Advanced capabilities for in vitro stress diagnostics: a review

INTRODUCTION. The effects of stress negatively affect both physical and mental health. Recently, there has been a sharp increase in interest in studying evidence-based methods for diagnosing stress and effective interventions for its correction. AIM. Comprehensive assessment of modern possibiliti...

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Main Authors: Yana G. Pekhova, Anna A. Kuzyukova, Larisa A. Marchenkova
Format: Article
Language:English
Published: Ministry of Health of the Russian Federation. National Medical Research Center of Rehabilitation and Balneology 2025-02-01
Series:Вестник восстановительной медицины
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Online Access:https://journals.eco-vector.com/2078-1962/article/viewFile/641812/pdf
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author Yana G. Pekhova
Anna A. Kuzyukova
Larisa A. Marchenkova
author_facet Yana G. Pekhova
Anna A. Kuzyukova
Larisa A. Marchenkova
author_sort Yana G. Pekhova
collection DOAJ
description INTRODUCTION. The effects of stress negatively affect both physical and mental health. Recently, there has been a sharp increase in interest in studying evidence-based methods for diagnosing stress and effective interventions for its correction. AIM. Comprehensive assessment of modern possibilities of stress objectification using laboratory diagnostics of its markers based on data from literary sources. MATERIALS AND METHODS. To select publications, we studied the PubMed, Web of Science (Web of Science Core Collection and Medline), Cochrane Library databases, included data from meta-analyses and review articles, full-text articles investigating the relationship between laboratory biomarkers and stress. The search depth of publications was 10 years, from 2014 to 2024, and a number of earlier, fundamental works on the neurophysiology of stress were also included in the review. RESULTS AND DISCUSSION. The biological basis of stress manifestations is a growing area of interest for scientists, as evidenced by the increasing number of publications on cortisol and dehydroepiandrosterone (DHEA) over the past 10 years. The review reflects a modern view on the role of laboratory markers in stress diagnostics. Biomarkers such as cortisol (the level of the surge after awakening, the average level of cortisol during the day, the daily curve of cortisol, cortisol in the hair), salivary alpha-amylase, DHEA, etc. are described, information on their sensitivity and specificity in the verification of acute and chronic stress conditions is analyzed. CONCLUSION. Cortisol is the most reliable and frequently used laboratory marker of both acute and chronic stress, while the study of other potential biomarkers continues to grow. Psychometric questionnaires and functional diagnostic methods reflecting the degree of sympathetic activation are widely used in stress diagnostics. The integrated use of various diagnostic tools, including laboratory biomarkers of stress, will provide a multimodal approach, will contribute to a more complete picture of the stress response and will increase the degree of verification of stress conditions.
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spelling doaj-art-457489d5b01b4cd2a582931279943ccd2025-08-20T03:42:38ZengMinistry of Health of the Russian Federation. National Medical Research Center of Rehabilitation and BalneologyВестник восстановительной медицины2078-19622713-26252025-02-01241677410.38025/2078-1962-2025-24-1-67-741459Advanced capabilities for in vitro stress diagnostics: a reviewYana G. Pekhova0https://orcid.org/0000-0002-2754-1021Anna A. Kuzyukova1https://orcid.org/0000-0002-9275-6491Larisa A. Marchenkova2https://orcid.org/0000-0003-1886-124XNational Medical Research Center for Rehabilitation and BalneologyNational Medical Research Center for Rehabilitation and BalneologyNational Medical Research Center for Rehabilitation and BalneologyINTRODUCTION. The effects of stress negatively affect both physical and mental health. Recently, there has been a sharp increase in interest in studying evidence-based methods for diagnosing stress and effective interventions for its correction. AIM. Comprehensive assessment of modern possibilities of stress objectification using laboratory diagnostics of its markers based on data from literary sources. MATERIALS AND METHODS. To select publications, we studied the PubMed, Web of Science (Web of Science Core Collection and Medline), Cochrane Library databases, included data from meta-analyses and review articles, full-text articles investigating the relationship between laboratory biomarkers and stress. The search depth of publications was 10 years, from 2014 to 2024, and a number of earlier, fundamental works on the neurophysiology of stress were also included in the review. RESULTS AND DISCUSSION. The biological basis of stress manifestations is a growing area of interest for scientists, as evidenced by the increasing number of publications on cortisol and dehydroepiandrosterone (DHEA) over the past 10 years. The review reflects a modern view on the role of laboratory markers in stress diagnostics. Biomarkers such as cortisol (the level of the surge after awakening, the average level of cortisol during the day, the daily curve of cortisol, cortisol in the hair), salivary alpha-amylase, DHEA, etc. are described, information on their sensitivity and specificity in the verification of acute and chronic stress conditions is analyzed. CONCLUSION. Cortisol is the most reliable and frequently used laboratory marker of both acute and chronic stress, while the study of other potential biomarkers continues to grow. Psychometric questionnaires and functional diagnostic methods reflecting the degree of sympathetic activation are widely used in stress diagnostics. The integrated use of various diagnostic tools, including laboratory biomarkers of stress, will provide a multimodal approach, will contribute to a more complete picture of the stress response and will increase the degree of verification of stress conditions.https://journals.eco-vector.com/2078-1962/article/viewFile/641812/pdfstresschronic stressbiomarkerscortisolsalivary cortisolurinary cortisolhair cortisolsalivary alpha-amylasedehydroepiandrosteronesympathetic-adrenal systemhypothalamic-pituitary-adrenal axis
spellingShingle Yana G. Pekhova
Anna A. Kuzyukova
Larisa A. Marchenkova
Advanced capabilities for in vitro stress diagnostics: a review
Вестник восстановительной медицины
stress
chronic stress
biomarkers
cortisol
salivary cortisol
urinary cortisol
hair cortisol
salivary alpha-amylase
dehydroepiandrosterone
sympathetic-adrenal system
hypothalamic-pituitary-adrenal axis
title Advanced capabilities for in vitro stress diagnostics: a review
title_full Advanced capabilities for in vitro stress diagnostics: a review
title_fullStr Advanced capabilities for in vitro stress diagnostics: a review
title_full_unstemmed Advanced capabilities for in vitro stress diagnostics: a review
title_short Advanced capabilities for in vitro stress diagnostics: a review
title_sort advanced capabilities for in vitro stress diagnostics a review
topic stress
chronic stress
biomarkers
cortisol
salivary cortisol
urinary cortisol
hair cortisol
salivary alpha-amylase
dehydroepiandrosterone
sympathetic-adrenal system
hypothalamic-pituitary-adrenal axis
url https://journals.eco-vector.com/2078-1962/article/viewFile/641812/pdf
work_keys_str_mv AT yanagpekhova advancedcapabilitiesforinvitrostressdiagnosticsareview
AT annaakuzyukova advancedcapabilitiesforinvitrostressdiagnosticsareview
AT larisaamarchenkova advancedcapabilitiesforinvitrostressdiagnosticsareview