Analytical Method Development for Sodium Valproate through Chemical Derivatization
Background. Sodium valproate has anticonvulsant activity and is structurally different to conventional antiepileptic drugs. The problem with valproic acid is the lack of a chromophore, which means that gas chromatography is the sole assay methodology. The introduction of benzoyl and phenyl groups to...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/5672183 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832549928190083072 |
|---|---|
| author | Murad Abualhasan Nihaya Wasif Odeh Ghadeer Naser Younis Oyoun Fadel Zeidan |
| author_facet | Murad Abualhasan Nihaya Wasif Odeh Ghadeer Naser Younis Oyoun Fadel Zeidan |
| author_sort | Murad Abualhasan |
| collection | DOAJ |
| description | Background. Sodium valproate has anticonvulsant activity and is structurally different to conventional antiepileptic drugs. The problem with valproic acid is the lack of a chromophore, which means that gas chromatography is the sole assay methodology. The introduction of benzoyl and phenyl groups to the molecule is a useful derivatisation, which enables the creation of detectable chromophores for HPLC analysis for pharmaceutical dosages as well as biological systems. Methodology. Sodium valproate was derivatised by the addition of a chromophore to its structure by introducing a methyl benzoyl or a phenyl group. Trichlorophenol and 2-hydroxyacetophenone were used to introduce phenyl and benzoyl groups to valproic acid, respectively. The reaction used was estrification reaction using coupling agents. An analytical method was then developed and validated using reverse-phase HPLC. The method was validated for parameters like linearity, range, accuracy precision, and robustness. Results. The developed method was easy and feasible and can be applied to both routine analysis and bioanalysis. The method was very sensitive and could quantify valproic acid at a very low concentration of 0.75 × 10−5 mg/ml. The developed method was found to be linear (R2 = 0.997), accurate, precise, and robust. Conclusion. The proposed chemical derivatisation and the developed analytical method are novel. The developed analytical procedure is the first of its kind; it is easy and feasible and can be used to quantify and detect sodium valproate at very low concentrations compared to other available methods in the literature. |
| format | Article |
| id | doaj-art-44cfa07de42442eaa5adc710060b87e9 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-44cfa07de42442eaa5adc710060b87e92025-02-03T06:08:07ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792020-01-01202010.1155/2020/56721835672183Analytical Method Development for Sodium Valproate through Chemical DerivatizationMurad Abualhasan0Nihaya Wasif Odeh1Ghadeer Naser Younis2Oyoun Fadel Zeidan3Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, P.O. Box 7, Nablus, State of PalestineDepartment of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, P.O. Box 7, Nablus, State of PalestineDepartment of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, P.O. Box 7, Nablus, State of PalestineDepartment of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, P.O. Box 7, Nablus, State of PalestineBackground. Sodium valproate has anticonvulsant activity and is structurally different to conventional antiepileptic drugs. The problem with valproic acid is the lack of a chromophore, which means that gas chromatography is the sole assay methodology. The introduction of benzoyl and phenyl groups to the molecule is a useful derivatisation, which enables the creation of detectable chromophores for HPLC analysis for pharmaceutical dosages as well as biological systems. Methodology. Sodium valproate was derivatised by the addition of a chromophore to its structure by introducing a methyl benzoyl or a phenyl group. Trichlorophenol and 2-hydroxyacetophenone were used to introduce phenyl and benzoyl groups to valproic acid, respectively. The reaction used was estrification reaction using coupling agents. An analytical method was then developed and validated using reverse-phase HPLC. The method was validated for parameters like linearity, range, accuracy precision, and robustness. Results. The developed method was easy and feasible and can be applied to both routine analysis and bioanalysis. The method was very sensitive and could quantify valproic acid at a very low concentration of 0.75 × 10−5 mg/ml. The developed method was found to be linear (R2 = 0.997), accurate, precise, and robust. Conclusion. The proposed chemical derivatisation and the developed analytical method are novel. The developed analytical procedure is the first of its kind; it is easy and feasible and can be used to quantify and detect sodium valproate at very low concentrations compared to other available methods in the literature.http://dx.doi.org/10.1155/2020/5672183 |
| spellingShingle | Murad Abualhasan Nihaya Wasif Odeh Ghadeer Naser Younis Oyoun Fadel Zeidan Analytical Method Development for Sodium Valproate through Chemical Derivatization International Journal of Analytical Chemistry |
| title | Analytical Method Development for Sodium Valproate through Chemical Derivatization |
| title_full | Analytical Method Development for Sodium Valproate through Chemical Derivatization |
| title_fullStr | Analytical Method Development for Sodium Valproate through Chemical Derivatization |
| title_full_unstemmed | Analytical Method Development for Sodium Valproate through Chemical Derivatization |
| title_short | Analytical Method Development for Sodium Valproate through Chemical Derivatization |
| title_sort | analytical method development for sodium valproate through chemical derivatization |
| url | http://dx.doi.org/10.1155/2020/5672183 |
| work_keys_str_mv | AT muradabualhasan analyticalmethoddevelopmentforsodiumvalproatethroughchemicalderivatization AT nihayawasifodeh analyticalmethoddevelopmentforsodiumvalproatethroughchemicalderivatization AT ghadeernaseryounis analyticalmethoddevelopmentforsodiumvalproatethroughchemicalderivatization AT oyounfadelzeidan analyticalmethoddevelopmentforsodiumvalproatethroughchemicalderivatization |