Method-Driven Physicochemical Profiling of <i>Aconitum pendulum</i> Bush Polysaccharides and Optimization of Extraction Protocols
<b>Background/Objectives</b>: This study aimed to characterize the physicochemical properties and antioxidant activities of polysaccharides from Aconitum pendulum Bush processed through different methods (the polysaccharide from A. pendulum (DT), the polysaccharide from A. pendulum proce...
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2025-05-01
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| author | Mingkun Meng Linlin Zhao Chunqiao Shi Yuying Song Qingya Yu Mengjia Li Xing Yang Yue Liu Tong Xu Yi Zhang |
| author_facet | Mingkun Meng Linlin Zhao Chunqiao Shi Yuying Song Qingya Yu Mengjia Li Xing Yang Yue Liu Tong Xu Yi Zhang |
| author_sort | Mingkun Meng |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: This study aimed to characterize the physicochemical properties and antioxidant activities of polysaccharides from Aconitum pendulum Bush processed through different methods (the polysaccharide from A. pendulum (DT), the polysaccharide from A. pendulum processed with zanba (Z-DT), the polysaccharide from A. pendulum processed with highland barley wine (Q-DT), and the polysaccharide from A. pendulum processed with hezi (H-DT)). Additionally, the research focused on optimizing the hot water extraction process for DT using response surface methodology (RSM) to enhance extraction efficiency and establish a scientific basis for pharmaceutical applications. <b>Methods</b>: The physicochemical properties and antioxidant activities of the four polysaccharides were systematically evaluated. RSM with a 17-run Box–Behnken design was employed to investigate the extraction process, examining three factors: extraction runs, liquid–solid ratio, and extraction time. <b>Results</b>: The physicochemical properties and antioxidant assays demonstrated that the DT exhibited significantly higher properties. The factors influencing the extraction process were ranked as extraction runs > liquid–solid ratio > extraction time. The optimal conditions for DT were a liquid–solid ratio of 25 mL/g, extraction time of 2.5 h, and four extraction runs, yielding a sugar content of 63.4%. Under these conditions, the extraction rate of DT was significantly higher than before optimization. <b>Conclusions</b>: The study demonstrated distinct structural features among the four polysaccharides, providing a scientific framework for their potential pharmaceutical applications. What’s more, the optimized hot water extraction protocol for DT was validated for high extraction rate and reproducibility. |
| format | Article |
| id | doaj-art-4531d45ea7324791b3ec5d1c48faf480 |
| institution | OA Journals |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-4531d45ea7324791b3ec5d1c48faf4802025-08-20T02:33:51ZengMDPI AGPharmaceuticals1424-82472025-05-0118576010.3390/ph18050760Method-Driven Physicochemical Profiling of <i>Aconitum pendulum</i> Bush Polysaccharides and Optimization of Extraction ProtocolsMingkun Meng0Linlin Zhao1Chunqiao Shi2Yuying Song3Qingya Yu4Mengjia Li5Xing Yang6Yue Liu7Tong Xu8Yi Zhang9State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaChengdu Institute of Chinese Herbal Medicine, Chengdu 610016, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China<b>Background/Objectives</b>: This study aimed to characterize the physicochemical properties and antioxidant activities of polysaccharides from Aconitum pendulum Bush processed through different methods (the polysaccharide from A. pendulum (DT), the polysaccharide from A. pendulum processed with zanba (Z-DT), the polysaccharide from A. pendulum processed with highland barley wine (Q-DT), and the polysaccharide from A. pendulum processed with hezi (H-DT)). Additionally, the research focused on optimizing the hot water extraction process for DT using response surface methodology (RSM) to enhance extraction efficiency and establish a scientific basis for pharmaceutical applications. <b>Methods</b>: The physicochemical properties and antioxidant activities of the four polysaccharides were systematically evaluated. RSM with a 17-run Box–Behnken design was employed to investigate the extraction process, examining three factors: extraction runs, liquid–solid ratio, and extraction time. <b>Results</b>: The physicochemical properties and antioxidant assays demonstrated that the DT exhibited significantly higher properties. The factors influencing the extraction process were ranked as extraction runs > liquid–solid ratio > extraction time. The optimal conditions for DT were a liquid–solid ratio of 25 mL/g, extraction time of 2.5 h, and four extraction runs, yielding a sugar content of 63.4%. Under these conditions, the extraction rate of DT was significantly higher than before optimization. <b>Conclusions</b>: The study demonstrated distinct structural features among the four polysaccharides, providing a scientific framework for their potential pharmaceutical applications. What’s more, the optimized hot water extraction protocol for DT was validated for high extraction rate and reproducibility.https://www.mdpi.com/1424-8247/18/5/760<i>Aconitum pendulum</i>polysaccharidesextraction |
| spellingShingle | Mingkun Meng Linlin Zhao Chunqiao Shi Yuying Song Qingya Yu Mengjia Li Xing Yang Yue Liu Tong Xu Yi Zhang Method-Driven Physicochemical Profiling of <i>Aconitum pendulum</i> Bush Polysaccharides and Optimization of Extraction Protocols Pharmaceuticals <i>Aconitum pendulum</i> polysaccharides extraction |
| title | Method-Driven Physicochemical Profiling of <i>Aconitum pendulum</i> Bush Polysaccharides and Optimization of Extraction Protocols |
| title_full | Method-Driven Physicochemical Profiling of <i>Aconitum pendulum</i> Bush Polysaccharides and Optimization of Extraction Protocols |
| title_fullStr | Method-Driven Physicochemical Profiling of <i>Aconitum pendulum</i> Bush Polysaccharides and Optimization of Extraction Protocols |
| title_full_unstemmed | Method-Driven Physicochemical Profiling of <i>Aconitum pendulum</i> Bush Polysaccharides and Optimization of Extraction Protocols |
| title_short | Method-Driven Physicochemical Profiling of <i>Aconitum pendulum</i> Bush Polysaccharides and Optimization of Extraction Protocols |
| title_sort | method driven physicochemical profiling of i aconitum pendulum i bush polysaccharides and optimization of extraction protocols |
| topic | <i>Aconitum pendulum</i> polysaccharides extraction |
| url | https://www.mdpi.com/1424-8247/18/5/760 |
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