CBC Complex Regulates Hyphal Growth, Sclerotial Quantity, and Pathogenicity in the Necrotrophic Fungus <i>Botrytis cinerea</i>
The cap-binding protein complex (CBC), comprising Cbp20 and Cbp80, is crucial for gene expression, yet its role in the notorious crop pathogen <i>Botrytis cinerea</i> remains unclear. Immunoprecipitation coupled with LC-MS/MS demonstrated that BcCbp20 interacts with BcCbp80. Yeast two-hy...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/11/6/429 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849432493494632448 |
|---|---|
| author | Yinshan Zhang Xueting Chen Guihua Li Qingming Qin Mingzhe Zhang Jianchun Qin |
| author_facet | Yinshan Zhang Xueting Chen Guihua Li Qingming Qin Mingzhe Zhang Jianchun Qin |
| author_sort | Yinshan Zhang |
| collection | DOAJ |
| description | The cap-binding protein complex (CBC), comprising Cbp20 and Cbp80, is crucial for gene expression, yet its role in the notorious crop pathogen <i>Botrytis cinerea</i> remains unclear. Immunoprecipitation coupled with LC-MS/MS demonstrated that BcCbp20 interacts with BcCbp80. Yeast two-hybrid, GST pull-down, and Split-luciferase complementation assays confirmed that the conserved RNA recognition motif (RRM, 54–127 aa) of BcCbp20 and the N-terminal MIF4G domain (1–370 aa, 1–577 aa) of BcCbp80 constitute the core interaction regions. Genetic transformation experiments revealed that <i>BcCBP80</i> exerts a more dominant role than <i>BcCBP20</i> in regulating hyphal morphology, growth rate, conidiophore development, and conidial yield. Furthermore, <i>BcCBP20</i> and <i>BcCBP80</i> differentially regulate sclerotium formation to maintain sclerotial quantity. Based on pathogenicity assays, <i>BcCBP80</i> associated with infection cushion development, with this phenotypic alteration possibly being among the factors correlated with altered pathogenicity. However, the increased sensitivity of Δ<i>Bccbp20</i> to various stress factors may be the primary reason for the diminished pathogenicity. Taken together, these results indicate that <i>BcCBP20</i> and <i>BcCBP80</i> play important roles in multiple aspects of <i>B. cinerea</i> growth, development, stress response, and pathogenicity. |
| format | Article |
| id | doaj-art-4575460a4e514311b85ac03548902a41 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-4575460a4e514311b85ac03548902a412025-08-20T03:27:21ZengMDPI AGJournal of Fungi2309-608X2025-06-0111642910.3390/jof11060429CBC Complex Regulates Hyphal Growth, Sclerotial Quantity, and Pathogenicity in the Necrotrophic Fungus <i>Botrytis cinerea</i>Yinshan Zhang0Xueting Chen1Guihua Li2Qingming Qin3Mingzhe Zhang4Jianchun Qin5College of Plant Science, Jilin University, Changchun 130062, ChinaCollege of Plant Science, Jilin University, Changchun 130062, ChinaCollege of Plant Science, Jilin University, Changchun 130062, ChinaCollege of Plant Science, Jilin University, Changchun 130062, ChinaCollege of Plant Science, Jilin University, Changchun 130062, ChinaCollege of Plant Science, Jilin University, Changchun 130062, ChinaThe cap-binding protein complex (CBC), comprising Cbp20 and Cbp80, is crucial for gene expression, yet its role in the notorious crop pathogen <i>Botrytis cinerea</i> remains unclear. Immunoprecipitation coupled with LC-MS/MS demonstrated that BcCbp20 interacts with BcCbp80. Yeast two-hybrid, GST pull-down, and Split-luciferase complementation assays confirmed that the conserved RNA recognition motif (RRM, 54–127 aa) of BcCbp20 and the N-terminal MIF4G domain (1–370 aa, 1–577 aa) of BcCbp80 constitute the core interaction regions. Genetic transformation experiments revealed that <i>BcCBP80</i> exerts a more dominant role than <i>BcCBP20</i> in regulating hyphal morphology, growth rate, conidiophore development, and conidial yield. Furthermore, <i>BcCBP20</i> and <i>BcCBP80</i> differentially regulate sclerotium formation to maintain sclerotial quantity. Based on pathogenicity assays, <i>BcCBP80</i> associated with infection cushion development, with this phenotypic alteration possibly being among the factors correlated with altered pathogenicity. However, the increased sensitivity of Δ<i>Bccbp20</i> to various stress factors may be the primary reason for the diminished pathogenicity. Taken together, these results indicate that <i>BcCBP20</i> and <i>BcCBP80</i> play important roles in multiple aspects of <i>B. cinerea</i> growth, development, stress response, and pathogenicity.https://www.mdpi.com/2309-608X/11/6/429cap-binding protein complex<i>BcCBP20</i><i>BcCBP80</i>RRMMIF4Ginfection cushion |
| spellingShingle | Yinshan Zhang Xueting Chen Guihua Li Qingming Qin Mingzhe Zhang Jianchun Qin CBC Complex Regulates Hyphal Growth, Sclerotial Quantity, and Pathogenicity in the Necrotrophic Fungus <i>Botrytis cinerea</i> Journal of Fungi cap-binding protein complex <i>BcCBP20</i> <i>BcCBP80</i> RRM MIF4G infection cushion |
| title | CBC Complex Regulates Hyphal Growth, Sclerotial Quantity, and Pathogenicity in the Necrotrophic Fungus <i>Botrytis cinerea</i> |
| title_full | CBC Complex Regulates Hyphal Growth, Sclerotial Quantity, and Pathogenicity in the Necrotrophic Fungus <i>Botrytis cinerea</i> |
| title_fullStr | CBC Complex Regulates Hyphal Growth, Sclerotial Quantity, and Pathogenicity in the Necrotrophic Fungus <i>Botrytis cinerea</i> |
| title_full_unstemmed | CBC Complex Regulates Hyphal Growth, Sclerotial Quantity, and Pathogenicity in the Necrotrophic Fungus <i>Botrytis cinerea</i> |
| title_short | CBC Complex Regulates Hyphal Growth, Sclerotial Quantity, and Pathogenicity in the Necrotrophic Fungus <i>Botrytis cinerea</i> |
| title_sort | cbc complex regulates hyphal growth sclerotial quantity and pathogenicity in the necrotrophic fungus i botrytis cinerea i |
| topic | cap-binding protein complex <i>BcCBP20</i> <i>BcCBP80</i> RRM MIF4G infection cushion |
| url | https://www.mdpi.com/2309-608X/11/6/429 |
| work_keys_str_mv | AT yinshanzhang cbccomplexregulateshyphalgrowthsclerotialquantityandpathogenicityinthenecrotrophicfungusibotrytiscinereai AT xuetingchen cbccomplexregulateshyphalgrowthsclerotialquantityandpathogenicityinthenecrotrophicfungusibotrytiscinereai AT guihuali cbccomplexregulateshyphalgrowthsclerotialquantityandpathogenicityinthenecrotrophicfungusibotrytiscinereai AT qingmingqin cbccomplexregulateshyphalgrowthsclerotialquantityandpathogenicityinthenecrotrophicfungusibotrytiscinereai AT mingzhezhang cbccomplexregulateshyphalgrowthsclerotialquantityandpathogenicityinthenecrotrophicfungusibotrytiscinereai AT jianchunqin cbccomplexregulateshyphalgrowthsclerotialquantityandpathogenicityinthenecrotrophicfungusibotrytiscinereai |