In situ soft tissue regeneration using periosteal distraction: A preliminary study in the rat calvarial model

Aim: In this study, we aimed to evaluate soft tissue generated by periosteal distraction. Background: Management of soft tissue defects represents a challenge in dentistry. Previous periosteal distraction studies documented partial fill of the distraction space with newly-generated bone and fibrous...

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Main Author: Osama Zakaria
Format: Article
Language:English
Published: Springer 2021-11-01
Series:Saudi Dental Journal
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Online Access:http://www.sciencedirect.com/science/article/pii/S1013905220307628
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author Osama Zakaria
author_facet Osama Zakaria
author_sort Osama Zakaria
collection DOAJ
description Aim: In this study, we aimed to evaluate soft tissue generated by periosteal distraction. Background: Management of soft tissue defects represents a challenge in dentistry. Previous periosteal distraction studies documented partial fill of the distraction space with newly-generated bone and fibrous connective tissue. Material and methods: Titanium meshes were inserted in subperiosteal tunnels in the calvaria of 20 rats through coronal incision. The devices were immediately activated after insertion by elevation of one side at 1 mm/day for 3 days. Rats were then divided into two groups (n = 10). Animals were sacrificed after 2 weeks (Group 1) and after 4 weeks (Group 2). Distraction sites specimens were embedded in paraffin and analyzed histologically and histomorphometrically. Results: In both groups, new periosteum was regenerated and covered the original bone surface in the distraction site. Distraction spaces showed a predomination of hyper-vascularized connective tissue and little new bone formation near to the stable end of the device. The 4-week findings showed more organized collagen fibers with less vascularity compared to the 2-week findings. Conclusion: The periosteal distraction technique can effectively regenerate connective tissue. It may open a new modality in the guided tissue regeneration for soft tissue augmentation.
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spelling doaj-art-44b1cc865ffe4ae886fc9ba68e9e9af12025-08-20T02:15:37ZengSpringerSaudi Dental Journal1013-90522021-11-0133758759410.1016/j.sdentj.2020.06.001In situ soft tissue regeneration using periosteal distraction: A preliminary study in the rat calvarial modelOsama Zakaria0Department of Biomedical Dental Sciences, Oral and Maxillofacial Division, College of Dentistry, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, 31441 Dammam, Saudi ArabiaAim: In this study, we aimed to evaluate soft tissue generated by periosteal distraction. Background: Management of soft tissue defects represents a challenge in dentistry. Previous periosteal distraction studies documented partial fill of the distraction space with newly-generated bone and fibrous connective tissue. Material and methods: Titanium meshes were inserted in subperiosteal tunnels in the calvaria of 20 rats through coronal incision. The devices were immediately activated after insertion by elevation of one side at 1 mm/day for 3 days. Rats were then divided into two groups (n = 10). Animals were sacrificed after 2 weeks (Group 1) and after 4 weeks (Group 2). Distraction sites specimens were embedded in paraffin and analyzed histologically and histomorphometrically. Results: In both groups, new periosteum was regenerated and covered the original bone surface in the distraction site. Distraction spaces showed a predomination of hyper-vascularized connective tissue and little new bone formation near to the stable end of the device. The 4-week findings showed more organized collagen fibers with less vascularity compared to the 2-week findings. Conclusion: The periosteal distraction technique can effectively regenerate connective tissue. It may open a new modality in the guided tissue regeneration for soft tissue augmentation.http://www.sciencedirect.com/science/article/pii/S1013905220307628Soft tissueAugmentationRegenerationPeriosteal distraction
spellingShingle Osama Zakaria
In situ soft tissue regeneration using periosteal distraction: A preliminary study in the rat calvarial model
Saudi Dental Journal
Soft tissue
Augmentation
Regeneration
Periosteal distraction
title In situ soft tissue regeneration using periosteal distraction: A preliminary study in the rat calvarial model
title_full In situ soft tissue regeneration using periosteal distraction: A preliminary study in the rat calvarial model
title_fullStr In situ soft tissue regeneration using periosteal distraction: A preliminary study in the rat calvarial model
title_full_unstemmed In situ soft tissue regeneration using periosteal distraction: A preliminary study in the rat calvarial model
title_short In situ soft tissue regeneration using periosteal distraction: A preliminary study in the rat calvarial model
title_sort in situ soft tissue regeneration using periosteal distraction a preliminary study in the rat calvarial model
topic Soft tissue
Augmentation
Regeneration
Periosteal distraction
url http://www.sciencedirect.com/science/article/pii/S1013905220307628
work_keys_str_mv AT osamazakaria insitusofttissueregenerationusingperiostealdistractionapreliminarystudyintheratcalvarialmodel