Identification of Challenging Dermatophyte Species Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a widely adopted technique for bacterial and yeast identification in clinical laboratories but is less frequently applied to filamentous fungi due to inconsistent performance, limitations of commercial lib...
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2025-01-01
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| author | Tsung-Fu Tsai Yun-Chen Fan Jang-Jih Lu Chun-Chih Chien Hsin-Yao Wang Pei-Lun Sun |
| author_facet | Tsung-Fu Tsai Yun-Chen Fan Jang-Jih Lu Chun-Chih Chien Hsin-Yao Wang Pei-Lun Sun |
| author_sort | Tsung-Fu Tsai |
| collection | DOAJ |
| description | Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a widely adopted technique for bacterial and yeast identification in clinical laboratories but is less frequently applied to filamentous fungi due to inconsistent performance, limitations of commercial libraries, and variability of preparation methods. This study aimed to validate the efficiency of MALDI-TOF MS-based dermatophyte identification using the Bruker Biotyper system. Focusing on species from the <i>Trichophyton</i>, <i>Nannizzia</i>, <i>Microsporum</i>, and <i>Epidermophyton</i> genera, an in-house reference library was established and evaluated with clinical isolates. The expanded library, which combined the in-house and Bruker libraries, achieved significantly higher accuracy than the Bruker library alone, correctly identifying 90.7% (107/118) of isolates at the species level compared to 16.1% (19/118) by the Bruker library. This study presents an efficient, standardized MALDI-TOF MS protocol for routine dermatophyte identification and provides a review of the current status and influencing factors in MALDI-TOF MS-based dermatophyte identification strategies. |
| format | Article |
| id | doaj-art-44eee1b64c3c45c2ad766f0cd1d174f4 |
| institution | OA Journals |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Journal of Fungi |
| spelling | doaj-art-44eee1b64c3c45c2ad766f0cd1d174f42025-08-20T02:04:01ZengMDPI AGJournal of Fungi2309-608X2025-01-0111210710.3390/jof11020107Identification of Challenging Dermatophyte Species Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass SpectrometryTsung-Fu Tsai0Yun-Chen Fan1Jang-Jih Lu2Chun-Chih Chien3Hsin-Yao Wang4Pei-Lun Sun5Department of Dermatology, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan 333, TaiwanResearch Laboratory of Medical Mycology, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan 333, TaiwanDivision of Clinical Pathology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City 231, TaiwanDepartment of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 833, TaiwanDepartment of Laboratory Medicine, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan 333, TaiwanDepartment of Dermatology, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan 333, TaiwanMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a widely adopted technique for bacterial and yeast identification in clinical laboratories but is less frequently applied to filamentous fungi due to inconsistent performance, limitations of commercial libraries, and variability of preparation methods. This study aimed to validate the efficiency of MALDI-TOF MS-based dermatophyte identification using the Bruker Biotyper system. Focusing on species from the <i>Trichophyton</i>, <i>Nannizzia</i>, <i>Microsporum</i>, and <i>Epidermophyton</i> genera, an in-house reference library was established and evaluated with clinical isolates. The expanded library, which combined the in-house and Bruker libraries, achieved significantly higher accuracy than the Bruker library alone, correctly identifying 90.7% (107/118) of isolates at the species level compared to 16.1% (19/118) by the Bruker library. This study presents an efficient, standardized MALDI-TOF MS protocol for routine dermatophyte identification and provides a review of the current status and influencing factors in MALDI-TOF MS-based dermatophyte identification strategies.https://www.mdpi.com/2309-608X/11/2/107dermatophytesfungiMALDI-TOF mass spectrometry<i>Trichophyton</i><i>Nannizzia</i><i>Microsporum</i> |
| spellingShingle | Tsung-Fu Tsai Yun-Chen Fan Jang-Jih Lu Chun-Chih Chien Hsin-Yao Wang Pei-Lun Sun Identification of Challenging Dermatophyte Species Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Journal of Fungi dermatophytes fungi MALDI-TOF mass spectrometry <i>Trichophyton</i> <i>Nannizzia</i> <i>Microsporum</i> |
| title | Identification of Challenging Dermatophyte Species Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
| title_full | Identification of Challenging Dermatophyte Species Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
| title_fullStr | Identification of Challenging Dermatophyte Species Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
| title_full_unstemmed | Identification of Challenging Dermatophyte Species Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
| title_short | Identification of Challenging Dermatophyte Species Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry |
| title_sort | identification of challenging dermatophyte species using matrix assisted laser desorption ionization time of flight mass spectrometry |
| topic | dermatophytes fungi MALDI-TOF mass spectrometry <i>Trichophyton</i> <i>Nannizzia</i> <i>Microsporum</i> |
| url | https://www.mdpi.com/2309-608X/11/2/107 |
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