Assessment of Stress in Dogs Under Cancer Therapy via Faecal Cortisol Metabolite Analysis: A Pilot Study

Stress reactions play an important role in animals’ ability to cope with various situations. Glucocorticoids are measured as a stress parameter, and analysis of their faecal metabolites has proven to be a good method for evaluating long term stress. We hypothesised that dogs suffering from cancer wo...

Full description

Saved in:
Bibliographic Details
Main Authors: Christina Ziegerhofer, Alexander Tichy, Miriam Kleiter, Birgitt Wolfesberger, Rupert Palme
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Animals
Subjects:
Online Access:https://www.mdpi.com/2076-2615/15/12/1809
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850156374346956800
author Christina Ziegerhofer
Alexander Tichy
Miriam Kleiter
Birgitt Wolfesberger
Rupert Palme
author_facet Christina Ziegerhofer
Alexander Tichy
Miriam Kleiter
Birgitt Wolfesberger
Rupert Palme
author_sort Christina Ziegerhofer
collection DOAJ
description Stress reactions play an important role in animals’ ability to cope with various situations. Glucocorticoids are measured as a stress parameter, and analysis of their faecal metabolites has proven to be a good method for evaluating long term stress. We hypothesised that dogs suffering from cancer would have a higher stress level during cancer therapy, which would be reflected in higher levels of faecal cortisol metabolites (FCMs). Faeces were collected from 40 dogs receiving radiation or chemotherapy before and during the treatment, and from 53 healthy dogs, who served as a control group. FCMs were extracted and quantified by a cortisol enzyme immunoassay. The results showed that the stress levels were not significantly higher in the cancer patients before therapy compared to those in the control group. Additionally, there were no significant differences in the FCM concentrations of dogs at specific time points during chemotherapy or radiotherapy. Therefore, suspected stress should not be a criterion to exclude animals from cancer treatment. Such a treatment seems ethically justifiable if it is expected to provide benefits and improvement in the quality of life for patients.
format Article
id doaj-art-4538545e08a14296ab7a10c2ffc6933a
institution OA Journals
issn 2076-2615
language English
publishDate 2025-06-01
publisher MDPI AG
record_format Article
series Animals
spelling doaj-art-4538545e08a14296ab7a10c2ffc6933a2025-08-20T02:24:34ZengMDPI AGAnimals2076-26152025-06-011512180910.3390/ani15121809Assessment of Stress in Dogs Under Cancer Therapy via Faecal Cortisol Metabolite Analysis: A Pilot StudyChristina Ziegerhofer0Alexander Tichy1Miriam Kleiter2Birgitt Wolfesberger3Rupert Palme4Department for Small Animals and Horses, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, AustriaPlatform for Bioinformatics and Biostatistics, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, AustriaDepartment for Small Animals and Horses, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, AustriaDepartment for Small Animals and Horses, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, AustriaExperimental Endocrinology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, AustriaStress reactions play an important role in animals’ ability to cope with various situations. Glucocorticoids are measured as a stress parameter, and analysis of their faecal metabolites has proven to be a good method for evaluating long term stress. We hypothesised that dogs suffering from cancer would have a higher stress level during cancer therapy, which would be reflected in higher levels of faecal cortisol metabolites (FCMs). Faeces were collected from 40 dogs receiving radiation or chemotherapy before and during the treatment, and from 53 healthy dogs, who served as a control group. FCMs were extracted and quantified by a cortisol enzyme immunoassay. The results showed that the stress levels were not significantly higher in the cancer patients before therapy compared to those in the control group. Additionally, there were no significant differences in the FCM concentrations of dogs at specific time points during chemotherapy or radiotherapy. Therefore, suspected stress should not be a criterion to exclude animals from cancer treatment. Such a treatment seems ethically justifiable if it is expected to provide benefits and improvement in the quality of life for patients.https://www.mdpi.com/2076-2615/15/12/1809stresscaninechemotherapyradiation therapyfaecesglucocorticoid metabolites
spellingShingle Christina Ziegerhofer
Alexander Tichy
Miriam Kleiter
Birgitt Wolfesberger
Rupert Palme
Assessment of Stress in Dogs Under Cancer Therapy via Faecal Cortisol Metabolite Analysis: A Pilot Study
Animals
stress
canine
chemotherapy
radiation therapy
faeces
glucocorticoid metabolites
title Assessment of Stress in Dogs Under Cancer Therapy via Faecal Cortisol Metabolite Analysis: A Pilot Study
title_full Assessment of Stress in Dogs Under Cancer Therapy via Faecal Cortisol Metabolite Analysis: A Pilot Study
title_fullStr Assessment of Stress in Dogs Under Cancer Therapy via Faecal Cortisol Metabolite Analysis: A Pilot Study
title_full_unstemmed Assessment of Stress in Dogs Under Cancer Therapy via Faecal Cortisol Metabolite Analysis: A Pilot Study
title_short Assessment of Stress in Dogs Under Cancer Therapy via Faecal Cortisol Metabolite Analysis: A Pilot Study
title_sort assessment of stress in dogs under cancer therapy via faecal cortisol metabolite analysis a pilot study
topic stress
canine
chemotherapy
radiation therapy
faeces
glucocorticoid metabolites
url https://www.mdpi.com/2076-2615/15/12/1809
work_keys_str_mv AT christinaziegerhofer assessmentofstressindogsundercancertherapyviafaecalcortisolmetaboliteanalysisapilotstudy
AT alexandertichy assessmentofstressindogsundercancertherapyviafaecalcortisolmetaboliteanalysisapilotstudy
AT miriamkleiter assessmentofstressindogsundercancertherapyviafaecalcortisolmetaboliteanalysisapilotstudy
AT birgittwolfesberger assessmentofstressindogsundercancertherapyviafaecalcortisolmetaboliteanalysisapilotstudy
AT rupertpalme assessmentofstressindogsundercancertherapyviafaecalcortisolmetaboliteanalysisapilotstudy