Plant and soil microbial composition legacies following indaziflam herbicide treatment

Land stewards in dryland ecosystems across the western U.S. face challenges to manage the exotic grass Bromus tectorum (cheatgrass), which is a poor forage, is difficult to remove, and increases risk of catastrophic fire. Managers may consider using indaziflam (Rejuvra™), a relatively new pre-emerge...

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Main Authors: Ember Sienna Bradbury, Hannah Holland-Moritz, Amy Gill, Caroline A. Havrilla
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-12-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2024.1450633/full
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author Ember Sienna Bradbury
Hannah Holland-Moritz
Amy Gill
Caroline A. Havrilla
author_facet Ember Sienna Bradbury
Hannah Holland-Moritz
Amy Gill
Caroline A. Havrilla
author_sort Ember Sienna Bradbury
collection DOAJ
description Land stewards in dryland ecosystems across the western U.S. face challenges to manage the exotic grass Bromus tectorum (cheatgrass), which is a poor forage, is difficult to remove, and increases risk of catastrophic fire. Managers may consider using indaziflam (Rejuvra™), a relatively new pre-emergent herbicide, which may reduce cheatgrass cover within drylands. However, few studies have explored the effects of indaziflam on non-target organisms. We tested how indaziflam application impacted cover and biomass of native and exotics within the plant community and composition and diversity of the soil microbiome by comparing untreated and treated arid shrubland sites in Boulder County, Colorado, USA. We found that indaziflam application decreased cheatgrass cover by as much as 80% and increased native plant cover by the same amount. Indaziflam application also was associated with increased soil nitrate (NO3−), decreased soil organic matter, and had a significant effect on the composition of the soil microbiome. Microbial community composition was significantly related to soil NO3−, soil organic matter, soil pH, and native species and cheatgrass biomass. An indicator species analysis suggested that indaziflam application shifted microbial communities. In untreated sites, ammonia-oxidizing bacteria Nitrosomonadaceae and nitrogen-digesting Opitutaceae and the fungi Articulospora proliferata were found. While in treated sites, ammonia-oxidizing archaea which are associated with intact drylands, Nitrososphaeraceae and toxin digesters and acidic-soil species Sphingomonas and Acidimicrobiia were significantly associated. Overall, these results demonstrate that indaziflam application can increase native plant recruitment, while also affecting soil properties and the soil microbiome. The findings from this study can be used to inform decision-making during dryland restoration planning process as indaziflam use may have benefits and unknown long-term consequences for the biogeochemistry and microbial ecology of the system.
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spelling doaj-art-44d87fc2e6c74557ad4bb15fbb5239ca2025-08-20T02:49:43ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-12-011510.3389/fmicb.2024.14506331450633Plant and soil microbial composition legacies following indaziflam herbicide treatmentEmber Sienna Bradbury0Hannah Holland-Moritz1Amy Gill2Caroline A. Havrilla3Department of Forestry and Rangeland Stewardship, Colorado State University, Fort Collins, CO, United StatesDepartment of Natural Resources and the Environment, University of New Hampshire, Durham, NC, United StatesDepartment of Forestry and Rangeland Stewardship, Colorado State University, Fort Collins, CO, United StatesDepartment of Forestry and Rangeland Stewardship, Colorado State University, Fort Collins, CO, United StatesLand stewards in dryland ecosystems across the western U.S. face challenges to manage the exotic grass Bromus tectorum (cheatgrass), which is a poor forage, is difficult to remove, and increases risk of catastrophic fire. Managers may consider using indaziflam (Rejuvra™), a relatively new pre-emergent herbicide, which may reduce cheatgrass cover within drylands. However, few studies have explored the effects of indaziflam on non-target organisms. We tested how indaziflam application impacted cover and biomass of native and exotics within the plant community and composition and diversity of the soil microbiome by comparing untreated and treated arid shrubland sites in Boulder County, Colorado, USA. We found that indaziflam application decreased cheatgrass cover by as much as 80% and increased native plant cover by the same amount. Indaziflam application also was associated with increased soil nitrate (NO3−), decreased soil organic matter, and had a significant effect on the composition of the soil microbiome. Microbial community composition was significantly related to soil NO3−, soil organic matter, soil pH, and native species and cheatgrass biomass. An indicator species analysis suggested that indaziflam application shifted microbial communities. In untreated sites, ammonia-oxidizing bacteria Nitrosomonadaceae and nitrogen-digesting Opitutaceae and the fungi Articulospora proliferata were found. While in treated sites, ammonia-oxidizing archaea which are associated with intact drylands, Nitrososphaeraceae and toxin digesters and acidic-soil species Sphingomonas and Acidimicrobiia were significantly associated. Overall, these results demonstrate that indaziflam application can increase native plant recruitment, while also affecting soil properties and the soil microbiome. The findings from this study can be used to inform decision-making during dryland restoration planning process as indaziflam use may have benefits and unknown long-term consequences for the biogeochemistry and microbial ecology of the system.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1450633/fullindaziflamcheatgrassrestoration ecologysoil microbial communityIntermountain West (US)
spellingShingle Ember Sienna Bradbury
Hannah Holland-Moritz
Amy Gill
Caroline A. Havrilla
Plant and soil microbial composition legacies following indaziflam herbicide treatment
Frontiers in Microbiology
indaziflam
cheatgrass
restoration ecology
soil microbial community
Intermountain West (US)
title Plant and soil microbial composition legacies following indaziflam herbicide treatment
title_full Plant and soil microbial composition legacies following indaziflam herbicide treatment
title_fullStr Plant and soil microbial composition legacies following indaziflam herbicide treatment
title_full_unstemmed Plant and soil microbial composition legacies following indaziflam herbicide treatment
title_short Plant and soil microbial composition legacies following indaziflam herbicide treatment
title_sort plant and soil microbial composition legacies following indaziflam herbicide treatment
topic indaziflam
cheatgrass
restoration ecology
soil microbial community
Intermountain West (US)
url https://www.frontiersin.org/articles/10.3389/fmicb.2024.1450633/full
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