A fluorescence probe for monitoring toxic hypochlorous acid in biosystems and environmental waters with a broad pH adaptation
Hypochlorous acid (HOCl) is commonly utilized in various daily applications. As a toxic reactive oxygen species (ROS) that generated from the industrial and pharmaceutical aspects, it plays a critical role in the mass cycle of environmental system and various biological processes. Understanding the...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324014465 |
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| author | Junyan Ma Zilin Jiao Mingtao Zhao Xiangtao Kong Hua Xie Zhenxing Zhang |
| author_facet | Junyan Ma Zilin Jiao Mingtao Zhao Xiangtao Kong Hua Xie Zhenxing Zhang |
| author_sort | Junyan Ma |
| collection | DOAJ |
| description | Hypochlorous acid (HOCl) is commonly utilized in various daily applications. As a toxic reactive oxygen species (ROS) that generated from the industrial and pharmaceutical aspects, it plays a critical role in the mass cycle of environmental system and various biological processes. Understanding the complicated roles of HOCl in environment and biosystems requires the development of precise and efficient detection methods. Thus, in this work, a novel fluorescent probe, MQ-ClO, has been designed to detect hypochlorous acid by utilizing hypochlorous acid-triggered oxidative intramolecular cyclization. This probe exhibits rapid and sensitive response, with a detection limit as low as 35 nM. More importantly, the probe is capable of functioning under highly acidic or basic conditions, exhibiting a wide pH range adaptability from pH 2–11. Furthermore, MQ-ClO has been effectively used to detect HOCl in real water samples. Besides, the low toxicity of MQ-ClO enables its practical application in monitoring endogenous/exogenous HOCl levels in living cells as well as zebrafish. |
| format | Article |
| id | doaj-art-457ac31309b34515829ba2ea534e08ac |
| institution | OA Journals |
| issn | 0147-6513 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-457ac31309b34515829ba2ea534e08ac2025-08-20T02:21:04ZengElsevierEcotoxicology and Environmental Safety0147-65132024-12-0128811737010.1016/j.ecoenv.2024.117370A fluorescence probe for monitoring toxic hypochlorous acid in biosystems and environmental waters with a broad pH adaptationJunyan Ma0Zilin Jiao1Mingtao Zhao2Xiangtao Kong3Hua Xie4Zhenxing Zhang5State Key Laboratory of New Optical Functional Materials, Anyang Normal University, Anyang, Henan 455000, China; Department of Chemistry, Clemson University, Clemson, SC 29634, United States; Corresponding authors at: State Key Laboratory of New Optical Functional Materials, Anyang Normal University, Anyang, Henan 455000, China.State Key Laboratory of New Optical Functional Materials, Anyang Normal University, Anyang, Henan 455000, ChinaState Key Laboratory of New Optical Functional Materials, Anyang Normal University, Anyang, Henan 455000, ChinaState Key Laboratory of New Optical Functional Materials, Anyang Normal University, Anyang, Henan 455000, ChinaState Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaState Key Laboratory of New Optical Functional Materials, Anyang Normal University, Anyang, Henan 455000, China; Department of Energy and Resource Engineering, College of Engineering, Peking University, Beijing 100871, China; Corresponding authors at: State Key Laboratory of New Optical Functional Materials, Anyang Normal University, Anyang, Henan 455000, China.Hypochlorous acid (HOCl) is commonly utilized in various daily applications. As a toxic reactive oxygen species (ROS) that generated from the industrial and pharmaceutical aspects, it plays a critical role in the mass cycle of environmental system and various biological processes. Understanding the complicated roles of HOCl in environment and biosystems requires the development of precise and efficient detection methods. Thus, in this work, a novel fluorescent probe, MQ-ClO, has been designed to detect hypochlorous acid by utilizing hypochlorous acid-triggered oxidative intramolecular cyclization. This probe exhibits rapid and sensitive response, with a detection limit as low as 35 nM. More importantly, the probe is capable of functioning under highly acidic or basic conditions, exhibiting a wide pH range adaptability from pH 2–11. Furthermore, MQ-ClO has been effectively used to detect HOCl in real water samples. Besides, the low toxicity of MQ-ClO enables its practical application in monitoring endogenous/exogenous HOCl levels in living cells as well as zebrafish.http://www.sciencedirect.com/science/article/pii/S0147651324014465Hypochlorous acidFluorescence probeDetectImagingWater samples |
| spellingShingle | Junyan Ma Zilin Jiao Mingtao Zhao Xiangtao Kong Hua Xie Zhenxing Zhang A fluorescence probe for monitoring toxic hypochlorous acid in biosystems and environmental waters with a broad pH adaptation Ecotoxicology and Environmental Safety Hypochlorous acid Fluorescence probe Detect Imaging Water samples |
| title | A fluorescence probe for monitoring toxic hypochlorous acid in biosystems and environmental waters with a broad pH adaptation |
| title_full | A fluorescence probe for monitoring toxic hypochlorous acid in biosystems and environmental waters with a broad pH adaptation |
| title_fullStr | A fluorescence probe for monitoring toxic hypochlorous acid in biosystems and environmental waters with a broad pH adaptation |
| title_full_unstemmed | A fluorescence probe for monitoring toxic hypochlorous acid in biosystems and environmental waters with a broad pH adaptation |
| title_short | A fluorescence probe for monitoring toxic hypochlorous acid in biosystems and environmental waters with a broad pH adaptation |
| title_sort | fluorescence probe for monitoring toxic hypochlorous acid in biosystems and environmental waters with a broad ph adaptation |
| topic | Hypochlorous acid Fluorescence probe Detect Imaging Water samples |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324014465 |
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