VARIABLE DNA SEQUENCE OF THE pMGA GENE IN Mycoplasma gallisepticum
Specific fragments of Mycoplasma gallisepticum genomic DNA, representing different regions of pMGA1.1 and pMGA1.2 genes, were amplified by Polymerase Chain Reaction (PCR). Using sequence analysis of pMGA terminal domains, differences in nucleotide sequences among strains of M. gallisepticum were ide...
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University of Ljubljana Press (Založba Univerze v Ljubljani)
1998-12-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/17101 |
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| author | Tamara MILOŠEVIČ BERLIČ D. BENČINA P. DOVČ |
| author_facet | Tamara MILOŠEVIČ BERLIČ D. BENČINA P. DOVČ |
| author_sort | Tamara MILOŠEVIČ BERLIČ |
| collection | DOAJ |
| description | Specific fragments of Mycoplasma gallisepticum genomic DNA, representing different regions of pMGA1.1 and pMGA1.2 genes, were amplified by Polymerase Chain Reaction (PCR). Using sequence analysis of pMGA terminal domains, differences in nucleotide sequences among strains of M. gallisepticum were identified. Restriction analysis revealed polymorphism at the 5? -end of the pMGA coding region. By means of sequence analysis it was established that the most variable part of pMGA sequence was the 5? -end of the pMGA gene, the region coding amino-terminal part of pMGA protein, while the 3? -end of the gene was conserved among various M. gallisepticum strains analysed. In some strains insertions or deletions were found that indicate the diversity of the pMGA gene family and might be related to the variable expression of the pMGA gene family. Changes in nucleotide sequences of the pMGA genes were also found within isogenic lineages. Genotypic variability is also confirmed by results of the study of the gene product expression. |
| format | Article |
| id | doaj-art-44f203906e464ee8944d5fbba43f52ca |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 1998-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-44f203906e464ee8944d5fbba43f52ca2025-08-20T03:02:26ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19411998-12-0172113714410.14720/aas.1998.72.1.1710123495VARIABLE DNA SEQUENCE OF THE pMGA GENE IN Mycoplasma gallisepticumTamara MILOŠEVIČ BERLIČ0D. BENČINA1P. DOVČ2Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Domžale, SloveniaSpecific fragments of Mycoplasma gallisepticum genomic DNA, representing different regions of pMGA1.1 and pMGA1.2 genes, were amplified by Polymerase Chain Reaction (PCR). Using sequence analysis of pMGA terminal domains, differences in nucleotide sequences among strains of M. gallisepticum were identified. Restriction analysis revealed polymorphism at the 5? -end of the pMGA coding region. By means of sequence analysis it was established that the most variable part of pMGA sequence was the 5? -end of the pMGA gene, the region coding amino-terminal part of pMGA protein, while the 3? -end of the gene was conserved among various M. gallisepticum strains analysed. In some strains insertions or deletions were found that indicate the diversity of the pMGA gene family and might be related to the variable expression of the pMGA gene family. Changes in nucleotide sequences of the pMGA genes were also found within isogenic lineages. Genotypic variability is also confirmed by results of the study of the gene product expression.https://journals.uni-lj.si/aas/article/view/17101microbiologymycoplasmamycoplasmagallisepticummolecular geneticsgenespmgasequencing |
| spellingShingle | Tamara MILOŠEVIČ BERLIČ D. BENČINA P. DOVČ VARIABLE DNA SEQUENCE OF THE pMGA GENE IN Mycoplasma gallisepticum Acta Agriculturae Slovenica microbiology mycoplasma mycoplasmagallisepticum molecular genetics genes pmga sequencing |
| title | VARIABLE DNA SEQUENCE OF THE pMGA GENE IN Mycoplasma gallisepticum |
| title_full | VARIABLE DNA SEQUENCE OF THE pMGA GENE IN Mycoplasma gallisepticum |
| title_fullStr | VARIABLE DNA SEQUENCE OF THE pMGA GENE IN Mycoplasma gallisepticum |
| title_full_unstemmed | VARIABLE DNA SEQUENCE OF THE pMGA GENE IN Mycoplasma gallisepticum |
| title_short | VARIABLE DNA SEQUENCE OF THE pMGA GENE IN Mycoplasma gallisepticum |
| title_sort | variable dna sequence of the pmga gene in mycoplasma gallisepticum |
| topic | microbiology mycoplasma mycoplasmagallisepticum molecular genetics genes pmga sequencing |
| url | https://journals.uni-lj.si/aas/article/view/17101 |
| work_keys_str_mv | AT tamaramilosevicberlic variablednasequenceofthepmgageneinmycoplasmagallisepticum AT dbencina variablednasequenceofthepmgageneinmycoplasmagallisepticum AT pdovc variablednasequenceofthepmgageneinmycoplasmagallisepticum |