The Association of Matrix Metalloproteinase‐1, ‐2, ‐3, ‐7, and ‐13 Gene Polymorphisms With Peri‐Implantitis in an Iranian Population: A Case–Control Study
ABSTRACT Objectives Peri‐implantitis (PI) is the most common biological issue surrounding dental implants. According to current knowledge, the aforementioned complication is not equally distributed across different populations, and gene polymorphisms might be one contributing factor. The current stu...
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| Format: | Article |
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Wiley
2024-12-01
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| Series: | Clinical and Experimental Dental Research |
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| Online Access: | https://doi.org/10.1002/cre2.70049 |
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| author | Leila Saremi Soheil Shahbazi Mohammad Ebrahim Ghaffari Saharnaz Esmaeili Shirin Lotfipanah Reza Amid Mahdi Kadkhodazadeh |
| author_facet | Leila Saremi Soheil Shahbazi Mohammad Ebrahim Ghaffari Saharnaz Esmaeili Shirin Lotfipanah Reza Amid Mahdi Kadkhodazadeh |
| author_sort | Leila Saremi |
| collection | DOAJ |
| description | ABSTRACT Objectives Peri‐implantitis (PI) is the most common biological issue surrounding dental implants. According to current knowledge, the aforementioned complication is not equally distributed across different populations, and gene polymorphisms might be one contributing factor. The current study aimed to examine the association between gene polymorphisms of matrix metalloproteinase‐ (MMP‐) 1, ‐2, ‐3, ‐7, and ‐13 with PI in an Iranian demographic. Material and Methods The study's sample included 50 subjects suffering from PI and 89 healthy controls. From each participant, a venous blood sample of 5 cc was obtained, and DNA was extracted. Gene polymorphisms were investigated using restriction fragment length polymorphism polymerase chain reaction (RFLP‐PCR) combined with electrophoresis. Statistical analyses were done using the Pearson chi‐square test, odds ratio, and t‐test via SPSS version 28. Results The MMP‐3 (‐1171 5A/6A) and MMP‐7 (‐181 A/G) gene polymorphisms were significantly different between the patients with PI and healthy controls (PV < 0.001 and =0.025, respectively). MMP‐1 (‐1607 1G/2G), MMP‐2 (‐1306 C/T), and MMP‐13 (‐77 A/G) gene polymorphisms did not, however, differ in terms of prevalence between the two groups (PV > 0.05). Moreover, the presence of the 6 A allele in the MMP‐3 (‐1171 5A/6A) genotype resulted in a significant decrease in PI risk (PV < 0.001). Conclusions Gene polymorphisms in the genotypes of MMP‐3 (‐1171 5A/6A) and MMP‐7 (‐181 A/G) were differential when comparing PI patients and healthy controls of the studied population. |
| format | Article |
| id | doaj-art-451616da0eb542ac838da14a123eb70f |
| institution | OA Journals |
| issn | 2057-4347 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Experimental Dental Research |
| spelling | doaj-art-451616da0eb542ac838da14a123eb70f2025-08-20T02:34:53ZengWileyClinical and Experimental Dental Research2057-43472024-12-01106n/an/a10.1002/cre2.70049The Association of Matrix Metalloproteinase‐1, ‐2, ‐3, ‐7, and ‐13 Gene Polymorphisms With Peri‐Implantitis in an Iranian Population: A Case–Control StudyLeila Saremi0Soheil Shahbazi1Mohammad Ebrahim Ghaffari2Saharnaz Esmaeili3Shirin Lotfipanah4Reza Amid5Mahdi Kadkhodazadeh6Dental Research Center Shahid Beheshti University of Medical Sciences Tehran IranDentofacial Deformities Research Center, Research, Institute of Dental Sciences Shahid Beheshti University of Medical Sciences Tehran IranDepartment of Epidemiology and