First report of Pseudobodo sp, a new pathogen for a potential energy-producing algae: Chlorella vulgaris cultures.

Chlorella vulgaris, is a kind of single-celled green algae, which could serve as a potential source of food and energy because of its photosynthetic efficiency. In our study, a pathogenic organism targeting C. vulgaris was discovered. The algae-lytic activity relates to a fraction from lysates of in...

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Main Authors: Zhangran Chen, Xueqian Lei, Bangzhou Zhang, Luxi Yang, Huajun Zhang, Jingyan Zhang, Yi Li, Wei Zheng, Yun Tian, Jingwen Liu, Tianling Zheng
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089571&type=printable
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author Zhangran Chen
Xueqian Lei
Bangzhou Zhang
Luxi Yang
Huajun Zhang
Jingyan Zhang
Yi Li
Wei Zheng
Yun Tian
Jingwen Liu
Tianling Zheng
author_facet Zhangran Chen
Xueqian Lei
Bangzhou Zhang
Luxi Yang
Huajun Zhang
Jingyan Zhang
Yi Li
Wei Zheng
Yun Tian
Jingwen Liu
Tianling Zheng
author_sort Zhangran Chen
collection DOAJ
description Chlorella vulgaris, is a kind of single-celled green algae, which could serve as a potential source of food and energy because of its photosynthetic efficiency. In our study, a pathogenic organism targeting C. vulgaris was discovered. The algae-lytic activity relates to a fraction from lysates of infected C. vulgaris that was blocked upon filtration through a 3 µm filter. 18S rRNA gene sequence analysis revealed that it shared 99.0% homology with the protist Pseudobodo tremulans. Scanning electron microscope analysis showed that Pseudobodo sp. KD51 cells were approximately 4-5 µm long, biflagellate with an anterior collar around the anterior part of the cell in unstressed feeding cells. Besides the initial host, Pseudobodo sp. KD51 could also kill other algae, indicating its relatively wide predatory spectrum. Heat stability, pH and salinity tolerance experiments were conducted to understand their effects on its predatory activities, and the results showed that Pseudobodo sp. KD51 was heat-sensitive, and pH and salinity tolerant.
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institution DOAJ
issn 1932-6203
language English
publishDate 2014-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS ONE
spelling doaj-art-455689e98e6449a3bd1b288b26bea9af2025-08-20T03:11:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e8957110.1371/journal.pone.0089571First report of Pseudobodo sp, a new pathogen for a potential energy-producing algae: Chlorella vulgaris cultures.Zhangran ChenXueqian LeiBangzhou ZhangLuxi YangHuajun ZhangJingyan ZhangYi LiWei ZhengYun TianJingwen LiuTianling ZhengChlorella vulgaris, is a kind of single-celled green algae, which could serve as a potential source of food and energy because of its photosynthetic efficiency. In our study, a pathogenic organism targeting C. vulgaris was discovered. The algae-lytic activity relates to a fraction from lysates of infected C. vulgaris that was blocked upon filtration through a 3 µm filter. 18S rRNA gene sequence analysis revealed that it shared 99.0% homology with the protist Pseudobodo tremulans. Scanning electron microscope analysis showed that Pseudobodo sp. KD51 cells were approximately 4-5 µm long, biflagellate with an anterior collar around the anterior part of the cell in unstressed feeding cells. Besides the initial host, Pseudobodo sp. KD51 could also kill other algae, indicating its relatively wide predatory spectrum. Heat stability, pH and salinity tolerance experiments were conducted to understand their effects on its predatory activities, and the results showed that Pseudobodo sp. KD51 was heat-sensitive, and pH and salinity tolerant.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089571&type=printable
spellingShingle Zhangran Chen
Xueqian Lei
Bangzhou Zhang
Luxi Yang
Huajun Zhang
Jingyan Zhang
Yi Li
Wei Zheng
Yun Tian
Jingwen Liu
Tianling Zheng
First report of Pseudobodo sp, a new pathogen for a potential energy-producing algae: Chlorella vulgaris cultures.
PLoS ONE
title First report of Pseudobodo sp, a new pathogen for a potential energy-producing algae: Chlorella vulgaris cultures.
title_full First report of Pseudobodo sp, a new pathogen for a potential energy-producing algae: Chlorella vulgaris cultures.
title_fullStr First report of Pseudobodo sp, a new pathogen for a potential energy-producing algae: Chlorella vulgaris cultures.
title_full_unstemmed First report of Pseudobodo sp, a new pathogen for a potential energy-producing algae: Chlorella vulgaris cultures.
title_short First report of Pseudobodo sp, a new pathogen for a potential energy-producing algae: Chlorella vulgaris cultures.
title_sort first report of pseudobodo sp a new pathogen for a potential energy producing algae chlorella vulgaris cultures
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089571&type=printable
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