Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells
Induced pluripotent stem cells (iPSCs) have provided new opportunities for motor neuron disease (MND) modeling, drug screening, and cellular therapeutic development. Among the various types of iPSCs, urine-derived iPSCs have become a promising source of stem cells because they can be safely and noni...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/3628578 |
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| author | Huan Yi Bingbing Xie Ben Liu Xuan Wang Li Xu Jia Liu Min Li Xiufeng Zhong Fuhua Peng |
| author_facet | Huan Yi Bingbing Xie Ben Liu Xuan Wang Li Xu Jia Liu Min Li Xiufeng Zhong Fuhua Peng |
| author_sort | Huan Yi |
| collection | DOAJ |
| description | Induced pluripotent stem cells (iPSCs) have provided new opportunities for motor neuron disease (MND) modeling, drug screening, and cellular therapeutic development. Among the various types of iPSCs, urine-derived iPSCs have become a promising source of stem cells because they can be safely and noninvasively isolated and easily reprogrammed. Here, for the first time, we differentiated urine-derived iPSCs (urine-iPSCs) into motor neurons (MNs) and compared the capacity of urine-iPSCs and cord-blood-derived iPSCs (B-iPSCs) to differentiate into MNs. With the use of small molecules, mature MNs were generated from urine-iPSCs as early as 26 days in culture. Furthermore, in coculture with muscle cells, MNs projected long axons and formed neuromuscular junctions (NMJs). Immunofluorescence and PCR confirmed the expression levels of both MN and NMJ markers. The comparison of the ratios of positive labeling for MN markers between urine-iPSCs and B-iPSCs demonstrated that the differentiation potentials of these cells were not significantly different. The abovementioned results indicate that urine-iPSCs are a new, promising source of stem cells for MND modeling and further cellular therapeutic development. |
| format | Article |
| id | doaj-art-45582d0fe179435398199378a090aafe |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-45582d0fe179435398199378a090aafe2025-02-03T01:20:33ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/36285783628578Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem CellsHuan Yi0Bingbing Xie1Ben Liu2Xuan Wang3Li Xu4Jia Liu5Min Li6Xiufeng Zhong7Fuhua Peng8Department of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong 510600, ChinaDepartment of Dermatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, ChinaDepartment of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, ChinaDepartment of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, ChinaDepartment of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, ChinaDepartment of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, ChinaState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong 510600, ChinaDepartment of Neurology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, ChinaInduced pluripotent stem cells (iPSCs) have provided new opportunities for motor neuron disease (MND) modeling, drug screening, and cellular therapeutic development. Among the various types of iPSCs, urine-derived iPSCs have become a promising source of stem cells because they can be safely and noninvasively isolated and easily reprogrammed. Here, for the first time, we differentiated urine-derived iPSCs (urine-iPSCs) into motor neurons (MNs) and compared the capacity of urine-iPSCs and cord-blood-derived iPSCs (B-iPSCs) to differentiate into MNs. With the use of small molecules, mature MNs were generated from urine-iPSCs as early as 26 days in culture. Furthermore, in coculture with muscle cells, MNs projected long axons and formed neuromuscular junctions (NMJs). Immunofluorescence and PCR confirmed the expression levels of both MN and NMJ markers. The comparison of the ratios of positive labeling for MN markers between urine-iPSCs and B-iPSCs demonstrated that the differentiation potentials of these cells were not significantly different. The abovementioned results indicate that urine-iPSCs are a new, promising source of stem cells for MND modeling and further cellular therapeutic development.http://dx.doi.org/10.1155/2018/3628578 |
| spellingShingle | Huan Yi Bingbing Xie Ben Liu Xuan Wang Li Xu Jia Liu Min Li Xiufeng Zhong Fuhua Peng Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells Stem Cells International |
| title | Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells |
| title_full | Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells |
| title_fullStr | Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells |
| title_full_unstemmed | Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells |
| title_short | Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells |
| title_sort | derivation and identification of motor neurons from human urine derived induced pluripotent stem cells |
| url | http://dx.doi.org/10.1155/2018/3628578 |
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