GJD Modulates Cardiac/Vascular Inflammation and Decreases Blood Pressure in Hypertensive Rats
Gedan Jiangya decoction (GJD) (aqueous ethanol extract), a traditional Chinese medicine formula which contain six botanical drugs (Uncaria rhynchophylla (Miq.) Miq., Salvia miltiorrhiza Bunge, Pueraria lobata (Willd.) Ohwi, Eucommia ulmoides Oliv., Prunella vulgaris L., and Achyranthes bidentata Blu...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2022/7345116 |
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| author | Shadi A. D. Mohammed Hanxing Liu Salem Baldi Pingping Chen Fang Lu Shumin Liu |
| author_facet | Shadi A. D. Mohammed Hanxing Liu Salem Baldi Pingping Chen Fang Lu Shumin Liu |
| author_sort | Shadi A. D. Mohammed |
| collection | DOAJ |
| description | Gedan Jiangya decoction (GJD) (aqueous ethanol extract), a traditional Chinese medicine formula which contain six botanical drugs (Uncaria rhynchophylla (Miq.) Miq., Salvia miltiorrhiza Bunge, Pueraria lobata (Willd.) Ohwi, Eucommia ulmoides Oliv., Prunella vulgaris L., and Achyranthes bidentata Blume) was designed to treat hypertension; however, the underlying mechanism of action is unclear. This study aimed to determine the mechanisms of action of GJD in the treatment of hypertension in spontaneously hypertensive rats (SHR). Male SHRs were randomly divided into five groups: GJD doses were low (1.36 g/kg/d), medium (2.72 g/kg/d), and high (5.44 g/kg/d), captopril (13.5 mg/kg/d), and SHR groups, with Wistar-Kyoto rats (WKY) serving as the control. Every rat was gavaged once a day. The ALC-NIBP, a noninvasive blood pressure device, measured systolic (SBP) and diastolic (DBP) blood pressures. Six weeks following treatment, all rats were anesthetized. The blood samples were obtained from the abdominal aorta and then serum isolated to assess endothelin-1 and angiotensin II, interleukin-1beta, interleukin-6, and TNF-alpha. The left ventricular and thoracic aortas were taken for HE staining, immunohistochemistry, RT-qPCR, and western blot examination. Following GJD therapy, SBP and DBP were significantly lowered, as were serum levels of endothelin-1 and angiotensin II. The thickness of the left ventricular and thoracic aorta walls reduced, as did type I collagen, type III collagen, and alpha-SMA expression in the left ventricular and aortic tissues. The GJD treatment significantly reduced serum levels of the inflammatory markers interleukin-1beta, interleukin-6, and TNF-alpha. Furthermore, interleukin-1 beta, interleukin-6, TNF-alpha, TAK1, and NF-κB/p65 levels were significantly reduced in left ventricular and aortic tissues, whereas IkB-alpha levels were significantly elevated. GJD has a dose-dependent effect on all parameters. In conclusion, GJD has been shown to lower blood pressure, improve cardiovascular remodeling, and reduce inflammation via regulating NF-κB in SHRs. |
| format | Article |
| id | doaj-art-44e1128f8fe04ada82d1b00e4a7ec536 |
| institution | Kabale University |
| issn | 1466-1861 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-44e1128f8fe04ada82d1b00e4a7ec5362025-08-20T03:36:44ZengWileyMediators of Inflammation1466-18612022-01-01202210.1155/2022/7345116GJD Modulates Cardiac/Vascular Inflammation and Decreases Blood Pressure in Hypertensive RatsShadi A. D. Mohammed0Hanxing Liu1Salem Baldi2Pingping Chen3Fang Lu4Shumin Liu5Graduate School of Heilongjiang University of Chinese MedicineGraduate School of Heilongjiang University of Chinese MedicineResearch Center of Molecular Diagnostics and SequencingInstitute of Traditional Chinese MedicineInstitute of Traditional Chinese MedicineInstitute of Traditional Chinese MedicineGedan Jiangya decoction (GJD) (aqueous ethanol extract), a traditional Chinese medicine formula which contain six botanical drugs (Uncaria rhynchophylla (Miq.) Miq., Salvia miltiorrhiza Bunge, Pueraria lobata (Willd.) Ohwi, Eucommia ulmoides Oliv., Prunella vulgaris L., and Achyranthes bidentata Blume) was designed to treat hypertension; however, the underlying mechanism of action is unclear. This study aimed to determine the mechanisms of action of GJD in the treatment of hypertension in spontaneously hypertensive rats (SHR). Male SHRs were randomly divided into five groups: GJD doses were low (1.36 g/kg/d), medium (2.72 g/kg/d), and high (5.44 g/kg/d), captopril (13.5 mg/kg/d), and SHR groups, with Wistar-Kyoto rats (WKY) serving as the control. Every rat was gavaged once a day. The ALC-NIBP, a noninvasive blood pressure device, measured systolic (SBP) and diastolic (DBP) blood pressures. Six weeks following treatment, all rats were anesthetized. The blood samples were obtained from the abdominal aorta and then serum isolated to assess endothelin-1 and angiotensin II, interleukin-1beta, interleukin-6, and TNF-alpha. The left ventricular and thoracic aortas were taken for HE staining, immunohistochemistry, RT-qPCR, and western blot examination. Following GJD therapy, SBP and DBP were significantly lowered, as were serum levels of endothelin-1 and angiotensin II. The thickness of the left ventricular and thoracic aorta walls reduced, as did type I collagen, type III collagen, and alpha-SMA expression in the left ventricular and aortic tissues. The GJD treatment significantly reduced serum levels of the inflammatory markers interleukin-1beta, interleukin-6, and TNF-alpha. Furthermore, interleukin-1 beta, interleukin-6, TNF-alpha, TAK1, and NF-κB/p65 levels were significantly reduced in left ventricular and aortic tissues, whereas IkB-alpha levels were significantly elevated. GJD has a dose-dependent effect on all parameters. In conclusion, GJD has been shown to lower blood pressure, improve cardiovascular remodeling, and reduce inflammation via regulating NF-κB in SHRs.http://dx.doi.org/10.1155/2022/7345116 |
| spellingShingle | Shadi A. D. Mohammed Hanxing Liu Salem Baldi Pingping Chen Fang Lu Shumin Liu GJD Modulates Cardiac/Vascular Inflammation and Decreases Blood Pressure in Hypertensive Rats Mediators of Inflammation |
| title | GJD Modulates Cardiac/Vascular Inflammation and Decreases Blood Pressure in Hypertensive Rats |
| title_full | GJD Modulates Cardiac/Vascular Inflammation and Decreases Blood Pressure in Hypertensive Rats |
| title_fullStr | GJD Modulates Cardiac/Vascular Inflammation and Decreases Blood Pressure in Hypertensive Rats |
| title_full_unstemmed | GJD Modulates Cardiac/Vascular Inflammation and Decreases Blood Pressure in Hypertensive Rats |
| title_short | GJD Modulates Cardiac/Vascular Inflammation and Decreases Blood Pressure in Hypertensive Rats |
| title_sort | gjd modulates cardiac vascular inflammation and decreases blood pressure in hypertensive rats |
| url | http://dx.doi.org/10.1155/2022/7345116 |
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