Profiling of Metabolome in the Plasma Following a circH19 Knockdown Intervention in Diet-Induced Obese Mice
The circular RNA circH19 has been implicated in the regulation of gene expression and various biological processes, including obesity. <b>Objectives</b>: This study aimed to elucidate the metabolic changes in plasma after circH19 knockdown in a diet-induced obese (DIO) mouse model. <b...
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MDPI AG
2024-11-01
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| Series: | Metabolites |
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| author | Hanxin Zhao Dike Shi Weiwei Gui Xihua Lin Jionghuang Chen Weihua Yu |
| author_facet | Hanxin Zhao Dike Shi Weiwei Gui Xihua Lin Jionghuang Chen Weihua Yu |
| author_sort | Hanxin Zhao |
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| description | The circular RNA circH19 has been implicated in the regulation of gene expression and various biological processes, including obesity. <b>Objectives</b>: This study aimed to elucidate the metabolic changes in plasma after circH19 knockdown in a diet-induced obese (DIO) mouse model. <b>Methods:</b> Plasma samples were collected following the intervention and subjected to non-targeted metabolomics analysis using liquid chromatography–mass spectrometry (LC-MS). Metabolic profiling was performed to identify and quantify metabolites, followed by multivariate statistical analysis to discern differential metabolic signatures. <b>Results:</b> A total of 1250 features were quantified, resulting in the upregulation of 564 metabolites and the downregulation of 686 metabolites in the circH19 knockdown group compared to the control mice. Metabolic pathway analysis revealed disruptions in lipid metabolism, amino acid turnover, and energy production pathways. Notably, the intervention led to a substantial decrease in circulating lipids and alterations in the plasma amino acid profile, indicative of an impact on protein catabolism and anabolic processes. The observed shifts in lipid and amino acid metabolism suggest potential therapeutic avenues for obesity and related metabolic disorders. <b>Conclusions:</b> The circH19 knockdown in DIO mice led to significant alterations in plasma metabolites, highlighting its potential role in the regulation of obesity and metabolic disorders. |
| format | Article |
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| institution | OA Journals |
| issn | 2218-1989 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Metabolites |
| spelling | doaj-art-4503912656d04d13929bf76e1af624d42025-08-20T02:04:54ZengMDPI AGMetabolites2218-19892024-11-01141160310.3390/metabo14110603Profiling of Metabolome in the Plasma Following a circH19 Knockdown Intervention in Diet-Induced Obese MiceHanxin Zhao0Dike Shi1Weiwei Gui2Xihua Lin3Jionghuang Chen4Weihua Yu5Department of Endocrinology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, ChinaDepartment of Gastroenterology Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, ChinaDepartment of Endocrinology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, ChinaDepartment of Endocrinology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, ChinaDepartment of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, ChinaDepartment of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, ChinaThe circular RNA circH19 has been implicated in the regulation of gene expression and various biological processes, including obesity. <b>Objectives</b>: This study aimed to elucidate the metabolic changes in plasma after circH19 knockdown in a diet-induced obese (DIO) mouse model. <b>Methods:</b> Plasma samples were collected following the intervention and subjected to non-targeted metabolomics analysis using liquid chromatography–mass spectrometry (LC-MS). Metabolic profiling was performed to identify and quantify metabolites, followed by multivariate statistical analysis to discern differential metabolic signatures. <b>Results:</b> A total of 1250 features were quantified, resulting in the upregulation of 564 metabolites and the downregulation of 686 metabolites in the circH19 knockdown group compared to the control mice. Metabolic pathway analysis revealed disruptions in lipid metabolism, amino acid turnover, and energy production pathways. Notably, the intervention led to a substantial decrease in circulating lipids and alterations in the plasma amino acid profile, indicative of an impact on protein catabolism and anabolic processes. The observed shifts in lipid and amino acid metabolism suggest potential therapeutic avenues for obesity and related metabolic disorders. <b>Conclusions:</b> The circH19 knockdown in DIO mice led to significant alterations in plasma metabolites, highlighting its potential role in the regulation of obesity and metabolic disorders.https://www.mdpi.com/2218-1989/14/11/603CircRNA H19obesitymetabolitesliquid chromatography–mass spectrometry |
| spellingShingle | Hanxin Zhao Dike Shi Weiwei Gui Xihua Lin Jionghuang Chen Weihua Yu Profiling of Metabolome in the Plasma Following a circH19 Knockdown Intervention in Diet-Induced Obese Mice Metabolites CircRNA H19 obesity metabolites liquid chromatography–mass spectrometry |
| title | Profiling of Metabolome in the Plasma Following a circH19 Knockdown Intervention in Diet-Induced Obese Mice |
| title_full | Profiling of Metabolome in the Plasma Following a circH19 Knockdown Intervention in Diet-Induced Obese Mice |
| title_fullStr | Profiling of Metabolome in the Plasma Following a circH19 Knockdown Intervention in Diet-Induced Obese Mice |
| title_full_unstemmed | Profiling of Metabolome in the Plasma Following a circH19 Knockdown Intervention in Diet-Induced Obese Mice |
| title_short | Profiling of Metabolome in the Plasma Following a circH19 Knockdown Intervention in Diet-Induced Obese Mice |
| title_sort | profiling of metabolome in the plasma following a circh19 knockdown intervention in diet induced obese mice |
| topic | CircRNA H19 obesity metabolites liquid chromatography–mass spectrometry |
| url | https://www.mdpi.com/2218-1989/14/11/603 |
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