Genomic Characterization of Selected <i>Escherichia coli</i> Strains from Catfish (<i>Clarias gariepinus</i>) in Nigeria
According to a report by the World Health Organization (WHO), each year, over 550 million individuals worldwide suffer from and 230,000 die from diarrheal illnesses, which accounts for more than half of the global foodborne disease burden. Among them, children face a heightened vulnerability, with a...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-07-01
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| Series: | Applied Microbiology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-8007/4/3/77 |
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| Summary: | According to a report by the World Health Organization (WHO), each year, over 550 million individuals worldwide suffer from and 230,000 die from diarrheal illnesses, which accounts for more than half of the global foodborne disease burden. Among them, children face a heightened vulnerability, with approximately 220 million falling ill and 96,000 succumbing to these diseases annually. This work aimed to study the genomic characterization of selected <i>E. coli</i> strains from catfish (<i>Clarias</i> (<i>C.</i>) <i>gariepinus</i>) caught from the Onitsha North axis of the River Niger in Anambra state, Nigeria. A total of 50 fish were randomly purchased from different fishermen over a period of four months. Samples that comprised six different organs (skin, flesh, gills, gonads, guts, and liver) were screened for <i>E. coli</i> strains using cultural and biochemical methods. Multilocus sequence typing (MLST) and core genome (cg)MLST were performed using Ridom SeqSphere+ software. The aerobic plate count (APC) and coliform count ranged from 0.5 × 10<sup>4</sup> to 3.7 × 10<sup>4</sup> cfu/g and 0 to 3.0 × 10<sup>4</sup> cfu/g, respectively. Whole-genome sequencing (WGS) confirmed the presence of <i>E. coli</i> and <i>Klebsiella quasipneumoniae</i> isolates in our samples. We could identify only two serotypes (O102:H7 and O40:H4) of <i>E. coli</i>. Antimicrobial resistance genes (ARGs) and point mutations that conferred antibiotic resistance were extracted from the genome assemblies. Good hygiene is recommended to avoid the cross-contamination of raw <i>C. gariepinus</i> with ready-to-eat food. |
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| ISSN: | 2673-8007 |