INTERNALIZATION OF EXOGENOUS DNA INTO INTERIOR COMPARTMENTS OF MURINE BONE MARROW CELLS
Injections ofexogenous DNAin combination witha cytostatic agent cyclophosphamide (CP) cause illness anddeathofexperimental mice. This phenomenon is referredto this as delayeddeath. It has been foundthat upon intraperitoneal injections fragments ofexogenous DNAreachthe bone marrowniche andenter the n...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2014-12-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/56 |
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| author | E. V. Dolgova V. P. Nicolin N. A. Popova A. S. Proskurina K. E. Orishchenko E. A. Alyamkina Y. R. Efremov E. R. Chernykh A. A. Ostanin E. M. Malkova O. S. Taranov V. A. Rogachev S. V. Sidorov S. S. Bogachev M. A. Shurdov |
| author_facet | E. V. Dolgova V. P. Nicolin N. A. Popova A. S. Proskurina K. E. Orishchenko E. A. Alyamkina Y. R. Efremov E. R. Chernykh A. A. Ostanin E. M. Malkova O. S. Taranov V. A. Rogachev S. V. Sidorov S. S. Bogachev M. A. Shurdov |
| author_sort | E. V. Dolgova |
| collection | DOAJ |
| description | Injections ofexogenous DNAin combination witha cytostatic agent cyclophosphamide (CP) cause illness anddeathofexperimental mice. This phenomenon is referredto this as delayeddeath. It has been foundthat upon intraperitoneal injections fragments ofexogenous DNAreachthe bone marrowniche andenter the nuclei ofbone marrowcells (BMCs). Also, fragments ofexogenous DNAare capturedfrom culture medium byBMCs generatedex vivo. Upon suchtreatment, the fragments are incorporatedinto cells in a non-degradedform. We estimate that under suchconditions upto 1800 kb ofexogenous DNAcan be present in BMCs as fragments. After ex vivo incubation withBMCs, fragments ofexogenous DNAinternalizedin cells ofbothintact andCP pre-treatedmice become circularized. It has been shown that fragments ofexogenous DNAcan form high-molecular weight structures in vivo. Moreover, fragments localizedin BMCnuclei are suggestedto integrate into chromosome(s) ofrecipient mouse genomes when treatedwithCP andexogenous DNA. |
| format | Article |
| id | doaj-art-44ca9a79f2b04c9ebee690df159623c6 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2014-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-44ca9a79f2b04c9ebee690df159623c62025-02-01T09:57:58ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592014-12-0116239741440INTERNALIZATION OF EXOGENOUS DNA INTO INTERIOR COMPARTMENTS OF MURINE BONE MARROW CELLSE. V. Dolgova0V. P. Nicolin1N. A. Popova2A. S. Proskurina3K. E. Orishchenko4E. A. Alyamkina5Y. R. Efremov6E. R. Chernykh7A. A. Ostanin8E. M. Malkova9O. S. Taranov10V. A. Rogachev11S. V. Sidorov12S. S. Bogachev13M. A. Shurdov14Institute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk, RussiaInstitute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk, RussiaState Research Center of Virology and Biotechnology Vector, Novosibirsk, RussiaState Research Center of Virology and Biotechnology Vector, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaCity Clinical Hospital No. 1, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaООО Panagen, Novosibirsk, RussiaInjections ofexogenous DNAin combination witha cytostatic agent cyclophosphamide (CP) cause illness anddeathofexperimental mice. This phenomenon is referredto this as delayeddeath. It has been foundthat upon intraperitoneal injections fragments ofexogenous DNAreachthe bone marrowniche andenter the nuclei ofbone marrowcells (BMCs). Also, fragments ofexogenous DNAare capturedfrom culture medium byBMCs generatedex vivo. Upon suchtreatment, the fragments are incorporatedinto cells in a non-degradedform. We estimate that under suchconditions upto 1800 kb ofexogenous DNAcan be present in BMCs as fragments. After ex vivo incubation withBMCs, fragments ofexogenous DNAinternalizedin cells ofbothintact andCP pre-treatedmice become circularized. It has been shown that fragments ofexogenous DNAcan form high-molecular weight structures in vivo. Moreover, fragments localizedin BMCnuclei are suggestedto integrate into chromosome(s) ofrecipient mouse genomes when treatedwithCP andexogenous DNA.https://vavilov.elpub.ru/jour/article/view/56bone marrow cellsfragments of exogenous dnaepisomeconcatemerization |
| spellingShingle | E. V. Dolgova V. P. Nicolin N. A. Popova A. S. Proskurina K. E. Orishchenko E. A. Alyamkina Y. R. Efremov E. R. Chernykh A. A. Ostanin E. M. Malkova O. S. Taranov V. A. Rogachev S. V. Sidorov S. S. Bogachev M. A. Shurdov INTERNALIZATION OF EXOGENOUS DNA INTO INTERIOR COMPARTMENTS OF MURINE BONE MARROW CELLS Вавиловский журнал генетики и селекции bone marrow cells fragments of exogenous dna episome concatemerization |
| title | INTERNALIZATION OF EXOGENOUS DNA INTO INTERIOR COMPARTMENTS OF MURINE BONE MARROW CELLS |
| title_full | INTERNALIZATION OF EXOGENOUS DNA INTO INTERIOR COMPARTMENTS OF MURINE BONE MARROW CELLS |
| title_fullStr | INTERNALIZATION OF EXOGENOUS DNA INTO INTERIOR COMPARTMENTS OF MURINE BONE MARROW CELLS |
| title_full_unstemmed | INTERNALIZATION OF EXOGENOUS DNA INTO INTERIOR COMPARTMENTS OF MURINE BONE MARROW CELLS |
| title_short | INTERNALIZATION OF EXOGENOUS DNA INTO INTERIOR COMPARTMENTS OF MURINE BONE MARROW CELLS |
| title_sort | internalization of exogenous dna into interior compartments of murine bone marrow cells |
| topic | bone marrow cells fragments of exogenous dna episome concatemerization |
| url | https://vavilov.elpub.ru/jour/article/view/56 |
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