Cholinoceptor Activation Subserving the Effects of Interferon Gamma on the Contractility of Rat Ileum
Recombinant rat interferon γ stimulated the contractility of isolated rat ileum at doses of 4–12 units/ml. Muscarinic cholinoceptors were involved, as treatment of the tissue with atropine prevented the contractile response of the ileum. Furthermore, interferon γ increased the affinity of carbachol...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000645 |
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| _version_ | 1832553326123679744 |
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| author | Enri S. Borda Leonor Sterin-Borda Martin Rodriguez Maria M. E. de Bracco |
| author_facet | Enri S. Borda Leonor Sterin-Borda Martin Rodriguez Maria M. E. de Bracco |
| author_sort | Enri S. Borda |
| collection | DOAJ |
| description | Recombinant rat interferon γ stimulated the contractility of
isolated rat ileum at doses of 4–12 units/ml. Muscarinic
cholinoceptors were involved, as treatment of the tissue with
atropine prevented the contractile response of the ileum.
Furthermore, interferon γ increased the affinity of carbachol for
the cholinoceptors and did not change its maximum effect. Neurogenic
pathways were also involved since pretreatment of ileum with
hexamethonium, hemicholinium or tetrodotoxin impaired the
contractile effect of interferon γ. In contrast to the action of
exogenous carbachol, the effects of interferon γ are indirect. They
appear to involve a G protein regulating phosphoinositide turnover
and cytoskeletal structures since they could not be induced in ileum
strips that were pretreated with pertussis toxin, phospholipase C
inhibitors (2-nitro-carboxyphenyl, NN-diphenyl carbamate and
neomycin), cytochalasine B or colchicine. |
| format | Article |
| id | doaj-art-44f9066751e04563b3e553479341ec90 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1994-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-44f9066751e04563b3e553479341ec902025-02-03T05:54:17ZengWileyMediators of Inflammation0962-93511466-18611994-01-013645345810.1155/S0962935194000645Cholinoceptor Activation Subserving the Effects of Interferon Gamma on the Contractility of Rat IleumEnri S. Borda0Leonor Sterin-Borda1Martin Rodriguez2Maria M. E. de Bracco3CEFYBO-CONICET, Serrano 665, 1414 Buenos Aires, ArgentinaCEFYBO-CONICET, Serrano 665, 1414 Buenos Aires, ArgentinaCEFYBO-CONICET, Serrano 665, 1414 Buenos Aires, ArgentinaIIHEMA, Academia Nacional de Medicina, Buenos Aires, ArgentinaRecombinant rat interferon γ stimulated the contractility of isolated rat ileum at doses of 4–12 units/ml. Muscarinic cholinoceptors were involved, as treatment of the tissue with atropine prevented the contractile response of the ileum. Furthermore, interferon γ increased the affinity of carbachol for the cholinoceptors and did not change its maximum effect. Neurogenic pathways were also involved since pretreatment of ileum with hexamethonium, hemicholinium or tetrodotoxin impaired the contractile effect of interferon γ. In contrast to the action of exogenous carbachol, the effects of interferon γ are indirect. They appear to involve a G protein regulating phosphoinositide turnover and cytoskeletal structures since they could not be induced in ileum strips that were pretreated with pertussis toxin, phospholipase C inhibitors (2-nitro-carboxyphenyl, NN-diphenyl carbamate and neomycin), cytochalasine B or colchicine.http://dx.doi.org/10.1155/S0962935194000645 |
| spellingShingle | Enri S. Borda Leonor Sterin-Borda Martin Rodriguez Maria M. E. de Bracco Cholinoceptor Activation Subserving the Effects of Interferon Gamma on the Contractility of Rat Ileum Mediators of Inflammation |
| title | Cholinoceptor Activation Subserving the Effects of Interferon Gamma on the Contractility of Rat Ileum |
| title_full | Cholinoceptor Activation Subserving the Effects of Interferon Gamma on the Contractility of Rat Ileum |
| title_fullStr | Cholinoceptor Activation Subserving the Effects of Interferon Gamma on the Contractility of Rat Ileum |
| title_full_unstemmed | Cholinoceptor Activation Subserving the Effects of Interferon Gamma on the Contractility of Rat Ileum |
| title_short | Cholinoceptor Activation Subserving the Effects of Interferon Gamma on the Contractility of Rat Ileum |
| title_sort | cholinoceptor activation subserving the effects of interferon gamma on the contractility of rat ileum |
| url | http://dx.doi.org/10.1155/S0962935194000645 |
| work_keys_str_mv | AT enrisborda cholinoceptoractivationsubservingtheeffectsofinterferongammaonthecontractilityofratileum AT leonorsterinborda cholinoceptoractivationsubservingtheeffectsofinterferongammaonthecontractilityofratileum AT martinrodriguez cholinoceptoractivationsubservingtheeffectsofinterferongammaonthecontractilityofratileum AT mariamedebracco cholinoceptoractivationsubservingtheeffectsofinterferongammaonthecontractilityofratileum |