<i>miR-370-3p</i> Inhibited the Proliferation of Sheep Dermal Papilla Cells by Inhibiting the Expression of <i>SMAD4</i>

<i>miR-370-3p</i> Inhibited the Proliferation of Sheep Dermal Papilla Cells by Inhibiting the Expression of <i>SMAD4</i>

The proliferation and maturation of hair follicles in follicular papilla cells are predominantly governed by miRNAs, which significantly influence the cell cycle, apoptosis, and proliferation. <i>miR-370-3p</i> has been associated with several biological processes and targets <i>SM...

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Bibliographic Details
Main Authors: Jiaqi Fu, Dan Wang, Wenqing Liu, Yu Qi, Caihong Zhang, Huansong Li, Jinshun Cai, Shuang Ji, Lichun Zhang, Fuliang Sun
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Cells
Subjects:
<i>miR-370-3p</i>
<i>SMAD4</i>
dermal papilla cells
cell phenotype
Online Access:https://www.mdpi.com/2073-4409/14/10/714
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Summary:The proliferation and maturation of hair follicles in follicular papilla cells are predominantly governed by miRNAs, which significantly influence the cell cycle, apoptosis, and proliferation. <i>miR-370-3p</i> has been associated with several biological processes and targets <i>SMAD4</i>, a crucial component in hair follicle development. Tissue expression profiling revealed significant differences in <i>miR-370-3p</i> levels between skin tissues of the two sheep breeds in January and October, as well as between tissues of the Xinji fine-wool sheep and Small-tail Han sheep. <i>SMAD4</i> exhibited significant differences in tissue-specific expression in the heart, spleen, skin, lungs, and muscles from Xinji fine-wool sheep and Small-tail Han sheep. Bioinformatics analysis and dual-luciferase reporter assays validated the regulatory interaction between <i>miR-370-3p</i> and <i>SMAD4</i>. CCK-8 experiments demonstrated that <i>miR-370-3p</i>’s targeting of <i>SMAD4</i> suppressed cell growth. Cell cycle analysis demonstrated that <i>miR-370-3p</i>’s targeting of <i>SMAD4</i> influenced the cell cycle. Annexin V-FITC/PI dual labeling demonstrated that <i>miR-370-3p</i>’s targeting of <i>SMAD4</i> promoted cell apoptosis. RT-qPCR data demonstrated that <i>miR-370-3p</i>’s targeting of <i>SMAD4</i> elevated the expression of <i>JUN</i>, <i>c-MYC</i>, and <i>TCF7L2</i> while suppressing β-catenin expression. Western blot (WB) analysis demonstrated that <i>miR-370-3p</i> targeting of <i>SMAD4</i> significantly promoted c-MYC expression while inhibiting CCND1, CCND2, and β-catenin expression. <i>miR-370-3p</i> and <i>SMAD4</i> exhibit spatiotemporal expression differences in sheep skin tissues, with widespread expression across various tissues. Furthermore, it confirmed that <i>miR-370-3p</i> targets <i>SMAD4</i> to inhibit follicular papilla cell proliferation, promote apoptosis, and influence the cell cycle.
ISSN:2073-4409

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