ismail Koyuncu Abdurrahim Kocyigit Ataman Gonel Erkan Arslan Mustafa Durgun |
| author_sort | Ismail Koyuncu |
| collection | DOAJ |
| description | The aim of this study is to examine the protective effect of naringenin-oxime (NOX) on cisplatin-induced major organ toxicity and DNA damage in rats. Thirty-five male Wistar albino rats were equally split into five groups as follows: control (i.p., 0.1 ml of saline), Cis administration (i.p., 7 mg/kg b.w.), NOX treatment (i.p., 20 mg/kg b.w., daily for ten days), Cis + NOX20, and Cis + NOX40 combination (i.p., 20 and 40 mg/kg b.w., daily for ten days). Serum and peripheral blood mononuclear leukocytes (PBMC) were obtained from blood. Malondialdehyde, glutathione, total antioxidant and oxidant status, and catalase were measured in serum, liver, and kidney, and oxidative stress index was calculated. In parallel, paraoxonase and arylesterase activities were tested in liver and serum. We used 8-OHdOG as a marker for DNA damage in serum via ELISA and in PMBC via comet assay. Treatment with Cis elevated the levels of serum biochemical parameters, oxidative stress, and DNA damage. Pretreatments of NOX restored biochemical and oxidative stress parameters in serum, renal, and liver tissues (p<0.01) and reduced 8-OHdG level, a finding further supported by comet assay in PBMC. Observations of the present study support the fact that treatment with NOX prevents Cis-induced hepatotoxicity, nephrotoxicity, and genotoxicity by restoring antioxidant system. |
| format | Article |
| id | doaj-art-458b7e91ef924fc28ad51c36c558f3f1 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-458b7e91ef924fc28ad51c36c558f3f12025-08-20T03:36:11ZengWileyBiochemistry Research International2090-22472090-22552017-01-01201710.1155/2017/94789589478958The Protective Effect of Naringenin-Oxime on Cisplatin-Induced Toxicity in RatsIsmail Koyuncu0Abdurrahim Kocyigit1Ataman Gonel2Erkan Arslan3Mustafa Durgun4Department of Biochemistry, Faculty of Medicine, Harran University, Sanliurfa, TurkeyDepartment of Medical Biochemistry, Faculty of Medicine, Bezmialem Vakif University, Istanbul, TurkeyDepartment of Biochemistry, Faculty of Medicine, Harran University, Sanliurfa, TurkeyDepartment of Urology, Faculty of Medicine, Harran University, Sanliurfa, TurkeyDepartment of Chemistry, Faculty of Science and Literature, Harran University, Sanliurfa, TurkeyThe aim of this study is to examine the protective effect of naringenin-oxime (NOX) on cisplatin-induced major organ toxicity and DNA damage in rats. Thirty-five male Wistar albino rats were equally split into five groups as follows: control (i.p., 0.1 ml of saline), Cis administration (i.p., 7 mg/kg b.w.), NOX treatment (i.p., 20 mg/kg b.w., daily for ten days), Cis + NOX20, and Cis + NOX40 combination (i.p., 20 and 40 mg/kg b.w., daily for ten days). Serum and peripheral blood mononuclear leukocytes (PBMC) were obtained from blood. Malondialdehyde, glutathione, total antioxidant and oxidant status, and catalase were measured in serum, liver, and kidney, and oxidative stress index was calculated. In parallel, paraoxonase and arylesterase activities were tested in liver and serum. We used 8-OHdOG as a marker for DNA damage in serum via ELISA and in PMBC via comet assay. Treatment with Cis elevated the levels of serum biochemical parameters, oxidative stress, and DNA damage. Pretreatments of NOX restored biochemical and oxidative stress parameters in serum, renal, and liver tissues (p<0.01) and reduced 8-OHdG level, a finding further supported by comet assay in PBMC. Observations of the present study support the fact that treatment with NOX prevents Cis-induced hepatotoxicity, nephrotoxicity, and genotoxicity by restoring antioxidant system.http://dx.doi.org/10.1155/2017/9478958 |
| spellingShingle | Ismail Koyuncu Abdurrahim Kocyigit Ataman Gonel Erkan Arslan Mustafa Durgun The Protective Effect of Naringenin-Oxime on Cisplatin-Induced Toxicity in Rats Biochemistry Research International |
| title | The Protective Effect of Naringenin-Oxime on Cisplatin-Induced Toxicity in Rats |
| title_full | The Protective Effect of Naringenin-Oxime on Cisplatin-Induced Toxicity in Rats |
| title_fullStr | The Protective Effect of Naringenin-Oxime on Cisplatin-Induced Toxicity in Rats |
| title_full_unstemmed | The Protective Effect of Naringenin-Oxime on Cisplatin-Induced Toxicity in Rats |
| title_short | The Protective Effect of Naringenin-Oxime on Cisplatin-Induced Toxicity in Rats |
| title_sort | protective effect of naringenin oxime on cisplatin induced toxicity in rats |
| url | http://dx.doi.org/10.1155/2017/9478958 |
| work_keys_str_mv | AT ismailkoyuncu theprotectiveeffectofnaringeninoximeoncisplatininducedtoxicityinrats AT abdurrahimkocyigit theprotectiveeffectofnaringeninoximeoncisplatininducedtoxicityinrats AT atamangonel theprotectiveeffectofnaringeninoximeoncisplatininducedtoxicityinrats AT erkanarslan theprotectiveeffectofnaringeninoximeoncisplatininducedtoxicityinrats AT mustafadurgun theprotectiveeffectofnaringeninoximeoncisplatininducedtoxicityinrats AT ismailkoyuncu protectiveeffectofnaringeninoximeoncisplatininducedtoxicityinrats AT abdurrahimkocyigit protectiveeffectofnaringeninoximeoncisplatininducedtoxicityinrats AT atamangonel protectiveeffectofnaringeninoximeoncisplatininducedtoxicityinrats AT erkanarslan protectiveeffectofnaringeninoximeoncisplatininducedtoxicityinrats AT mustafadurgun protectiveeffectofnaringeninoximeoncisplatininducedtoxicityinrats |