Surface Relief Gratings of Slide-Ring Hydrogels for Label-Free Biosensing
The creation of surface relief gratings using hydrogels for label-free biomolecule detection represents a significant advance in the development of versatile, cutting-edge biosensors. Central to this innovation is the formulation of materials with enhanced mechanical properties, especially for appli...
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| Format: | Article |
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MDPI AG
2025-05-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/11/6/415 |
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| author | Aitor Cubells-Gómez María Isabel Lucío María-José Bañuls Ángel Maquieira |
| author_facet | Aitor Cubells-Gómez María Isabel Lucío María-José Bañuls Ángel Maquieira |
| author_sort | Aitor Cubells-Gómez |
| collection | DOAJ |
| description | The creation of surface relief gratings using hydrogels for label-free biomolecule detection represents a significant advance in the development of versatile, cutting-edge biosensors. Central to this innovation is the formulation of materials with enhanced mechanical properties, especially for applications in soft, wearable technologies. In this work, we have developed novel biofunctional hydrogels that incorporate slide-ring supramolecular structures into their network, enabling the production of surface relief gratings with superior mechanical characteristics for biomolecule detection without the need for labels. These hydrogels, functionalized with bovine serum albumin and goat anti-rabbit antibodies, demonstrated excellent selectivity and sensitivity toward anti-bovine serum albumin and rabbit IgGs in blood serum, evaluated using a label-free format. Remarkably, the new materials matched the analytical performance of conventional hydrogels based on static networks while offering dramatically improved toughness and elasticity, with a compressive modulus comparable to human skin. This demonstrates the potential of slide-ring hydrogels for fabricating robust, label-free biosensing platforms. Furthermore, the flexibility of this system allows for the incorporation of various recognition elements tailored to specific applications. |
| format | Article |
| id | doaj-art-4583d6f0d6894dac8caf76c67da3efbb |
| institution | OA Journals |
| issn | 2310-2861 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj-art-4583d6f0d6894dac8caf76c67da3efbb2025-08-20T02:21:11ZengMDPI AGGels2310-28612025-05-0111641510.3390/gels11060415Surface Relief Gratings of Slide-Ring Hydrogels for Label-Free BiosensingAitor Cubells-Gómez0María Isabel Lucío1María-José Bañuls2Ángel Maquieira3Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022 Valencia, SpainInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022 Valencia, SpainInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022 Valencia, SpainInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022 Valencia, SpainThe creation of surface relief gratings using hydrogels for label-free biomolecule detection represents a significant advance in the development of versatile, cutting-edge biosensors. Central to this innovation is the formulation of materials with enhanced mechanical properties, especially for applications in soft, wearable technologies. In this work, we have developed novel biofunctional hydrogels that incorporate slide-ring supramolecular structures into their network, enabling the production of surface relief gratings with superior mechanical characteristics for biomolecule detection without the need for labels. These hydrogels, functionalized with bovine serum albumin and goat anti-rabbit antibodies, demonstrated excellent selectivity and sensitivity toward anti-bovine serum albumin and rabbit IgGs in blood serum, evaluated using a label-free format. Remarkably, the new materials matched the analytical performance of conventional hydrogels based on static networks while offering dramatically improved toughness and elasticity, with a compressive modulus comparable to human skin. This demonstrates the potential of slide-ring hydrogels for fabricating robust, label-free biosensing platforms. Furthermore, the flexibility of this system allows for the incorporation of various recognition elements tailored to specific applications.https://www.mdpi.com/2310-2861/11/6/415hydrogellabel freeoptical biosensingslide ringsholographysurface relief grating |
| spellingShingle | Aitor Cubells-Gómez María Isabel Lucío María-José Bañuls Ángel Maquieira Surface Relief Gratings of Slide-Ring Hydrogels for Label-Free Biosensing Gels hydrogel label free optical biosensing slide rings holography surface relief grating |
| title | Surface Relief Gratings of Slide-Ring Hydrogels for Label-Free Biosensing |
| title_full | Surface Relief Gratings of Slide-Ring Hydrogels for Label-Free Biosensing |
| title_fullStr | Surface Relief Gratings of Slide-Ring Hydrogels for Label-Free Biosensing |
| title_full_unstemmed | Surface Relief Gratings of Slide-Ring Hydrogels for Label-Free Biosensing |
| title_short | Surface Relief Gratings of Slide-Ring Hydrogels for Label-Free Biosensing |
| title_sort | surface relief gratings of slide ring hydrogels for label free biosensing |
| topic | hydrogel label free optical biosensing slide rings holography surface relief grating |
| url | https://www.mdpi.com/2310-2861/11/6/415 |
| work_keys_str_mv | AT aitorcubellsgomez surfacereliefgratingsofslideringhydrogelsforlabelfreebiosensing AT mariaisabellucio surfacereliefgratingsofslideringhydrogelsforlabelfreebiosensing AT mariajosebanuls surfacereliefgratingsofslideringhydrogelsforlabelfreebiosensing AT angelmaquieira surfacereliefgratingsofslideringhydrogelsforlabelfreebiosensing |