Synergistic Antibacterial Mechanism of Benzyl Isothiocyanate and Resveratrol Against <i>Staphylococcus aureus</i> Revealed by Transcriptomic Analysis and Their Application in Beef
This study aims to elucidate the synergistic antibacterial mechanism of benzyl isothiocyanate (BITC) and resveratrol (RES) on <i>Staphylococcus aureus</i> (<i>S. aureus</i>) at the transcriptional level. Compared with the individuals, the combination of BITC and RES (BITC_RES...
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MDPI AG
2025-05-01
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| author | Jianan Liu Jinle Ma Yingrui Wang Hongshun Hao Jingran Bi Hongman Hou Gongliang Zhang |
| author_facet | Jianan Liu Jinle Ma Yingrui Wang Hongshun Hao Jingran Bi Hongman Hou Gongliang Zhang |
| author_sort | Jianan Liu |
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| description | This study aims to elucidate the synergistic antibacterial mechanism of benzyl isothiocyanate (BITC) and resveratrol (RES) on <i>Staphylococcus aureus</i> (<i>S. aureus</i>) at the transcriptional level. Compared with the individuals, the combination of BITC and RES (BITC_RES) reduced <i>S. aureus</i> growth, inhibited biofilm formation, and increased cell membrane disruption. The transcriptomic results showed that the BITC_RES group presented 245 and 1150 more DEGs than the BITC group and the RES group, respectively. In addition, some other key genes in the BITC_RES group, including serine protease (<i>splA</i>, <i>splE</i>), Sae regulatory system (<i>saeR</i>, <i>saeS</i>, <i>tsaE</i>, <i>sau300</i>), accessory gene regulator protein C (<i>agrC</i>), cysteine protease (<i>sspB</i>), glutamyl endopeptidase (<i>sspA</i>), and hemolysin toxin family-related genes (<i>hly</i>, <i>lukDv</i>, <i>lukEv</i>), and the relative expression of these 12 genes was downregulated by 2.2–259.8-fold, 0.8–259.8-fold and 1.2–158.2-fold greater than those in the BITC group and the RES group, respectively. Finally, a synergistic antimicrobial effect of this combination was also observed in fresh lean beef at 4 °C and 25 °C. These findings provide information for future studies on the synergistic antimicrobial effects of BITC and RES on <i>S. aureus</i>. |
| format | Article |
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| issn | 2304-8158 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-44f446f97f924f7d8cdc3fb15eb8ef8e2025-08-20T02:24:46ZengMDPI AGFoods2304-81582025-05-01149161010.3390/foods14091610Synergistic Antibacterial Mechanism of Benzyl Isothiocyanate and Resveratrol Against <i>Staphylococcus aureus</i> Revealed by Transcriptomic Analysis and Their Application in BeefJianan Liu0Jinle Ma1Yingrui Wang2Hongshun Hao3Jingran Bi4Hongman Hou5Gongliang Zhang6School of Food Science and Technology, Dalian Polytechnic University, No. 1, Qinggongyuan, Ganjingzi District, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, No. 1, Qinggongyuan, Ganjingzi District, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, No. 1, Qinggongyuan, Ganjingzi District, Dalian 116034, ChinaDepartment of Inorganic Nonmetallic Materials Engineering, Dalian Polytechnic University, No. 1, Qinggongyuan, Ganjingzi District, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, No. 1, Qinggongyuan, Ganjingzi District, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, No. 1, Qinggongyuan, Ganjingzi District, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, No. 1, Qinggongyuan, Ganjingzi District, Dalian 116034, ChinaThis study aims to elucidate the synergistic antibacterial mechanism of benzyl isothiocyanate (BITC) and resveratrol (RES) on <i>Staphylococcus aureus</i> (<i>S. aureus</i>) at the transcriptional level. Compared with the individuals, the combination of BITC and RES (BITC_RES) reduced <i>S. aureus</i> growth, inhibited biofilm formation, and increased cell membrane disruption. The transcriptomic results showed that the BITC_RES group presented 245 and 1150 more DEGs than the BITC group and the RES group, respectively. In addition, some other key genes in the BITC_RES group, including serine protease (<i>splA</i>, <i>splE</i>), Sae regulatory system (<i>saeR</i>, <i>saeS</i>, <i>tsaE</i>, <i>sau300</i>), accessory gene regulator protein C (<i>agrC</i>), cysteine protease (<i>sspB</i>), glutamyl endopeptidase (<i>sspA</i>), and hemolysin toxin family-related genes (<i>hly</i>, <i>lukDv</i>, <i>lukEv</i>), and the relative expression of these 12 genes was downregulated by 2.2–259.8-fold, 0.8–259.8-fold and 1.2–158.2-fold greater than those in the BITC group and the RES group, respectively. Finally, a synergistic antimicrobial effect of this combination was also observed in fresh lean beef at 4 °C and 25 °C. These findings provide information for future studies on the synergistic antimicrobial effects of BITC and RES on <i>S. aureus</i>.https://www.mdpi.com/2304-8158/14/9/1610synergistic antibacterial mechanism<i>Staphylococcus aureus</i>benzyl isothiocyanateresveratroltranscriptome |
| spellingShingle | Jianan Liu Jinle Ma Yingrui Wang Hongshun Hao Jingran Bi Hongman Hou Gongliang Zhang Synergistic Antibacterial Mechanism of Benzyl Isothiocyanate and Resveratrol Against <i>Staphylococcus aureus</i> Revealed by Transcriptomic Analysis and Their Application in Beef Foods synergistic antibacterial mechanism <i>Staphylococcus aureus</i> benzyl isothiocyanate resveratrol transcriptome |
| title | Synergistic Antibacterial Mechanism of Benzyl Isothiocyanate and Resveratrol Against <i>Staphylococcus aureus</i> Revealed by Transcriptomic Analysis and Their Application in Beef |
| title_full | Synergistic Antibacterial Mechanism of Benzyl Isothiocyanate and Resveratrol Against <i>Staphylococcus aureus</i> Revealed by Transcriptomic Analysis and Their Application in Beef |
| title_fullStr | Synergistic Antibacterial Mechanism of Benzyl Isothiocyanate and Resveratrol Against <i>Staphylococcus aureus</i> Revealed by Transcriptomic Analysis and Their Application in Beef |
| title_full_unstemmed | Synergistic Antibacterial Mechanism of Benzyl Isothiocyanate and Resveratrol Against <i>Staphylococcus aureus</i> Revealed by Transcriptomic Analysis and Their Application in Beef |
| title_short | Synergistic Antibacterial Mechanism of Benzyl Isothiocyanate and Resveratrol Against <i>Staphylococcus aureus</i> Revealed by Transcriptomic Analysis and Their Application in Beef |
| title_sort | synergistic antibacterial mechanism of benzyl isothiocyanate and resveratrol against i staphylococcus aureus i revealed by transcriptomic analysis and their application in beef |
| topic | synergistic antibacterial mechanism <i>Staphylococcus aureus</i> benzyl isothiocyanate resveratrol transcriptome |
| url | https://www.mdpi.com/2304-8158/14/9/1610 |
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