Discovery and mechanism of anti-hypertensive effect of a novel tripeptide (SYP) from medicinal fungus Ganoderma lingzhi
Ganoderma lingzhi is a new species of the prize medicinal mushroom Ganoderma (Agaricomycetes). Using angiotensin Ⅰ-converting enzyme (ACE) as a target, a tripeptide Ser-Tyr-Pro (SYP) was discovered with preponderant ACE inhibitory activity with an 50% inhibiting concentration (IC50) value of 62.50 μ...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2025-02-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2024.9250032 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850225556161822720 |
|---|---|
| author | Qiang Wu Feijun Luo Jia Huang Ping Huang Yongnan Liu Xiaoling Wang Gaoqiang Liu |
| author_facet | Qiang Wu Feijun Luo Jia Huang Ping Huang Yongnan Liu Xiaoling Wang Gaoqiang Liu |
| author_sort | Qiang Wu |
| collection | DOAJ |
| description | Ganoderma lingzhi is a new species of the prize medicinal mushroom Ganoderma (Agaricomycetes). Using angiotensin Ⅰ-converting enzyme (ACE) as a target, a tripeptide Ser-Tyr-Pro (SYP) was discovered with preponderant ACE inhibitory activity with an 50% inhibiting concentration (IC50) value of 62.50 μg/mL attribute to the formed salt bridge and hydrogen bonds between SYP and ACE. SYP even maintained superior bioactivity after intestinal digestion, and exerted no cytotoxicity, but presented incomplete bioavailability in blood of spontaneous hypertensive rats (SHRs). Furthermore, it performed antihypertensive effect in vivo by inhibiting the influx of Ca2+ through activating endothelial NO synthase (eNOS)/NO/guanosine 3’,5’-cyclic monophosphate (cGMP) pathway, accompanied by attenuating angiotensin Ⅱ (Ang Ⅱ)/NADPH oxidase (NOX)/reactive oxygen species (ROS) pathway. This work not only discoverers a novel pharmacological ingredient from medicinal mushroom G. lingzhi for hypertension therapy, but also provides an insight into molecular mechanism of the ACE inhibitory peptide (ACEIP) on lowering blood pressure. |
| format | Article |
| id | doaj-art-453bbb160fb0465d8ed0f0fcd874d516 |
| institution | OA Journals |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-453bbb160fb0465d8ed0f0fcd874d5162025-08-20T02:05:19ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302025-02-01142925003210.26599/FSHW.2024.9250032Discovery and mechanism of anti-hypertensive effect of a novel tripeptide (SYP) from medicinal fungus Ganoderma lingzhiQiang Wu0Feijun Luo1Jia Huang2Ping Huang3Yongnan Liu4Xiaoling Wang5Gaoqiang Liu6Hunan Provincial Key Laboratory of Forestry Biotechnology and International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha 410004, ChinaLaboratory of Molecular Nutrition, National Engineering Laboratory for Rice and Byproducts, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Provincial Key Laboratory of Forestry Biotechnology and International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Provincial Key Laboratory of Forestry Biotechnology and International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Provincial Key Laboratory of Forestry Biotechnology and International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Provincial Key Laboratory of Forestry Biotechnology and International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Provincial Key Laboratory of Forestry Biotechnology and International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha 410004, ChinaGanoderma lingzhi is a new species of the prize medicinal mushroom Ganoderma (Agaricomycetes). Using angiotensin Ⅰ-converting enzyme (ACE) as a target, a tripeptide Ser-Tyr-Pro (SYP) was discovered with preponderant ACE inhibitory activity with an 50% inhibiting concentration (IC50) value of 62.50 μg/mL attribute to the formed salt bridge and hydrogen bonds between SYP and ACE. SYP even maintained superior bioactivity after intestinal digestion, and exerted no cytotoxicity, but presented incomplete bioavailability in blood of spontaneous hypertensive rats (SHRs). Furthermore, it performed antihypertensive effect in vivo by inhibiting the influx of Ca2+ through activating endothelial NO synthase (eNOS)/NO/guanosine 3’,5’-cyclic monophosphate (cGMP) pathway, accompanied by attenuating angiotensin Ⅱ (Ang Ⅱ)/NADPH oxidase (NOX)/reactive oxygen species (ROS) pathway. This work not only discoverers a novel pharmacological ingredient from medicinal mushroom G. lingzhi for hypertension therapy, but also provides an insight into molecular mechanism of the ACE inhibitory peptide (ACEIP) on lowering blood pressure.https://www.sciopen.com/article/10.26599/FSHW.2024.9250032antihypertensive peptideganoderma lingzhigene microarraymolecular mechanism |
| spellingShingle | Qiang Wu Feijun Luo Jia Huang Ping Huang Yongnan Liu Xiaoling Wang Gaoqiang Liu Discovery and mechanism of anti-hypertensive effect of a novel tripeptide (SYP) from medicinal fungus Ganoderma lingzhi Food Science and Human Wellness antihypertensive peptide ganoderma lingzhi gene microarray molecular mechanism |
| title | Discovery and mechanism of anti-hypertensive effect of a novel tripeptide (SYP) from medicinal fungus Ganoderma lingzhi |
| title_full | Discovery and mechanism of anti-hypertensive effect of a novel tripeptide (SYP) from medicinal fungus Ganoderma lingzhi |
| title_fullStr | Discovery and mechanism of anti-hypertensive effect of a novel tripeptide (SYP) from medicinal fungus Ganoderma lingzhi |
| title_full_unstemmed | Discovery and mechanism of anti-hypertensive effect of a novel tripeptide (SYP) from medicinal fungus Ganoderma lingzhi |
| title_short | Discovery and mechanism of anti-hypertensive effect of a novel tripeptide (SYP) from medicinal fungus Ganoderma lingzhi |
| title_sort | discovery and mechanism of anti hypertensive effect of a novel tripeptide syp from medicinal fungus ganoderma lingzhi |
| topic | antihypertensive peptide ganoderma lingzhi gene microarray molecular mechanism |
| url | https://www.sciopen.com/article/10.26599/FSHW.2024.9250032 |
| work_keys_str_mv | AT qiangwu discoveryandmechanismofantihypertensiveeffectofanoveltripeptidesypfrommedicinalfungusganodermalingzhi AT feijunluo discoveryandmechanismofantihypertensiveeffectofanoveltripeptidesypfrommedicinalfungusganodermalingzhi AT jiahuang discoveryandmechanismofantihypertensiveeffectofanoveltripeptidesypfrommedicinalfungusganodermalingzhi AT pinghuang discoveryandmechanismofantihypertensiveeffectofanoveltripeptidesypfrommedicinalfungusganodermalingzhi AT yongnanliu discoveryandmechanismofantihypertensiveeffectofanoveltripeptidesypfrommedicinalfungusganodermalingzhi AT xiaolingwang discoveryandmechanismofantihypertensiveeffectofanoveltripeptidesypfrommedicinalfungusganodermalingzhi AT gaoqiangliu discoveryandmechanismofantihypertensiveeffectofanoveltripeptidesypfrommedicinalfungusganodermalingzhi |