Development of haploid winter wheat by the method of distant hybridization with maize

In order to improve the breeding process and expand the genetic diversity of winter wheat, haploid lines can serve as new initial material. One of the methods for developing haploids in wheat plants is distant hybridization when crossing with maize plants and subsequent selective elimination of chro...

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Main Authors: N. V. Kalinina, V. Yu. Dontsova, N. G. Chertkova, D. M. Marchenko
Format: Article
Language:Russian
Published: Federal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”" 2024-12-01
Series:Зерновое хозяйство России
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Online Access:https://www.zhros.online/jour/article/view/2876
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author N. V. Kalinina
V. Yu. Dontsova
N. G. Chertkova
D. M. Marchenko
author_facet N. V. Kalinina
V. Yu. Dontsova
N. G. Chertkova
D. M. Marchenko
author_sort N. V. Kalinina
collection DOAJ
description In order to improve the breeding process and expand the genetic diversity of winter wheat, haploid lines can serve as new initial material. One of the methods for developing haploids in wheat plants is distant hybridization when crossing with maize plants and subsequent selective elimination of chromosomes. Postgamic incompatibility of parent plants can be overcome by growing embryos in vitro. The purpose of the current study was to develop haploid winter common wheat plants using the method of distant hybridization when crossing with maize and selective elimination of chromosomes. The current paper has presented the hybridization results of winter wheat varieties and hybrids ([(‘Odesskaya 200’ x ‘Agrofak 100’) x ‘Razdolie’], ‘1608/21’, ‘1638/19’, ‘Stanichnaya’, ‘221/20’, ‘Volnitsa’, ‘Knyaginya Olga’, ‘Bezostaya 100’, ‘Alekseich’) and haploproducer lines of maize (SM7, Kr 935/86, Kr 651) using the embryoculture method in vitro. As a result, there was established that the mean setting rate of hybrid grains was 64 %. The highest percentage of grains with embryos was in the combinations ‘Stanichnaya x SM7’ (30 %), ‘Bezostaya 100 x SM7’ (33 %) and ‘Volnitsa x SM7’ (36 %). The maximum number of haploid sprouts was formed in the hybrid combinations ‘Stanichnaya x SM7’ (8 pcs.), ‘Bezostaya 100 x SM7’ and ‘Volnitsa x SM7’ (7 and 7 pcs.). There were developed 37 haploid plants from 13 cross combinations. The survival rate of plants after putting into soil was 62 %. It is being planned to evaluate the developed breeding material.
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issn 2079-8725
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publishDate 2024-12-01
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spelling doaj-art-44c78d204be34fbda9cb0780eabee2ca2025-08-20T03:19:03ZrusFederal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”"Зерновое хозяйство России2079-87252079-87332024-12-01166748010.31367/2079-8725-2024-95-6-74-80999Development of haploid winter wheat by the method of distant hybridization with maizeN. V. Kalinina0V. Yu. Dontsova1N. G. Chertkova2D. M. Marchenko3FSBSI Agricultural Research Center “Donskoy”FSBSI Agricultural Research Center “Donskoy”FSBSI Agricultural Research Center “Donskoy”FSBSI Agricultural Research Center “Donskoy”In order to improve the breeding process and expand the genetic diversity of winter wheat, haploid lines can serve as new initial material. One of the methods for developing haploids in wheat plants is distant hybridization when crossing with maize plants and subsequent selective elimination of chromosomes. Postgamic incompatibility of parent plants can be overcome by growing embryos in vitro. The purpose of the current study was to develop haploid winter common wheat plants using the method of distant hybridization when crossing with maize and selective elimination of chromosomes. The current paper has presented the hybridization results of winter wheat varieties and hybrids ([(‘Odesskaya 200’ x ‘Agrofak 100’) x ‘Razdolie’], ‘1608/21’, ‘1638/19’, ‘Stanichnaya’, ‘221/20’, ‘Volnitsa’, ‘Knyaginya Olga’, ‘Bezostaya 100’, ‘Alekseich’) and haploproducer lines of maize (SM7, Kr 935/86, Kr 651) using the embryoculture method in vitro. As a result, there was established that the mean setting rate of hybrid grains was 64 %. The highest percentage of grains with embryos was in the combinations ‘Stanichnaya x SM7’ (30 %), ‘Bezostaya 100 x SM7’ (33 %) and ‘Volnitsa x SM7’ (36 %). The maximum number of haploid sprouts was formed in the hybrid combinations ‘Stanichnaya x SM7’ (8 pcs.), ‘Bezostaya 100 x SM7’ and ‘Volnitsa x SM7’ (7 and 7 pcs.). There were developed 37 haploid plants from 13 cross combinations. The survival rate of plants after putting into soil was 62 %. It is being planned to evaluate the developed breeding material.https://www.zhros.online/jour/article/view/2876winter wheatmaizehaploidsdistant hybridizationchromosome elimination
spellingShingle N. V. Kalinina
V. Yu. Dontsova
N. G. Chertkova
D. M. Marchenko
Development of haploid winter wheat by the method of distant hybridization with maize
Зерновое хозяйство России
winter wheat
maize
haploids
distant hybridization
chromosome elimination
title Development of haploid winter wheat by the method of distant hybridization with maize
title_full Development of haploid winter wheat by the method of distant hybridization with maize
title_fullStr Development of haploid winter wheat by the method of distant hybridization with maize
title_full_unstemmed Development of haploid winter wheat by the method of distant hybridization with maize
title_short Development of haploid winter wheat by the method of distant hybridization with maize
title_sort development of haploid winter wheat by the method of distant hybridization with maize
topic winter wheat
maize
haploids
distant hybridization
chromosome elimination
url https://www.zhros.online/jour/article/view/2876
work_keys_str_mv AT nvkalinina developmentofhaploidwinterwheatbythemethodofdistanthybridizationwithmaize
AT vyudontsova developmentofhaploidwinterwheatbythemethodofdistanthybridizationwithmaize
AT ngchertkova developmentofhaploidwinterwheatbythemethodofdistanthybridizationwithmaize
AT dmmarchenko developmentofhaploidwinterwheatbythemethodofdistanthybridizationwithmaize