An approach to the meso-scale epidemiological behavior of Plasmodiophora brassicae from cruciferous crops under tropical conditions; Supplementary material

Plasmodiophora brassicae is an obligate parasite and a natural soil inhabitant that causes clubroot, a disease with significant economic impact in plants of the Brassicaceae family. This pathology is conditioned by plant/host interactions, edaphoclimatic variables, and mechanisms of inoculum dispers...

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Main Authors: Andres F. Quintero-Mercado, Juan Camilo Garcia-Peña, Andrea Botero-Ramirez, Celsa Garcia, Joaquín Guillermo Ramírez-Gil
Format: Article
Language:English
Published: Polish Academy of Sciences 2025-07-01
Series:Journal of Plant Protection Research
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Online Access:https://journals.pan.pl/Content/135779/PDF/08_OA_JPPR_65_2_2125_Quintero-Mercado.pdf
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author Andres F. Quintero-Mercado
Juan Camilo Garcia-Peña
Andrea Botero-Ramirez
Celsa Garcia
Joaquín Guillermo Ramírez-Gil
author_facet Andres F. Quintero-Mercado
Juan Camilo Garcia-Peña
Andrea Botero-Ramirez
Celsa Garcia
Joaquín Guillermo Ramírez-Gil
author_sort Andres F. Quintero-Mercado
collection DOAJ
description Plasmodiophora brassicae is an obligate parasite and a natural soil inhabitant that causes clubroot, a disease with significant economic impact in plants of the Brassicaceae family. This pathology is conditioned by plant/host interactions, edaphoclimatic variables, and mechanisms of inoculum dispersal. However, the epidemiology of this pathogen is not well understood, thereby limiting its incorporation into integrated disease management strategies (IDM). The objective of this work was to adjust a mesoscale risk and prognostic model of P. brassicae based on edaphoclimatic factors and potential dispersal mechanisms in brassica-producing areas in Colombia. The presence and inoculum density of the pathogen were determined by visual inspection of symptoms and quantification by qPCR of soil samples in a total of 127 plots located in regions with the highest production of species from the Brassicaceae family. In addition, an edaphoclimatic characterization was carried out based on field data and secondary information by web scraping using freely available databases. The forecast models were determined by fitting a Generalized Linear Model (GLM) using the logit and inverse link functions for binomial and gamma distributions, respectively. The meso- and macroscale spatial risk model was developed under point pattern approaches (Kernel density model and ecological niche model (ENM). The different epidemiological analysis approaches used suggest that P. brassicae presents a high risk in areas with host presence and conducive edaphoclimatic characteristics, indicating the need to carry out epidemiological surveillance, reduce the dispersion of infested soil, and implement P. brassicae exclusion methods.
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institution Kabale University
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publishDate 2025-07-01
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record_format Article
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spelling doaj-art-453c15792f724f02a290f5d426863dc72025-08-20T03:25:22ZengPolish Academy of SciencesJournal of Plant Protection Research1427-43451899-007X2025-07-01vol. 65No 2https://doi.org/10.24425/jppr.2025.155057An approach to the meso-scale epidemiological behavior of Plasmodiophora brassicae from cruciferous crops under tropical conditions; Supplementary materialAndres F. Quintero-Mercado0https://orcid.org/0000-0002-9907-3490Juan Camilo Garcia-Peña1https://orcid.org/0000-0003-3423-1416Andrea Botero-Ramirez2https://orcid.org/0000-0002-0162-3598Celsa Garcia3Joaquín Guillermo Ramírez-Gil4Universidad del Magdalena, Facultad de Ingeniería, Santa Marta, ColombiaDepartamento de Agronomía, Facultad de Ciencias Agrarias, Universidad Nacional de Colombia, Sede Bogotá, 111321, Bogotá, ColombiaPlant Growth Facility Lead, Faculty of Agricultural, Life, and Environmental Sciences, 5-17 Ag/Forestry Building University of Alberta, CanadaDepartamento de Agronomía, Facultad de Ciencias Agrarias, Universidad Nacional de Colombia, Sede Bogotá, 111321, Bogotá, ColombiaDepartamento de Agronomía, Facultad de Ciencias Agrarias, Universidad Nacional de Colombia, Sede Bogotá, 111321, Bogotá, ColombiaPlasmodiophora brassicae is an obligate parasite and a natural soil inhabitant that causes clubroot, a disease with significant economic impact in plants of the Brassicaceae family. This pathology is conditioned by plant/host interactions, edaphoclimatic variables, and mechanisms of inoculum dispersal. However, the epidemiology of this pathogen is not well understood, thereby limiting its incorporation into integrated disease management strategies (IDM). The objective of this work was to adjust a mesoscale risk and prognostic model of P. brassicae based on edaphoclimatic factors and potential dispersal mechanisms in brassica-producing areas in Colombia. The presence and inoculum density of the pathogen were determined by visual inspection of symptoms and quantification by qPCR of soil samples in a total of 127 plots located in regions with the highest production of species from the Brassicaceae family. In addition, an edaphoclimatic characterization was carried out based on field data and secondary information by web scraping using freely available databases. The forecast models were determined by fitting a Generalized Linear Model (GLM) using the logit and inverse link functions for binomial and gamma distributions, respectively. The meso- and macroscale spatial risk model was developed under point pattern approaches (Kernel density model and ecological niche model (ENM). The different epidemiological analysis approaches used suggest that P. brassicae presents a high risk in areas with host presence and conducive edaphoclimatic characteristics, indicating the need to carry out epidemiological surveillance, reduce the dispersion of infested soil, and implement P. brassicae exclusion methods.https://journals.pan.pl/Content/135779/PDF/08_OA_JPPR_65_2_2125_Quintero-Mercado.pdfclubrootdriving factorsecological niche modelgeneralized linear modelrisk analysis
spellingShingle Andres F. Quintero-Mercado
Juan Camilo Garcia-Peña
Andrea Botero-Ramirez
Celsa Garcia
Joaquín Guillermo Ramírez-Gil
An approach to the meso-scale epidemiological behavior of Plasmodiophora brassicae from cruciferous crops under tropical conditions; Supplementary material
Journal of Plant Protection Research
clubroot
driving factors
ecological niche model
generalized linear model
risk analysis
title An approach to the meso-scale epidemiological behavior of Plasmodiophora brassicae from cruciferous crops under tropical conditions; Supplementary material
title_full An approach to the meso-scale epidemiological behavior of Plasmodiophora brassicae from cruciferous crops under tropical conditions; Supplementary material
title_fullStr An approach to the meso-scale epidemiological behavior of Plasmodiophora brassicae from cruciferous crops under tropical conditions; Supplementary material
title_full_unstemmed An approach to the meso-scale epidemiological behavior of Plasmodiophora brassicae from cruciferous crops under tropical conditions; Supplementary material
title_short An approach to the meso-scale epidemiological behavior of Plasmodiophora brassicae from cruciferous crops under tropical conditions; Supplementary material
title_sort approach to the meso scale epidemiological behavior of plasmodiophora brassicae from cruciferous crops under tropical conditions supplementary material
topic clubroot
driving factors
ecological niche model
generalized linear model
risk analysis
url https://journals.pan.pl/Content/135779/PDF/08_OA_JPPR_65_2_2125_Quintero-Mercado.pdf
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