A revision of malbranchea-like fungi from clinical specimens in the United States of America reveals unexpected novelty
ABSTRACT The fungi of the order Onygenales can cause important human infections; however, their taxonomy and worldwide occurrence is still little known. We have studied and identified a representative number of clinical fungi belonging to that order from a reference laboratory in the USA. A total of...
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2021-09-01
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| Online Access: | https://doi.org/10.1186/s43008-021-00075-x |
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| author | Ernesto Rodríguez-Andrade José F. Cano-Lira Nathan Wiederhold Alba Pérez-Cantero Josep Guarro Alberto M. Stchigel |
| author_facet | Ernesto Rodríguez-Andrade José F. Cano-Lira Nathan Wiederhold Alba Pérez-Cantero Josep Guarro Alberto M. Stchigel |
| author_sort | Ernesto Rodríguez-Andrade |
| collection | DOAJ |
| description | ABSTRACT The fungi of the order Onygenales can cause important human infections; however, their taxonomy and worldwide occurrence is still little known. We have studied and identified a representative number of clinical fungi belonging to that order from a reference laboratory in the USA. A total of 22 strains isolated from respiratory tract (40%) and human skin and nails (27.2%) showed a malbranchea-like morphology. Six genera were phenotypically and molecularly identified, i.e. Auxarthron/Malbranchea (68.2%), Arachnomyces (9.1%), Spiromastigoides (9.1%), and Currahmyces (4.5%), and two newly proposed genera (4.5% each). Based on the results of the phylogenetic study, we synonymized Auxarthron with Malbranchea, and erected two new genera: Pseudoarthropsis and Pseudomalbranchea. New species proposed are: Arachnomyces bostrychodes, A. graciliformis, Currahmyces sparsispora, Malbranchea gymnoascoides, M. multiseptata, M. stricta, Pseudoarthropsis crassispora, Pseudomalbranchea gemmata, and Spiromastigoides geomycoides, along with a new combination for Malbranchea gypsea. The echinocandins showed the highest in vitro antifungal activity against the studied isolates, followed by terbinafine and posaconazole; in contrast, amphotericin B, fluconazole, itraconazole and 5-fluorocytosine were less active or lacked in vitro activity against these fungi. |
| format | Article |
| id | doaj-art-4537d1608def479582b4b43cf4e420b2 |
| institution | Kabale University |
| issn | 2210-6359 |
| language | English |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-4537d1608def479582b4b43cf4e420b22025-02-02T17:44:56ZengBMCIMA Fungus2210-63592021-09-0112112710.1186/s43008-021-00075-xA revision of malbranchea-like fungi from clinical specimens in the United States of America reveals unexpected noveltyErnesto Rodríguez-Andrade0José F. Cano-Lira1Nathan Wiederhold2Alba Pérez-Cantero3Josep Guarro4Alberto M. Stchigel5Mycology Unit, Medical School, Universitat Rovira i Virgili (URV)Mycology Unit, Medical School, Universitat Rovira i Virgili (URV)Fungus Testing Laboratory, University of Texas Health Science CenterMycology Unit, Medical School, Universitat Rovira i Virgili (URV)Mycology Unit, Medical School, Universitat Rovira i Virgili (URV)Mycology Unit, Medical School, Universitat Rovira i Virgili (URV)ABSTRACT The fungi of the order Onygenales can cause important human infections; however, their taxonomy and worldwide occurrence is still little known. We have studied and identified a representative number of clinical fungi belonging to that order from a reference laboratory in the USA. A total of 22 strains isolated from respiratory tract (40%) and human skin and nails (27.2%) showed a malbranchea-like morphology. Six genera were phenotypically and molecularly identified, i.e. Auxarthron/Malbranchea (68.2%), Arachnomyces (9.1%), Spiromastigoides (9.1%), and Currahmyces (4.5%), and two newly proposed genera (4.5% each). Based on the results of the phylogenetic study, we synonymized Auxarthron with Malbranchea, and erected two new genera: Pseudoarthropsis and Pseudomalbranchea. New species proposed are: Arachnomyces bostrychodes, A. graciliformis, Currahmyces sparsispora, Malbranchea gymnoascoides, M. multiseptata, M. stricta, Pseudoarthropsis crassispora, Pseudomalbranchea gemmata, and Spiromastigoides geomycoides, along with a new combination for Malbranchea gypsea. The echinocandins showed the highest in vitro antifungal activity against the studied isolates, followed by terbinafine and posaconazole; in contrast, amphotericin B, fluconazole, itraconazole and 5-fluorocytosine were less active or lacked in vitro activity against these fungi.https://doi.org/10.1186/s43008-021-00075-xAntifungalsArachnomycetalesAuxarthronClinical fungiMalbrancheaOnygenales |
| spellingShingle | Ernesto Rodríguez-Andrade José F. Cano-Lira Nathan Wiederhold Alba Pérez-Cantero Josep Guarro Alberto M. Stchigel A revision of malbranchea-like fungi from clinical specimens in the United States of America reveals unexpected novelty IMA Fungus Antifungals Arachnomycetales Auxarthron Clinical fungi Malbranchea Onygenales |
| title | A revision of malbranchea-like fungi from clinical specimens in the United States of America reveals unexpected novelty |
| title_full | A revision of malbranchea-like fungi from clinical specimens in the United States of America reveals unexpected novelty |
| title_fullStr | A revision of malbranchea-like fungi from clinical specimens in the United States of America reveals unexpected novelty |
| title_full_unstemmed | A revision of malbranchea-like fungi from clinical specimens in the United States of America reveals unexpected novelty |
| title_short | A revision of malbranchea-like fungi from clinical specimens in the United States of America reveals unexpected novelty |
| title_sort | revision of malbranchea like fungi from clinical specimens in the united states of america reveals unexpected novelty |
| topic | Antifungals Arachnomycetales Auxarthron Clinical fungi Malbranchea Onygenales |
| url | https://doi.org/10.1186/s43008-021-00075-x |
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