Detection of Hg2+ Using a Dual-Mode Biosensing Probe Constructed Using Ratiometric Fluorescent Copper Nanoclusters@Zirconia Metal-Organic Framework/N-Methyl Mesoporphyrin IX and Colorimetry G-Quadruplex/Hemin Peroxidase-Mimicking G-Quadruplex DNAzyme
Mercury (Hg2+) has been recognized as a global pollutant with a toxic, mobile, and persistent nature. It adversely affects the ecosystem and human health. Already developed biosensors for Hg2+ detection majorly suffer from poor sensitivity and specificity. Herein, a colorimetric/fluorimetric dual-mo...
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| Language: | English |
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American Association for the Advancement of Science (AAAS)
2024-01-01
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| Series: | BME Frontiers |
| Online Access: | https://spj.science.org/doi/10.34133/bmef.0078 |
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| author | Shikha Jain Monika Nehra Neeraj Dilbaghi Ganga Ram Chaudhary Sandeep Kumar |
| author_facet | Shikha Jain Monika Nehra Neeraj Dilbaghi Ganga Ram Chaudhary Sandeep Kumar |
| author_sort | Shikha Jain |
| collection | DOAJ |
| description | Mercury (Hg2+) has been recognized as a global pollutant with a toxic, mobile, and persistent nature. It adversely affects the ecosystem and human health. Already developed biosensors for Hg2+ detection majorly suffer from poor sensitivity and specificity. Herein, a colorimetric/fluorimetric dual-mode sensing approach is designed for the quantitative detection of Hg2+. This novel sensing approach utilizes nanofluorophores, i.e., fluorescent copper nanoclusters-doped zirconia metal-organic framework (CuNCs@Zr-MOF) nanoconjugate (blue color) and N-methyl mesoporphyrin IX (NMM) (red color) in combination with peroxidase-mimicking G-quadruplex DNAzyme (PMDNAzyme). In the presence of Hg2+, dabcyl conjugated complementary DNA with T–T mismatches form the stable duplex with the CuNCs@Zr-MOF@G-quadruplex structure through T–Hg2+–T base pairing. It causes the quenching of fluorescence of CuNCs@Zr-MOF (463 nm) due to the Förster resonance energy transfer (FRET) system. Moreover, the G-quadruplex (G4) structure of the aptamer enhances the fluorescence emission of NMM (610 nm). Besides this, the peroxidase-like activity of G4/hemin DNAzyme offers the colorimetric detection of Hg2+. The formation of duplex with PMDNAzyme increases the catalytic activity. This novel biosensing probe quantitatively detected Hg2+ using both fluorimetry and colorimetry approaches with a low detection limit of 0.59 and 36.3 nM, respectively. It was also observed that the presence of interfering metal ions in case of real aqueous samples does not affect the performance of this novel biosensing probe. These findings confirm the considerable potential of the proposed biosensing probe to screen the concentration of Hg2+ in aquatic products. |
| format | Article |
| id | doaj-art-454cdb0508714ec2a240112bb78f769e |
| institution | Kabale University |
| issn | 2765-8031 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | BME Frontiers |
| spelling | doaj-art-454cdb0508714ec2a240112bb78f769e2025-01-14T12:15:57ZengAmerican Association for the Advancement of Science (AAAS)BME Frontiers2765-80312024-01-01510.34133/bmef.0078Detection of Hg2+ Using a Dual-Mode Biosensing Probe Constructed Using Ratiometric Fluorescent Copper Nanoclusters@Zirconia Metal-Organic Framework/N-Methyl Mesoporphyrin IX and Colorimetry G-Quadruplex/Hemin Peroxidase-Mimicking G-Quadruplex DNAzymeShikha Jain0Monika Nehra1Neeraj Dilbaghi2Ganga Ram Chaudhary3Sandeep Kumar4Department of Bio-nanotechnology, College of Biotechnology, CCS Haryana Agricultural University (CCSHAU), Hisar-Haryana 125004, India.Department of Mechanical Engineering, University Institute of Engineering and Technology, Panjab University, Chandigarh 160014, India.Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar-Haryana 125001, India.Department of Chemistry & Center of Advanced Studies in Chemistry, Panjab University, Chandigarh 160014, India.Department of Physics, Punjab Engineering College (Deemed to be University), Chandigarh 160012, India.Mercury (Hg2+) has been recognized as a global pollutant with a toxic, mobile, and persistent nature. It adversely affects the ecosystem and human health. Already developed biosensors for Hg2+ detection majorly suffer from poor sensitivity and specificity. Herein, a colorimetric/fluorimetric dual-mode sensing approach is designed for the quantitative detection of Hg2+. This novel sensing approach utilizes nanofluorophores, i.e., fluorescent copper nanoclusters-doped zirconia metal-organic framework (CuNCs@Zr-MOF) nanoconjugate (blue color) and N-methyl mesoporphyrin IX (NMM) (red color) in combination with peroxidase-mimicking G-quadruplex DNAzyme (PMDNAzyme). In the presence of Hg2+, dabcyl conjugated complementary DNA with T–T mismatches form the stable duplex with the CuNCs@Zr-MOF@G-quadruplex structure through T–Hg2+–T base pairing. It causes the quenching of fluorescence of CuNCs@Zr-MOF (463 nm) due to the Förster resonance energy transfer (FRET) system. Moreover, the G-quadruplex (G4) structure of the aptamer enhances the fluorescence emission of NMM (610 nm). Besides this, the peroxidase-like activity of G4/hemin DNAzyme offers the colorimetric detection of Hg2+. The formation of duplex with PMDNAzyme increases the catalytic activity. This novel biosensing probe quantitatively detected Hg2+ using both fluorimetry and colorimetry approaches with a low detection limit of 0.59 and 36.3 nM, respectively. It was also observed that the presence of interfering metal ions in case of real aqueous samples does not affect the performance of this novel biosensing probe. These findings confirm the considerable potential of the proposed biosensing probe to screen the concentration of Hg2+ in aquatic products.https://spj.science.org/doi/10.34133/bmef.0078 |
| spellingShingle | Shikha Jain Monika Nehra Neeraj Dilbaghi Ganga Ram Chaudhary Sandeep Kumar Detection of Hg2+ Using a Dual-Mode Biosensing Probe Constructed Using Ratiometric Fluorescent Copper Nanoclusters@Zirconia Metal-Organic Framework/N-Methyl Mesoporphyrin IX and Colorimetry G-Quadruplex/Hemin Peroxidase-Mimicking G-Quadruplex DNAzyme BME Frontiers |
| title | Detection of Hg2+ Using a Dual-Mode Biosensing Probe Constructed Using Ratiometric Fluorescent Copper Nanoclusters@Zirconia Metal-Organic Framework/N-Methyl Mesoporphyrin IX and Colorimetry G-Quadruplex/Hemin Peroxidase-Mimicking G-Quadruplex DNAzyme |
| title_full | Detection of Hg2+ Using a Dual-Mode Biosensing Probe Constructed Using Ratiometric Fluorescent Copper Nanoclusters@Zirconia Metal-Organic Framework/N-Methyl Mesoporphyrin IX and Colorimetry G-Quadruplex/Hemin Peroxidase-Mimicking G-Quadruplex DNAzyme |
| title_fullStr | Detection of Hg2+ Using a Dual-Mode Biosensing Probe Constructed Using Ratiometric Fluorescent Copper Nanoclusters@Zirconia Metal-Organic Framework/N-Methyl Mesoporphyrin IX and Colorimetry G-Quadruplex/Hemin Peroxidase-Mimicking G-Quadruplex DNAzyme |
| title_full_unstemmed | Detection of Hg2+ Using a Dual-Mode Biosensing Probe Constructed Using Ratiometric Fluorescent Copper Nanoclusters@Zirconia Metal-Organic Framework/N-Methyl Mesoporphyrin IX and Colorimetry G-Quadruplex/Hemin Peroxidase-Mimicking G-Quadruplex DNAzyme |
| title_short | Detection of Hg2+ Using a Dual-Mode Biosensing Probe Constructed Using Ratiometric Fluorescent Copper Nanoclusters@Zirconia Metal-Organic Framework/N-Methyl Mesoporphyrin IX and Colorimetry G-Quadruplex/Hemin Peroxidase-Mimicking G-Quadruplex DNAzyme |
| title_sort | detection of hg2 using a dual mode biosensing probe constructed using ratiometric fluorescent copper nanoclusters zirconia metal organic framework n methyl mesoporphyrin ix and colorimetry g quadruplex hemin peroxidase mimicking g quadruplex dnazyme |
| url | https://spj.science.org/doi/10.34133/bmef.0078 |
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