Application of protein A immunoadsorption in thyroid-associated ophthalmopathy (TAO): study protocol for a non-randomised controlled clinical trial

Introduction Thyroid-associated antibodies are crucial in the occurrence and development of thyroid-associated ophthalmopathy (TAO). Protein A immunoadsorption is a more specific and plasma-independent antibody removal technology, which has been used in a variety of autoimmune diseases, such as syst...

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Main Authors: Feng Zhang, Wei Xiong, Haiyan Chen, Shiyao Lu, Shenghua Liu
Format: Article
Language:English
Published: BMJ Publishing Group 2025-07-01
Series:BMJ Open
Online Access:https://bmjopen.bmj.com/content/15/7/e099519.full
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author Feng Zhang
Wei Xiong
Haiyan Chen
Shiyao Lu
Shenghua Liu
author_facet Feng Zhang
Wei Xiong
Haiyan Chen
Shiyao Lu
Shenghua Liu
author_sort Feng Zhang
collection DOAJ
description Introduction Thyroid-associated antibodies are crucial in the occurrence and development of thyroid-associated ophthalmopathy (TAO). Protein A immunoadsorption is a more specific and plasma-independent antibody removal technology, which has been used in a variety of autoimmune diseases, such as systemic lupus erythematosus and antineutrophil cytoplasmic antibodies associated vasculitis (ANCA-associated vasculitis). This study protocol aims to verify the efficacy and safety of the application of protein A immunoadsorption in TAO to provide a new method for the treatment of this pressing disease.Methods and analysis This clinical trial is a single-centre, prospective, non-randomised controlled trial in China. 174 screened patients with moderate to severe active TAO will be divided into three groups: protein A immunoadsorption treatment group, methylprednisolone hormone pulse group and tocilizumab injection group, with 58 patients in each group. They will undergo 1-year treatment and follow-up. The primary outcomes include: (1) improvement of eyelid retraction greater than 2 mm; (2) a reduction of the Clinical Activity Score (CAS) by ≥1 point; (3) the exophthalmos retreats to ≥2 mm. The secondary outcomes include: (1) Improvement of Graves’ Ophthalmopathy Quality of Life (GO-QOL) questionnaire score after 6 months of treatment; (2) reduction of thyroid-associated antibody titre. Treatment is considered effective if two or more primary outcomes are met or if one primary outcome and two secondary outcomes are met. Otherwise, the treatment is ineffective. The evaluation of the results is based on the most severely affected eye, and the efficacy is evaluated by the outcome of this eye at the end point.Ethics and dissemination Ethical approval has been obtained from the Institutional Review Board (IRB) of Third Xiangya Hospital, Central South University. Findings will be reported to (1) participating patients; (2) funding bodies, institutes and the hospital; (3) presented at local, national and international conferences and (4) disseminated by peer-review publications.Trial registration number ChiCTR 2500095461. Registered on 7 January 2025; https://www.chictr.org.cn/.
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spelling doaj-art-44bfab2cca504c3386cc162d43a5ff6a2025-08-20T03:25:08ZengBMJ Publishing GroupBMJ Open2044-60552025-07-0115710.1136/bmjopen-2025-099519Application of protein A immunoadsorption in thyroid-associated ophthalmopathy (TAO): study protocol for a non-randomised controlled clinical trialFeng Zhang0Wei Xiong1Haiyan Chen2Shiyao Lu3Shenghua Liu4Department of Ophthalmology, Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaDepartment of Ophthalmology, Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaDepartment of Ophthalmology, GongRen Hospital of WuZhou, Wuzhou, Guangxi Zhuang Autonomous Region, ChinaDepartment of Ophthalmology, Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaDepartment of Ophthalmology, Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaIntroduction Thyroid-associated antibodies are crucial in the occurrence and development of thyroid-associated ophthalmopathy (TAO). Protein A immunoadsorption is a more specific and plasma-independent antibody removal technology, which has been used in a variety of autoimmune diseases, such as systemic lupus erythematosus and antineutrophil cytoplasmic antibodies associated vasculitis (ANCA-associated vasculitis). This study protocol aims to verify the efficacy and safety of the application of protein A immunoadsorption in TAO to provide a new method for the treatment of this pressing disease.Methods and analysis This clinical trial is a single-centre, prospective, non-randomised controlled trial in China. 174 screened patients with moderate to severe active TAO will be divided into three groups: protein A immunoadsorption treatment group, methylprednisolone hormone pulse group and tocilizumab injection group, with 58 patients in each group. They will undergo 1-year treatment and follow-up. The primary outcomes include: (1) improvement of eyelid retraction greater than 2 mm; (2) a reduction of the Clinical Activity Score (CAS) by ≥1 point; (3) the exophthalmos retreats to ≥2 mm. The secondary outcomes include: (1) Improvement of Graves’ Ophthalmopathy Quality of Life (GO-QOL) questionnaire score after 6 months of treatment; (2) reduction of thyroid-associated antibody titre. Treatment is considered effective if two or more primary outcomes are met or if one primary outcome and two secondary outcomes are met. Otherwise, the treatment is ineffective. The evaluation of the results is based on the most severely affected eye, and the efficacy is evaluated by the outcome of this eye at the end point.Ethics and dissemination Ethical approval has been obtained from the Institutional Review Board (IRB) of Third Xiangya Hospital, Central South University. Findings will be reported to (1) participating patients; (2) funding bodies, institutes and the hospital; (3) presented at local, national and international conferences and (4) disseminated by peer-review publications.Trial registration number ChiCTR 2500095461. Registered on 7 January 2025; https://www.chictr.org.cn/.https://bmjopen.bmj.com/content/15/7/e099519.full
spellingShingle Feng Zhang
Wei Xiong
Haiyan Chen
Shiyao Lu
Shenghua Liu
Application of protein A immunoadsorption in thyroid-associated ophthalmopathy (TAO): study protocol for a non-randomised controlled clinical trial
BMJ Open
title Application of protein A immunoadsorption in thyroid-associated ophthalmopathy (TAO): study protocol for a non-randomised controlled clinical trial
title_full Application of protein A immunoadsorption in thyroid-associated ophthalmopathy (TAO): study protocol for a non-randomised controlled clinical trial
title_fullStr Application of protein A immunoadsorption in thyroid-associated ophthalmopathy (TAO): study protocol for a non-randomised controlled clinical trial
title_full_unstemmed Application of protein A immunoadsorption in thyroid-associated ophthalmopathy (TAO): study protocol for a non-randomised controlled clinical trial
title_short Application of protein A immunoadsorption in thyroid-associated ophthalmopathy (TAO): study protocol for a non-randomised controlled clinical trial
title_sort application of protein a immunoadsorption in thyroid associated ophthalmopathy tao study protocol for a non randomised controlled clinical trial
url https://bmjopen.bmj.com/content/15/7/e099519.full
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