Direct Amplification of the Entire ITS Region from Poorly Preserved Plant Material Using Recombinant PCR
Sequences of the internal transcribed spacers (ITS) of the nuclear ribosomal DNA are important molecular markers in phylogenetic analyses. To obtain sequences from herbarium material in which DNA often is severely degraded, the ITS region has to be amplified in two steps. Two methods that reduce ben...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1999-12-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/99276st04 |
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