Poly(vinyl alcohol)/Gentamicin and Poly(vinyl alcohol)/Chitosan/Gentamicin: Promising Materials for Rapid Burn Wound Healing
Scar formation and delayed wound healing pose significant challenges in treating skin injuries, especially in severe cases like burns and diabetic wounds. This study investigates the effectiveness of novel Poly(vinyl alcohol) (PVA)/Gentamicin (Gent) and PVA/Chitosan (CHI)/Gent hydrogels in promoting...
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MDPI AG
2025-05-01
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| Online Access: | https://www.mdpi.com/2310-2861/11/5/352 |
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| author | Anja Nikolić Ivan Milošević Ana Janković Bogomir Bolka Prokić Emilija Nićković Danica Marković Milena Stevanović Maja Vukašinović-Sekulić Vesna Mišković-Stanković Tijana Lužajić Božinovski |
| author_facet | Anja Nikolić Ivan Milošević Ana Janković Bogomir Bolka Prokić Emilija Nićković Danica Marković Milena Stevanović Maja Vukašinović-Sekulić Vesna Mišković-Stanković Tijana Lužajić Božinovski |
| author_sort | Anja Nikolić |
| collection | DOAJ |
| description | Scar formation and delayed wound healing pose significant challenges in treating skin injuries, especially in severe cases like burns and diabetic wounds. This study investigates the effectiveness of novel Poly(vinyl alcohol) (PVA)/Gentamicin (Gent) and PVA/Chitosan (CHI)/Gent hydrogels in promoting healing of second-degree burn wounds in a rat model. Following in vitro testing, these hydrogels were deemed non-toxic and suitable for in vivo analysis. Clinical evaluations were conducted on the 3rd, 7th, 14th, and 21st post-injury days, assessing parameters such as blistering, edema, redness, crust, bleeding, secretion, scar tissue formation, and wound contraction percentage. Histological analyses focused on re-epithelization and dermal evaluation at specific time points. Results showed that both hydrogels significantly reduced inflammation, particularly redness, by the 14th day and improved re-epithelization, with the PVA/CHI/Gent group outperforming on the 14th day and the PVA/Gent group excelling on the 21st day. Histological findings indicated increased fibroblast proliferation and collagen deposition in treated groups, suggesting enhanced dermal healing. The PVA/CHI/Gent hydrogel demonstrated notable antibacterial properties, likely due to the synergistic effects of CHI and Gent, leading to reduced inflammation and edema. Overall, both hydrogels show promise as effective wound dressings, facilitating faster healing and improved tissue recovery in burn injuries. This study supports the use of biomimetic scaffolds for enhanced wound management in clinical practices. |
| format | Article |
| id | doaj-art-451a4d8aabbc42f89fc7ecaba1d7ff4c |
| institution | DOAJ |
| issn | 2310-2861 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj-art-451a4d8aabbc42f89fc7ecaba1d7ff4c2025-08-20T03:14:36ZengMDPI AGGels2310-28612025-05-0111535210.3390/gels11050352Poly(vinyl alcohol)/Gentamicin and Poly(vinyl alcohol)/Chitosan/Gentamicin: Promising Materials for Rapid Burn Wound HealingAnja Nikolić0Ivan Milošević1Ana Janković2Bogomir Bolka Prokić3Emilija Nićković4Danica Marković5Milena Stevanović6Maja Vukašinović-Sekulić7Vesna Mišković-Stanković8Tijana Lužajić Božinovski9Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaDepartment of Histology and Embryology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaInnovation Center of the Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, SerbiaDepartment of Surgery, Orthopedy and Ophthalmology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaDepartment of Histology and Embryology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaDepartment of Histology and Embryology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaInnovation Center of the Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, SerbiaDepartment of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, SerbiaFaculty of Ecology and Environmental Protection, University Union-Nikola Tesla, Cara Dušana, 11000 Belgrade, SerbiaDepartment of Histology and Embryology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, SerbiaScar formation and delayed wound healing pose significant challenges in treating skin injuries, especially in severe cases like burns and diabetic wounds. This study investigates the effectiveness of novel Poly(vinyl alcohol) (PVA)/Gentamicin (Gent) and PVA/Chitosan (CHI)/Gent hydrogels in promoting healing of second-degree burn wounds in a rat model. Following in vitro testing, these hydrogels were deemed non-toxic and suitable for in vivo analysis. Clinical evaluations were conducted on the 3rd, 7th, 14th, and 21st post-injury days, assessing parameters such as blistering, edema, redness, crust, bleeding, secretion, scar tissue formation, and wound contraction percentage. Histological analyses focused on re-epithelization and dermal evaluation at specific time points. Results showed that both hydrogels significantly reduced inflammation, particularly redness, by the 14th day and improved re-epithelization, with the PVA/CHI/Gent group outperforming on the 14th day and the PVA/Gent group excelling on the 21st day. Histological findings indicated increased fibroblast proliferation and collagen deposition in treated groups, suggesting enhanced dermal healing. The PVA/CHI/Gent hydrogel demonstrated notable antibacterial properties, likely due to the synergistic effects of CHI and Gent, leading to reduced inflammation and edema. Overall, both hydrogels show promise as effective wound dressings, facilitating faster healing and improved tissue recovery in burn injuries. This study supports the use of biomimetic scaffolds for enhanced wound management in clinical practices.https://www.mdpi.com/2310-2861/11/5/352chitosanhealingin vitroin vivowound dressingrat |
| spellingShingle | Anja Nikolić Ivan Milošević Ana Janković Bogomir Bolka Prokić Emilija Nićković Danica Marković Milena Stevanović Maja Vukašinović-Sekulić Vesna Mišković-Stanković Tijana Lužajić Božinovski Poly(vinyl alcohol)/Gentamicin and Poly(vinyl alcohol)/Chitosan/Gentamicin: Promising Materials for Rapid Burn Wound Healing Gels chitosan healing in vitro in vivo wound dressing rat |
| title | Poly(vinyl alcohol)/Gentamicin and Poly(vinyl alcohol)/Chitosan/Gentamicin: Promising Materials for Rapid Burn Wound Healing |
| title_full | Poly(vinyl alcohol)/Gentamicin and Poly(vinyl alcohol)/Chitosan/Gentamicin: Promising Materials for Rapid Burn Wound Healing |
| title_fullStr | Poly(vinyl alcohol)/Gentamicin and Poly(vinyl alcohol)/Chitosan/Gentamicin: Promising Materials for Rapid Burn Wound Healing |
| title_full_unstemmed | Poly(vinyl alcohol)/Gentamicin and Poly(vinyl alcohol)/Chitosan/Gentamicin: Promising Materials for Rapid Burn Wound Healing |
| title_short | Poly(vinyl alcohol)/Gentamicin and Poly(vinyl alcohol)/Chitosan/Gentamicin: Promising Materials for Rapid Burn Wound Healing |
| title_sort | poly vinyl alcohol gentamicin and poly vinyl alcohol chitosan gentamicin promising materials for rapid burn wound healing |
| topic | chitosan healing in vitro in vivo wound dressing rat |
| url | https://www.mdpi.com/2310-2861/11/5/352 |
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