Biostatistics, Faculty of Health Qom university of Medical Sciences Qom IranDental Research Center Shahid Beheshti University of Medical Sciences Tehran IranDepartment of Biology Education Farhangian University Tehran IranDental Research Center Shahid Beheshti University of Medical Sciences Tehran IranDental Research Center Shahid Beheshti University of Medical Sciences Tehran IranABSTRACT Objectives Peri‐implantitis (PI) is the most common biological issue surrounding dental implants. According to current knowledge, the aforementioned complication is not equally distributed across different populations, and gene polymorphisms might be one contributing factor. The current study aimed to examine the association between gene polymorphisms of matrix metalloproteinase‐ (MMP‐) 1, ‐2, ‐3, ‐7, and ‐13 with PI in an Iranian demographic. Material and Methods The study's sample included 50 subjects suffering from PI and 89 healthy controls. From each participant, a venous blood sample of 5 cc was obtained, and DNA was extracted. Gene polymorphisms were investigated using restriction fragment length polymorphism polymerase chain reaction (RFLP‐PCR) combined with electrophoresis. Statistical analyses were done using the Pearson chi‐square test, odds ratio, and t‐test via SPSS version 28. Results The MMP‐3 (‐1171 5A/6A) and MMP‐7 (‐181 A/G) gene polymorphisms were significantly different between the patients with PI and healthy controls (PV < 0.001 and =0.025, respectively). MMP‐1 (‐1607 1G/2G), MMP‐2 (‐1306 C/T), and MMP‐13 (‐77 A/G) gene polymorphisms did not, however, differ in terms of prevalence between the two groups (PV > 0.05). Moreover, the presence of the 6 A allele in the MMP‐3 (‐1171 5A/6A) genotype resulted in a significant decrease in PI risk (PV < 0.001). Conclusions Gene polymorphisms in the genotypes of MMP‐3 (‐1171 5A/6A) and MMP‐7 (‐181 A/G) were differential when comparing PI patients and healthy controls of the studied population.https://doi.org/10.1002/cre2.70049allelesDNAgenotypematrix metalloproteinasesperi‐implantitispolymorphism |
| spellingShingle | Leila Saremi Soheil Shahbazi Mohammad Ebrahim Ghaffari Saharnaz Esmaeili Shirin Lotfipanah Reza Amid Mahdi Kadkhodazadeh The Association of Matrix Metalloproteinase‐1, ‐2, ‐3, ‐7, and ‐13 Gene Polymorphisms With Peri‐Implantitis in an Iranian Population: A Case–Control Study Clinical and Experimental Dental Research alleles DNA genotype matrix metalloproteinases peri‐implantitis polymorphism |
| title | The Association of Matrix Metalloproteinase‐1, ‐2, ‐3, ‐7, and ‐13 Gene Polymorphisms With Peri‐Implantitis in an Iranian Population: A Case–Control Study |
| title_full | The Association of Matrix Metalloproteinase‐1, ‐2, ‐3, ‐7, and ‐13 Gene Polymorphisms With Peri‐Implantitis in an Iranian Population: A Case–Control Study |
| title_fullStr | The Association of Matrix Metalloproteinase‐1, ‐2, ‐3, ‐7, and ‐13 Gene Polymorphisms With Peri‐Implantitis in an Iranian Population: A Case–Control Study |
| title_full_unstemmed | The Association of Matrix Metalloproteinase‐1, ‐2, ‐3, ‐7, and ‐13 Gene Polymorphisms With Peri‐Implantitis in an Iranian Population: A Case–Control Study |
| title_short | The Association of Matrix Metalloproteinase‐1, ‐2, ‐3, ‐7, and ‐13 Gene Polymorphisms With Peri‐Implantitis in an Iranian Population: A Case–Control Study |
| title_sort | association of matrix metalloproteinase 1 2 3 7 and 13 gene polymorphisms with peri implantitis in an iranian population a case control study |
| topic | alleles DNA genotype matrix metalloproteinases peri‐implantitis polymorphism |
| url | https://doi.org/10.1002/cre2.70049 |
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