Cystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in Mycobacterium abscessus
Abstract Mycobacterium abscessus complex (MABSC) comprises a group of environmental microorganisms, which are a concerning cause of opportunistic respiratory infections in patients with cystic fibrosis or bronchiectasis. Only 45.6% of MABSC treatments are successful, and therefore this is a need to...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-11-01
|
| Series: | npj Antimicrobials and Resistance |
| Online Access: | https://doi.org/10.1038/s44259-024-00054-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850062026475307008 |
|---|---|
| author | Emily J. Baker Gemma Allcott Antonia Molloy Jonathan A. G. Cox |
| author_facet | Emily J. Baker Gemma Allcott Antonia Molloy Jonathan A. G. Cox |
| author_sort | Emily J. Baker |
| collection | DOAJ |
| description | Abstract Mycobacterium abscessus complex (MABSC) comprises a group of environmental microorganisms, which are a concerning cause of opportunistic respiratory infections in patients with cystic fibrosis or bronchiectasis. Only 45.6% of MABSC treatments are successful, and therefore this is a need to discover new antimicrobials that can treat these pathogens. However, the transferability of outcomes to the clinic is flawed by an inability to accurately represent the lung environment within the laboratory. Herein, we apply two preestablished formulations of sputum media (ACFS and SCFM1) to MABSC antibiotic susceptibility testing. Using conventional broth microdilution, we have observed strain and antibiotic dependent alterations in antimicrobial sensitivity in each sputum media compared standard laboratory media (7H9), with an overall reduction in susceptibility within the physiologically relevant conditions. We provide a timely contribution to the field of M. abscessus antibiotic discovery by emphasising the need for improved physiological relevance. |
| format | Article |
| id | doaj-art-45693e7559ab4d60be972d92b6a1b48e |
| institution | DOAJ |
| issn | 2731-8745 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Antimicrobials and Resistance |
| spelling | doaj-art-45693e7559ab4d60be972d92b6a1b48e2025-08-20T02:50:02ZengNature Portfolionpj Antimicrobials and Resistance2731-87452024-11-01211810.1038/s44259-024-00054-3Cystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in Mycobacterium abscessusEmily J. Baker0Gemma Allcott1Antonia Molloy2Jonathan A. G. Cox3College of Health and Life Sciences, Aston University, Aston TriangleCollege of Health and Life Sciences, Aston University, Aston TriangleCollege of Health and Life Sciences, Aston University, Aston TriangleCollege of Health and Life Sciences, Aston University, Aston TriangleAbstract Mycobacterium abscessus complex (MABSC) comprises a group of environmental microorganisms, which are a concerning cause of opportunistic respiratory infections in patients with cystic fibrosis or bronchiectasis. Only 45.6% of MABSC treatments are successful, and therefore this is a need to discover new antimicrobials that can treat these pathogens. However, the transferability of outcomes to the clinic is flawed by an inability to accurately represent the lung environment within the laboratory. Herein, we apply two preestablished formulations of sputum media (ACFS and SCFM1) to MABSC antibiotic susceptibility testing. Using conventional broth microdilution, we have observed strain and antibiotic dependent alterations in antimicrobial sensitivity in each sputum media compared standard laboratory media (7H9), with an overall reduction in susceptibility within the physiologically relevant conditions. We provide a timely contribution to the field of M. abscessus antibiotic discovery by emphasising the need for improved physiological relevance.https://doi.org/10.1038/s44259-024-00054-3 |
| spellingShingle | Emily J. Baker Gemma Allcott Antonia Molloy Jonathan A. G. Cox Cystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in Mycobacterium abscessus npj Antimicrobials and Resistance |
| title | Cystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in Mycobacterium abscessus |
| title_full | Cystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in Mycobacterium abscessus |
| title_fullStr | Cystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in Mycobacterium abscessus |
| title_full_unstemmed | Cystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in Mycobacterium abscessus |
| title_short | Cystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in Mycobacterium abscessus |
| title_sort | cystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in mycobacterium abscessus |
| url | https://doi.org/10.1038/s44259-024-00054-3 |
| work_keys_str_mv | AT emilyjbaker cysticfibrosissputummediainducesanoveralllossofantibioticsusceptibilityinmycobacteriumabscessus AT gemmaallcott cysticfibrosissputummediainducesanoveralllossofantibioticsusceptibilityinmycobacteriumabscessus AT antoniamolloy cysticfibrosissputummediainducesanoveralllossofantibioticsusceptibilityinmycobacteriumabscessus AT jonathanagcox cysticfibrosissputummediainducesanoveralllossofantibioticsusceptibilityinmycobacteriumabscessus |